Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-29T19:31:58.434Z Has data issue: false hasContentIssue false
  • English
  • Français

Observations on the pattern on biohydrogenation of esterified and unesterified linoleic acid in the rumen

Published online by Cambridge University Press:  07 January 2011

R. C. Noble ,
J. H. Moore  and
C. G. Harfoot
R. C. Noble
Affiliation:
Hannah Research Institute, Ayr KA6 5HL
J. H. Moore
Affiliation:
Hannah Research Institute, Ayr KA6 5HL
C. G. Harfoot
Affiliation:
Hannah Research Institute, Ayr KA6 5HL
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Studies have been made of the effects of different concentrations of either free or esterified linoleic acid on the biohydrogenation of linoleic acid by rumen micro-organisms in vitro. A comparison has been made with the changes which occurred in the fatty acid compositions of rumen free fatty acids and plasma triglycerides of sheep given intraruminal infusions of linoleic acid or maize oil.

2. In the in vitro experiments, with increasing concentrations of 18:2 added as the free fatty acid, a decreasing proportion of this 18:2 was hydrogenated to 18:0 and trans-11-octadecenoic acid accumulated. The accumulation of large amounts of trans-11-octadecenoic acid was accompanied in all instances by the accumulation of a conjugated diene identified as cis-9, trans-11-octadecadienoic acid. There appeared to be a product–precursor relationship between the conjugated diene and the trans-11 monoene.

3. When linoleic acid was presented in vitro as the triglyceride, the extent to which hydrogenation occurred was, in all instances, greater than when equivalent amounts of 18:2 were presented as the free acid. Only small amounts of the cis-9, trans-11 diene were detected, and there was no apparent product–precursor relationship between this conjugated diene and the C18 monoenoic acids. The C18 monoenoic acids that accumulated consisted of both cis and trans isomers; the cis isomers consisted largely of cis-9- and cis-11-octadecenoic acids, which together comprised about 30% of the C18 monoenoic acids present.

4. The infusion of free linoleic acid into the rumen of sheep resulted in an increase in the proportion of total 18:1 and a decrease in the proportions of 16:0 and 18:0 in the total rumen free fatty acids. This increase which occurred in the concentration of 18:1 consisted predominantly of the trans-11 isomer. A concomitant increase in the concentration of the C18trans-11 acid was observed to occur in the fatty acids of the plasma triglycerides. Infusion of maize oil into the rumen of sheep resulted in little change in the fatty acid compositions of either the free fatty acids in the rumen or the triglycerides of the plasma.

5. The findings in vitro and in vivo are discussed with reference to each other and with reference to the possibility that biohydrogenation of 18:2 derived from the triglyceride proceeds by a different pathway from that of 18:2 presented as the free acid.

Type
Research Article
Information
British Journal of Nutrition , Volume 31 , Issue 1 , January 1974 , pp. 99 - 108
Copyright
Copyright © The Nutrition Society 1974

References

REFERENCES

Annison, E. F., Linzell, J. L., Fazakerley, S. & Nichols, B. W. (1967). Biochem. J. 102, 637.CrossRefGoogle Scholar
Chang, T.-C. L. & Sweeley, C. C. (1962). J. Lipid Res. 3, 170.CrossRefGoogle Scholar
Christie, W. W., Noble, R. C. & Moore, J. H. (1970). Analyst, Lond. 95, 940.CrossRefGoogle Scholar
Czerkawski, J. W. (1966). Br. J. Nutr. 20, 833.CrossRefGoogle Scholar
Czerkawski, J. W. (1967). Br. J. Nutr. 21, 865.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1969). Br. J. Nutr. 23, 51.CrossRefGoogle Scholar
Dawson, R. M. C. & Kemp, P. (1969). In Physiology of Digestion and Metabolism in the Ruminanant p. 504 [Phillipson, A. T. editor]. Newcastle-upon-Tyne: Oriel Press.Google Scholar
Demeyer, D. I. & Henderickx, H. K. (1967). Biochim. biophys. Acta 137, 484.CrossRefGoogle Scholar
Garton, G. A. (1965). In Physiology of Digestion in The Ruminant p. 390 [Dougherty, R. W. editor]. London: Butterworths.Google Scholar
Hawke, J. C. & Silcock, W. R. (1970). Biochim. biophys. Acta 218, 201.CrossRefGoogle Scholar
Lough, A. K. (1969). In Physiology of Digestion and Metabolism in the Ruminant p. 519 [Phillipson, A. T., editor[. Newcastle-upon-Tyne: Oriel Press.Google Scholar
McDougall, E. I. (1948). Biochem. J. 43, 99.CrossRefGoogle Scholar
Macleod, G. K., Wood, A. S. & Yao, Y. T. (1972). J. Dairy Sci. 55, 446.CrossRefGoogle Scholar
Moore, J. H., Noble, R. C. & Steele, W. (1968). Br. J. Nutr. 22, 681.CrossRefGoogle Scholar
Moore, J. H., Noble, R. C., Steele, W. & Czerkawski, J. W. (1969). Br. J. Nutr. 23, 869.CrossRefGoogle Scholar
Moore, J. H. & Williams, D. L. (1963). Can. J. Biochem. Physiol. 41, 1821.CrossRefGoogle Scholar
Moore, J. H. & Williams, D. L. (1966). Biochim. biophys. Acta 125, 352.CrossRefGoogle Scholar
Morris, L. J. (1966). J. Lipid Res. 7, 717.CrossRefGoogle Scholar
Nelson, G. J. & Freeman, N. K. (1959). J. biol. Chem. 234, 1375.CrossRefGoogle Scholar
Noble, R. C., Steele, W. & Moore, J. H. (1969). J. Dairy Res. 23, 709.Google Scholar
Polan, C. E., McNeill, J. J. & Tove, S. B. (1964). J. Bact. 88, 1056.CrossRefGoogle Scholar
Steele, W., Noble, R. C. & Moore, J. H. (1971). J. Dairy Res. 38, 49.CrossRefGoogle Scholar
Viviani, R. (1970). Adv. Lipid Res. 8, 267.CrossRefGoogle Scholar
Ward, P. F. V., Scott, T. W. & Dawson, R. M. C. (1964). Biochem. J. 92, 60.CrossRefGoogle Scholar
Wilde, P. F. & Dawson, R. M. C. (1966). Biochem. J. 98, 469.CrossRefGoogle Scholar