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Non-essential nitrogen and protein utilization in the growing rat

Published online by Cambridge University Press:  09 March 2007

J. Heger
J. Heger
Affiliation:
Research Institute of Feed Supplements and Veterinary Drugs, Na Chvalce 2049, 193 00 Praha 9, Czechoslovakia
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Abstract

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Three series of nitrogen-balance experiments were carried out on growing rats fed on purified isonitrogenous diets (16 g N/kg) to study the importance of non-essential N and the essential:total N (E:T) ratio for attaining maximum N balance (NB) and biological value (BV) of protein. Minimum dietary levels of asparagine, proline and glutamic acid required for maximum NB and BV were estimated to be 1.0, 2.0 and 5.0 g/kg respectively. In an essential amino acid-based diet, the levels of individual amino acids were successively reduced to 110% of the requirement. Reducing the level of arginine, lysine or methionine + cystine resulted in a significant increase in NB and BV while the response of rats given the isoleucine-reduced diet significantly decreased. Addition of asparagine, proline and glutamic acid in the estimated minimum amounts to an essential amino acid-based diet resulted in a significant increase in NB and BV. A further significant increase was found when the levels of arginine, lysine and methionine + cystine in the diet were reduced to 110 % of the requirement. The performance of rats fed on the latter diet was similar to that of rats given a diet with the optimum E:T ratio. It is concluded that the optimum protein utilization may be influenced by the presence of some non-essential amino acids and by the surplus of some essential amino acids rather than by the E:T ratio per se.

Keywords

Protein utilizationNon-essential nitrogenRat
Type
Energy and Protein Metabolism
Information
British Journal of Nutrition , Volume 64 , Issue 3 , November 1990 , pp. 653 - 661
Copyright
Copyright © The Nutrition Society 1990

References

Adkins, J. S., Wertz, J. M., Boffman, R. H. & Hove, E. L. (1967). Influence of addition of water or ‘nonessential’ nitrogen on growth of rats fed low levels of essential L-amino acids. Proceedings of the Society for Experimental Biology and Medicine 126, 500–504.Google Scholar
Adkins, J. S., Wertz, J. M. & Hove, E. L. (1966). Influence of nonessential L-amino acids on growth of rats fed high levels of essential amino acids. Proceedings of the Society for Experimental Biology and Medicine 122, 519–523.Google Scholar
Allen, N. K. & Baker, D. H. (1974). Quantitive evaluation of nonspecific nitrogen sources for the growing chick. Poultry Science 53, 258–264.Google Scholar
Aqvist, S. E. G. (1951). Metabolic interrelationships among amino acids studied with isotopic nitrogen. Acta Chemica Scandinavica 5, 1046–1064.Google Scholar
Association of Official Agricultural Chemists (1970). Official Methods of Analysis, pp. 800–801. Washington, DC: Association of Official Agricultural Chemists.Google Scholar
Ball, R. D., Atkinson, J. L. & Bayley, H. S. (1986). Proline as an essential amino acid for the young pig. British Journal of Nutrition 55, 659–668.CrossRefGoogle ScholarPubMed
Bedford, M. R. & Summers, J. D. (1985). Influence of the ratio of essential to non essential amino acids on performance and carcase composition of the broiler chick. British Poultry Science 26, 483–491.Google Scholar
Benton, D. A., Harper, A. E., Spivey, H. E. & Elvehjem, C. A. (1956). Leucine, isoleucine and valine relationships in the rat. Archives of Biochemistry and Biophysics 60, 147–155.CrossRefGoogle ScholarPubMed
Berg, C. P. & Kolenbrander, H. M. (1970). Nitrogen metabolism in the mammal. In Comparative Biochemistry of Nitrogen Metabolism, pp. 795–916 [Campbell, J. W., editor]. London: Academic Press.Google Scholar
Birnbaum, S. M., Winitz, M. & Greenstein, J. P. (1957). Quantitative nutritional studies with water-soluble, chemically defined diets. III. Individual amino acids as sources of ‘non-essential’ nitrogen. Archives of Biochemistry and Biophysics 72, 428–436.Google Scholar
Breuer, L. H., Pond, W. G., Warner, R. G. & Loosli, J. K. (1964). The role of dispensable amino acids in the nutrition of the rat. Journal of Nutrition 82, 499–506.Google Scholar
Breuer, L. H., Warner, R. G., Benton, D. A. & Loosli, J. K. (1966). Dietary requirement for asparagine and its metabolism in rats. Journal of Nutrition 88, 143–150.Google Scholar
Chesney, R. W., Gusowski, N., Dabbagh, S. & Padilla, M. (1985). Renal cortex taurine concentration regulates renal adaptive response to altered dietary intake of sulfur amino acids. In Taurine, Biological Actions and Clinical Perspectives, pp. 32–43 [Oja, S. S., Ahtee, L. and Konro, P., editors]. New York: Liss.Google Scholar
Crosby, L. O. & Cline, T. R. (1973). Effect of asparagine on growth and protein synthesis in weanling rats. Journal of Animal Science 37, 713–717.Google Scholar
Greene, D. E. (1960). Factors influencing the growth of chicks fed crystalline amino acid diets with special reference to a growth stimulating factor in intact proteins. Dissertation Abstracts 21, 1312.Google Scholar
Greenstein, J. P., Birnbaum, S. M., Winnitz, M. & Otey, M. C. (1957). Quantitative nutritional studies with water-soluble chemically defined diet. I. Growth, reproduction and lactation in rats. Archives of Biochemistry and Biophysics 72, 396–416.Google Scholar
Harper, A. E. (1964). Amino acid toxicities and imbalances. In Mammalian Protein Metabolism, vol. 2, pp. 87–134. [Munro, H. N. and Allison, J. B., editors]. New York and London: Academic Press.Google Scholar
Harper, A. E., Benton, D. A. & Elvehjem, C. A. (1955). L-Leucine, an isoleucine antagonist in the rat. Archives of Biochemistry and Biophysics 57, 1–12.Google Scholar
Heger, J., Frydrych, Z. & Fron$$$k, P. (1983). Evaluation of optimum lysine and threonine supplements to a wheat and barley-based diet in rats. Animal Feed Science and Technology 8, 163–176.Google Scholar
Heger, J., Frydrych, Z. & Fronˇk, P. (1987). The effect of nonessential nitrogen on the utilization of dietary protein in the growing rat. Journal of Animal Physiology and Animal Nutrition 57, 130–139.Google Scholar
Hepburn, F. N. & Bradley, W. B. (1964). The glutamic acid and arginine requirement for high growth rate of rats fed amino acid diets. Journal of Nutrition 84, 305–312.CrossRefGoogle ScholarPubMed
Hepburn, F. N., Calhoun, W. K. & Bradley, W. B. (1960). A growth response of rats to glutamic acid when fed an amino acid diet. Journal of Nutrition 72, 163–171.Google Scholar
Itoh, H., Kishi, T., Emma, M. & Chibata, I. (1976). Nitrogen source primarily supplied by amino acids and the efficacy for maximal growth of rats. Journal of Nutritional Science and Vitaminology 22, 457–466.CrossRefGoogle ScholarPubMed
Matsuno, N., Yamaguchi, M., Saiki, R. & Tamura, E. (1976). Body weight change and nitrogen efficiencies in growing and adult rats fed diets containing various proportions of essential amino acids to total amino acids. Journal of Nutritional Science and Vitaminology 22, 321–331.Google Scholar
National Research Council (1978). Nutrient Requirements of the Laboratory Rat, pp. 13–26. Washington, DC: National Academy of Sciences.Google Scholar
Newburg, D. S., Frankel, D. L. & Fillios, L. C. (1975). An asparagine requirement in young rats fed the dietary combinations of aspartic acid, glutamine, and glutamic acid. Journal of Nutrition 105, 356–363.Google Scholar
Pegg, A. E. & McCain, P. P. (1982). Polyamine metabolism and function. American Journal of Physiology 243, C212–C221.Google Scholar
Ranhotra, G. S. & Johnson, B. C. (1965). Effect of feeding different amino acid diets on growth rate and nitrogen retention of weanling rats. Proceedings of the Society for Experimental Biology and Medicine 11, 1197–1201.CrossRefGoogle Scholar
Rogers, Q. R., Chen, D. M.-Y. & Harper, A. E. (1970). The importance of dispensable amino acids for maximal growth in the rat. Proceedings of the Society for Experimental Biology and Medicine 134, 517–522.Google Scholar
Stein, T. P., Settle, R. G., Albina, J. A., Dempsey, D. T. & Melnick, G. (1986). Metabolism of nonessential 15N- labelled amino acids and the measurement of human whole-body protein synthesis rates. Journal of Nutrition 116, 1651–1659.Google Scholar
Stipanuk, M. H. (1986). Metabolism of sulfur-containing amino acids. Annual Review of Nutrition 6, 179–209.Google Scholar
Stucki, W. P. & Harper, A. E. (1961). Importance of dispensable amino acids for normal growth of chicks. Journal of Nutrition 74, 377–383.Google Scholar
Stucki, W. P. & Harper, A. E. (1962). Effects of altering the ratio of indispensable to dispensable amino acids in diets for rats. Journal of Nutrition 78, 278–286.Google Scholar
Sugahara, M. & Ariyoshi, S. (1967). The nonessentiality of glycine and the essentiality of L-proline in the chick nutrition. Agricultural and Biological Chemistry 31, 106–110.Google Scholar
Sugahara, M. & Ariyoshi, S. (1968). The role of dispensable amino acids for the maximum growth of chicks. Agricultural and Biological Chemistry 32, 153–160.Google Scholar
Womack, M. (1969). Amino acid feeding studies: effect of various nonessential nitrogen sources and of added water. Proceedings of the Society for Experimental Biology and Medicine 131, 977–979.Google Scholar