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Nutritional and endocrinological manipulation of lean deposition in forage-fed steers

Published online by Cambridge University Press:  09 March 2007

J. M. Dawson
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics LE12 5RD
P. J. Buttery
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics LE12 5RD
M. J. Lammiman
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics LE12 5RD
J. B. Soar
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics LE12 5RD
C. P. Essex
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics LE12 5RD
M. Gill
Affiliation:
AFRC Institute for Grassland and Animal Production, Hurley Research Station, Hurley, Maidenhead, Berks SL6 5LR
D. E. Beever
Affiliation:
AFRC Institute for Grassland and Animal Production, Hurley Research Station, Hurley, Maidenhead, Berks SL6 5LR
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Abstract

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The effect of supplementing grass silage with fishmeal on growth, muscle composition and the rate of muscle protein synthesis was investigated in young Friesian steers with and without oestradiol implants. The effect of the β-adrenergic agonist cimaterol was simultaneously investigated in animals fed on silage alone. Treatments lasted for 9 or 10 weeks. Fishmeal supplementation significantly increased animal growth rates (P < 0.001) and the weights of three dissected muscles (P < 0.001) compared with the silage-fed controls. These effects were further enhanced in animals also implanted with oestradiol. Muscle weights expressed as a proportion of body-weight were increased by fishmeal, suggesting that protein deposition had been enhanced. No further increase in the proportional muscle weights was obtained with oestradiol. Muscle dry matter content tended to be increased in both implanted and non-implanted animals receiving fishmeal compared with controls, but the proportions of protein, fat and ash were relatively constant. The intramuscular lipid composition was slightly altered by fishmeal. Muscle protein fractional synthetic rates (FSR), measured by continuous infusion of [3H]tyrosine, were increased by fishmeal in all three muscles of both implanted and non-implanted animals. There were no differences, however, due to oestradiol, over non-implanted fishmeal animals. This suggests that oestradiol may increase muscle accretion by reducing protein degradation rate. Cimaterol significantly increased longissimus dorsi (P < 0.05) and vastus lateralis (P < 0.01) muscle weights but had no effect on semitendinosus muscle weight or live-weight gain. The proportion of protein was increased (P <0.001) and the fat content reduced (P < 0.05) in all three muscles but intramuscular lipid composition was not markedly affected. Whilst methylhistidine: creatinine excretion was reduced by cimaterol, FSR were increased in the I. dorsi and v. lateralis muscles suggesting β-agonists have effects on both protein synthesis and protein degradation.

Type
Growth and Development
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Atkin, G. E. & Ferdinand, W. (1970). Accelerated amino acid analysis: studies on the use of lithium citrate buffers and the effect of n-propanol, in the analysis of physiological fluids and protein hydrolyzates. Analytical Biochemistry 38, 313329.CrossRefGoogle ScholarPubMed
Beermann, D. H., Fishell, V. K., Hogue, D. E., Ricks, C. A. & Dalrymple, R. H. (1985). Effects of the repartitioning agent, cimaterol (CL263, 780) on skeletal muscle fibre type and fibre hypertrophy in lambs. Journal of Animal Science 61, Suppl. 1, 254 Abstr.Google Scholar
Beever, D. E., Gill, M., Dawson, J. M. & Buttery, P. J. (1990). Effect of fishmeal on the digestion of grass silage by growing cattle. British Journal of Nutrition 63, 489502.CrossRefGoogle ScholarPubMed
Bohorov, O., Buttery, P. J., Correia, J. H. R. D. & Soar, J. B. (1987). The effect of the β-2-adrenergic agonist clenbuterol or implantation with oestradiol plus trenbolone acetate on protein metabolism in wether lambs. British Journal of Nutrition 57, 99107.CrossRefGoogle ScholarPubMed
Breier, B. H., Gluckman, P. D. & Bass, J. J. (1988 a). The somatotrophic axis in young steers: influence of nutritional status and oestradiol-17β on hepatic high- and low-affinity somatotrophic binding sites. Journal of Endocrinology 116, 169177.CrossRefGoogle ScholarPubMed
Breier, B. H., Gluckman, P. D. & Bass, J. J. (1988 b). Influence of nutritional status and oestradiol-17β on plasma growth hormone, insulin-like growth factors-I and -II and the response to exogenous growth hormone in young steers. Journal of Endocrinology 118, 243250.CrossRefGoogle ScholarPubMed
Bryant, D. T. W. & Smith, R. W. (1982). Protein synthesis in muscle of mature sheep. Journal of Agricultural Science 98, 639643.CrossRefGoogle Scholar
Christie, W. W. (1973). Lipid Analysis, pp. 108112. Oxford: Pergamon Press.Google Scholar
Claeys, M. C., Mulvaney, D. R., McCarthy, F. D., Gore, M. T., Marple, D. N. & Sartin, J. L. (1989). Skeletal muscle protein synthesis and growth hormone secretion in young lambs treated with clenbuterol. Journal of Animal Science 67, 22452254.CrossRefGoogle ScholarPubMed
Dawson, J. M., Bruce, C. I., Buttery, P. J., Gill, M. & Beever, D. E. (1988). Protein metabolism in the rumen of silage-fed steers: effect of fishmeal supplementation. British Journal of Nutrition 60, 339353.CrossRefGoogle ScholarPubMed
Dawson, J. M., Buttery, P. J., Beever, D. E., Gill, M., Lammiman, M. J., Soar, J. B. & Essex, C. P. (1987). Rates of muscle protein synthesis in silage-fed steers with manipulated carcass composition. In Proceedings of the 5th International Symposium on Protein Metabolism and Nutrition, European Association for Animal Production, Publication no. 35, pp. 5253 [Brauer, W., editor]. Rostock: Wissenschaftliche Zeitschrift der Wilhelm-Pieck Universität.Google Scholar
Eisemann, J. H., Hammond, A. C. & Rumsey, T. S. (1989). Tissue protein synthesis and nucleic acid concentrations in steers treated with somatotropin. British Journal of Nutrition 62, 657671.CrossRefGoogle ScholarPubMed
Emery, P. W., Rothwell, N. J., Stock, M. J. & Winter, P. D. (1984). Chronic effects of β2-adrenergic agonists on body composition and protein synthesis in the rat. Bioscience Reports 4, 8391.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497509.CrossRefGoogle ScholarPubMed
Forbes, G. B. & Bruining, G. J. (1976). Urinary creatinine excretion and lean body mass. American Journal of Clinical Nutrition 29, 13591366.CrossRefGoogle ScholarPubMed
Forsberg, N. E., Nassar, A. R. & Dalrymple, R. H. (1987). Cimaterol reduces Cathepsin B activity in sheep skeletal muscle. Federation Proceedings 46, 1176 Abstr.Google Scholar
Garlick, P. J. & Marshall, I. (1972). A technique for measuring brain protein synthesis. Journal of Neurochemistry 19, 577583.CrossRefGoogle ScholarPubMed
Garlick, P. J., Millward, D. J. & James, W. P. T. (1973). The diurnal response of muscle and liver protein synthesis in vivo in meal-fed rats. Biochemical Journal 136, 935945.CrossRefGoogle ScholarPubMed
Garlick, P. J., Millward, D. J., James, W. P. T. & Waterlow, J. C. (1975). The effect of protein deprivation and starvation on the rate of protein synthesis in tissues of the rat. Biochimica et Biophysica Acta 414, 7184.CrossRefGoogle ScholarPubMed
Gill, M., Beever, D. E., Buttery, P. J., England, P., Gibb, M. J. & Baker, R. D. (1987). The effect of oestradiol- 17β implantation on the response in voluntary intake, live-weight gain and body composition, to fishmeal supplementation of silage offered to growing calves. Journal of Agricultural Science, Cambridge 108, 916.CrossRefGoogle Scholar
Gopinath, R. & Kitts, W. D. (1984). Growth hormone secretion and clearance rate in growing beef steers implanted with oestrogenic anabolic compounds. Growth 48, 499514.Google Scholar
Harris, C. I. & Milne, G. (1981). The urinary excretion of N-methyl histidine by cattle: validation as an index of muscle protein breakdown. British Journal of Nutrition 45, 411422.CrossRefGoogle Scholar
Hunter, R. A., Davey, J. B. & Buttery, P. J. (1987). Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique. Journal of Agricultural Science, Cambridge 108, 511514.CrossRefGoogle Scholar
Jackson, M. J., Roberts, J. & Edwards, R. H. T. (1988). Dietary fish oil supplementation modifies rat skeletal muscle fatty acid composition but does not influence the response of muscles to experimental damage. Proceedings of the Nutrition Society 47, 32A.Google Scholar
Kim, Y. S., Lee, Y. B. & Ashmore, C. R. (1988). Cimaterol-induced growth in rats: growth pattern and biochemical characteristics. Growth, Development and Aging 52, 4146.Google ScholarPubMed
Kim, Y. S., Lee, Y. B. & Dalrymple, R. H. (1987). Effect of the repartitioning agent cimaterol on growth, carcass and skeletal muscle characteristics in lambs. Journal of Animal Science 65, 13921399.CrossRefGoogle ScholarPubMed
Lobley, G. E., Milne, V., Lovie, J. M., Reeds, P. J. & Pennie, K. (1980). Whole body and tissue protein synthesis in cattle. British Journal of Nutrition 43, 491502.CrossRefGoogle ScholarPubMed
Lonsdale, C. (1976). The effect of season of harvest on the utilisation by young cattle of dried grass given alone or as a supplement to silage. PhD thesis, University of Reading.Google Scholar
Miller, M. F., Garcia, D. K., Coleman, M. E., Ekeren, P. A., Lunt, D. K., Wagner, K. A., Procknor, M., Welsh, T. H. Jr & Smith, S. B. (1988). Adipose tissue, longissimus muscle and anterior pituitary growth and function in clenbuterol-fed heifers. Journal of Animal Science 66, 1220.CrossRefGoogle ScholarPubMed
Millward, D. J. & Bates, P. C. (1983). 3-Methylhistidine turnover in the whole body, and the contribution of skeletal muscle and intestine to urinary 3-methylhistidine excretion in the adult rat. Biochemical Journal 214, 607615.CrossRefGoogle ScholarPubMed
Moser, R. L., Dalrymple, R. H., Cornelius, S. G., Pettigrew, J. E. & Allen, C. E. (1986). Effect of cimaterol (CL 263,780) as a repartitioning agent in the diet for finishing pigs. Journal of Animal Science 62, 2126.CrossRefGoogle Scholar
Nicholas, G. A., Lobley, G. E. & Harris, C. I. (1977). Use of the constant infusion technique for measuring rates of protein synthesis in the New Zealand White rabbit. British Journal of Nutrition 38, 117.CrossRefGoogle ScholarPubMed
Osborne, D. R. & Voogt, P. (1978). The Analysis of Nutrients in Foods, pp. 156158. London: Academic Press.Google Scholar
Owen, J. A., Iggo, B., Scandrett, F. J. & Stewart, C. P. (1954). The determination of creatinine in plasma or serum, and in urine: a critical examination. Biochemical Journal 58, 426437.CrossRefGoogle ScholarPubMed
Pell, J. M. & Bates, P. C. (1987). Collagen and non-collagen protein turnover in skeletal muscle of growth hormone-treated lambs. Journal of Endocrinology 115, R1R4.CrossRefGoogle ScholarPubMed
Pell, J. M., Gill, M. & Beever, D. E. (1989). Variability of responsiveness to growth hormone in ruminants: nutrient interactions. In Use of Somatotropin in Livestock Production, [Sejrsen, K.Vestergaard, M. and Neimann-Sorensen, A., editors]. London: Elsevier Applied Science.Google Scholar
Quirke, J. F., Allen, P., Moloney, A. P., Sommer, M., Hanrahan, J. P., Sheehan, W. & Roche, J. F. (1988). Effects of the beta-agonist cimaterol on blood metabolite and hormone concentrations, growth and carcass composition in finishing Friesian steers. Journal of Animal Physiology and Animal Nutrition 60, 128136.CrossRefGoogle Scholar
Reeds, P. J., Hay, S. M., Dorwood, P. M. & Palmer, R. M. (1986). Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis. British Journal of Nutrition 56, 249258.CrossRefGoogle ScholarPubMed
Ricks, C. A., Dalrymple, R. H., Baker, P. K. & Ingle, D. L. (1984). Use of a β-agonist to alter fat and muscle deposition in steers. Journal of Animal Science 59, 12471255.CrossRefGoogle Scholar
Sinnett-Smith, P. A., Dumelow, N. W. & Buttery, P. J. (1983). Effects of trenbolone acetate and zeranol on protein metabolism in male castrate and female lambs. British Journal of Nutrition 50, 225234.CrossRefGoogle ScholarPubMed
Vernon, B. G. & Buttery, P. J. (1976). Protein turnover in rats treated with trenbolone acetate. British Journal of Nutrition 36, 575579.CrossRefGoogle Scholar
Waalkes, T. P. & Udenfriend, S. (1957). A fluorometric method for the estimation of tyrosine in plasma and tissues. Journal of Laboratory and Clinical Medicine 50, 733736.Google ScholarPubMed
Williams, P. E. V., Pagliani, L., Innes, G. M., Pennie, K., Harris, C. I. & Gaithwaite, P. (1987). Effects of a β-agonist (clenbuterol) on growth, carcass composition, protein and energy metabolism of veal calves. British Journal of Nutrition 57, 417428.CrossRefGoogle ScholarPubMed