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No effect of glucagon-like peptide-1 on short-term satiety and energy intake in man

Published online by Cambridge University Press:  09 March 2007

S. J. Long ,
J. A. Sutton ,
W. B. Amaee ,
A. Giouvanoudi ,
N. M. Spyrou ,
P. J. Rogers  and
L. M. Morgan
S. J. Long
Affiliation:
School of Biological Sciences University of Surrey, Guildford, GU2 5XH, UK
J. A. Sutton
Affiliation:
Guildford Clinical Pharmacology Unit Royal Surrey County Hospital, Guildford, GU2 5XX, UK
W. B. Amaee
Affiliation:
Department of Physics University of Surrey, Guildford, GU2 5XH, UK
A. Giouvanoudi
Affiliation:
Department of Physics University of Surrey, Guildford, GU2 5XH, UK
N. M. Spyrou
Affiliation:
Department of Physics University of Surrey, Guildford, GU2 5XH, UK
P. J. Rogers
Affiliation:
Institute of Food Research Reading Laboratory, Reading RG6 2EF, UK
L. M. Morgan*
Affiliation:
School of Biological Sciences University of Surrey, Guildford, GU2 5XH, UK
*
*Corresponding author:Dr Linda M. Morgan, fax +44 (0)1483 576978, email [email protected]
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Abstract

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Centrally administered glucagon-like peptide-1 (GLP-1) inhibits feeding in fasted rats, but its role in human satiety has been largely unexplored. The present study investigated the effect of peripheral GLP-1 infusion on gastric emptying and satiety in man. Ten non-obese male subjects were infused in a randomized single-blind within-subject crossover study using saline infusion as control. They received either a GLP-1 infusion (1·2 pmol/kg per min) or a saline infusion for 1 h, at 18.00 hours. At 20 min after starting the infusion the gastric emptying of a 400 ml water load was measured. Subjects completed behavioural self-rating scales to assess hunger and satiety. After 40 min subjects were given a buffet meal ad libitum and their food intake was recorded. GLP-1 infusion raised circulating GLP-1 concentrations to approximately twice those seen following a meal. It did not affect circulating insulin levels but caused a small fall in glucose levels. Gastric emptying of the water load was significantly delayed by the GLP-1 infusion. Energy intake from the buffet was unaffected by GLP-1 infusion. Self-assessment of hunger and satiety was similarly unaffected by the infusion before the buffet meal, although subjects tended to be less hungry after the buffet meal following GLP-1 infusion (P < 0·09). GLP-1 infusion delayed gastric emptying but had a minimal effect on food intake and satiety. This study casts doubts on whether GLP-1 is a major satiety factor in man, although a raised circulating plasma glucose level, as would normally occur postprandially, might be necessary for GLP-1 to increase satiety.

Keywords

Glucagon-like peptide-1Food intakeSatietyGastric emptying
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 4 , April 1999 , pp. 273 - 279
Copyright
Copyright © The Nutrition Society 1999

References

American Oil Chemists' Society (1992) Determination of tocopherols and tocotrienols in vegetable oils and fats by HPLC. In Official Methods and Recommended Practices of the American Oil Chemists' Society, 5th ed., [Firestone, D, editor]. Champaign, IL: AOCS.Google Scholar
Artho, A, Grob, K & Mariani, C (1993) On-line LC-GC for the analysis of minor components in edible oils and fats — The direct method involving silylation. Fat Science Technology 95, 176–180.Google Scholar
Ayesh, R, Weststrate, JA, Drewitt, PA & Hepburn, PA (1999) Faecal short chain fatty acid and microflora content, faecal bacterial enzyme activity and plasma female sex hormones in healthy normolipidemic volunteers consuming a controlled diet either with or without a phytosterol-ester enriched margarine. Food and Chemical Toxicology (In the Press).CrossRefGoogle Scholar
Baker, VA, Hepburn, PA, Kennedy, SJ, Jones, PA, Lea, LJ, Sumpter, JP & Ashby, J (1999) Safety evaluation of phytosterol esters. Part 1. Assessment of oestrogenicity using a combination of in vivo and in vitro assays. Food and Chemical Toxicology 37, 13–22.CrossRefGoogle ScholarPubMed
Best, MM & Duncan, CH (1958) Effects of the esterification of supplemental cholesterol and sitosterol in the diet. Journal of Nutrition 65, 169–181.CrossRefGoogle ScholarPubMed
Cucherat, M & Boissel, JP (1993) Meta-analysis of results from clinical-trials on prevention of coronary heart-disease by lipid-lowering interventions. Clinical Trials and Meta-analysis 28, 109–129.Google Scholar
Farquhar, JW & Sokolow, M (1958) Response of serum lipids and lipoproteins of man to beta-sitosterol and safflower oil — A long term study. Circulation 17, 890–899.CrossRefGoogle Scholar
Friedewald, WT, Levy, RI & Frederickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clinical Chemistry 18, 499–502.CrossRefGoogle ScholarPubMed
Giovannucci, E, Ascherio, A, Rimm, EB, Stampfer, MJ, Colditz, GA & Willett, WC (1995) Intake of carotenoids and retinol in relation to risk of prostate cancer. Journal of the National Cancer Institute 87, 1767–1776.CrossRefGoogle ScholarPubMed
Gordon, MH & Miller, LAD (1997) Development of steryl ester analysis for the detection of admixtures of vegetable oils. Journal of the American Oil Chemists' Society 74, 505–510.CrossRefGoogle Scholar
Grob, K, Lanfranchi, M & Mariani, C (1989) Determination of free and esterified sterols and of wax esters in oils and fats by coupled liquid chromatography–gas chromotography. Journal of Chromatography 471, 397–405.CrossRefGoogle Scholar
Grootenhuis, PA, Westenbrink, S, Sie, CMTL, Neeling, JND, Kok, FJ & Bouter, LM (1995) A semiquantitative food frequency questionnaire for use in epidemiologic research among elderly: validation by comparison with dietary history. Journal of Clinical Epidemiology 48, 859–868.CrossRefGoogle ScholarPubMed
Gylling, HK, Puska, P, Vartiainen, E & Miettinen, TA (1996) Serum retinol, α-tocopherol, carotenes and lipid peroxide production during serum cholesterol lowering by sitostanol ester margarine in a mildly hypercholesterolemic population. Circulation 94, Suppl. I-578, Abstr 3379.Google Scholar
Hendriks, HFL, Weststrate, JA, van Vliet, T & Meijer, GW (1999) Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects. European Journal of Clinical Nutrition 53, 319–327.CrossRefGoogle ScholarPubMed
Hepburn, PA, Horner, SA & Smith, M (1999) Safety evaluation of phytosterol esters. Part 2. Subchronic 90-day oral toxicity study on phytosterol-esters — a novel functional food Food and Chemical Toxicology (In the Press).CrossRefGoogle Scholar
Jandacek, RJ, Webb, MR & Mattson, FH (1977) Effect of an aqueous phase on the solubility of cholesterol in an oil phase. Journal of Lipid Research 18, 203–210.CrossRefGoogle Scholar
Kane, JP & Malloy, MJ (1982) Treatment of hypercholesterolemia. Medical Clinics of North America 66, 537–550.CrossRefGoogle ScholarPubMed
Law, MR, Wald, MJ & Thompson, SG (1994) By how much and how quickly does a reduction in serum cholesterol concentrations lower risk of ischaemic heart disease?. British Medical Journal 308, 367–372.CrossRefGoogle ScholarPubMed
Lees, AM, Mok, HYI, Lees, RS, McCluskey, MA & Grundy, SM (1977) Phytosterols as cholesterol-lowering agents: clinical trials in patients with hypercholesterolemia and studies of sterol balance. Atherosclerosis 28, 325–338.CrossRefGoogle ScholarPubMed
Levine, GN, Keany, JF & Vita, JA (1995) Cholesterol reduction in cardiovascular disease. New England Journal of Medicine 332, 512–521.CrossRefGoogle ScholarPubMed
Ling, WH & Jones, PJH (1995) Minireview dietary phytosterols: a review of metabolism, benefits and side effects. Life Sciences 57, 195–206.CrossRefGoogle Scholar
Mayne, ST (1996) Beta-carotene, carotenoids, and disease prevention in humans. FASEB Journal 10, 690–701.CrossRefGoogle ScholarPubMed
Mensink, RP & Katan, MB (1992) Effect of dietary fatty-acids on serum-lipids and lipoproteins — a meta-analysis of 27 trials. Arteriosclerosis and Thrombosis 12, 911–919.CrossRefGoogle ScholarPubMed
Miller, RG (1981) Simultaneous Statistical Interference. New York, NY: Springer Verlag.CrossRefGoogle Scholar
Nebeling, LC, Forman, MR, Graubard, BI & Snyder, RA (1997) Changes in the carotenoid intake in the United States: The 1987 and 1992 National Health Interview Surveys. Journal of the American Dietetic Association 97, 991–996.CrossRefGoogle ScholarPubMed
Oster, P, Schlierf, G, Heuck, CC, Greten, H, Gundert-Remy, U, Haase, W, Klose, G, Nothelfer, A, Raetzer, H, Schellenberg, B & Schmidt-Gayk, H (1976) Sitosterol in familial hyperlipoproteinemia type-2. Deutsche Medizinische Wochenschrift 101, 1308–1311.CrossRefGoogle Scholar
Peterson, DW, Shneour, EA, Peek, NF & Gaffey, HW (1953) Dietary constituents affecting plasma and liver cholesterol in cholesterol-fed chicks. Journal of Nutrition 50, 191–201.CrossRefGoogle ScholarPubMed
Pelletier, X, Belbraouet, S, Mirabel, D, Mordret, F, Perrin, JL, Pages, X & Debry, G (1995) A diet moderately enriched in phytosterols lowers plasma cholesterol concentrations in normocholesterolemic humans. Annals of Nutrition and Metabolism 39, 291–295.CrossRefGoogle ScholarPubMed
Plank, C & Lorbeer, E (1993) Analysis of free and esterified sterol in vegetable oil methyl esters by capillary gas chromatography. Journal of High Resolution Chromatography 16, 483–487.CrossRefGoogle Scholar
Pollak, OJ & Kritchevsky, D (1981) Sitosterol. Monographs on Atherosclerosis. Basel: S. Karger.Google Scholar
Probstfield, JL & Rifkind, BM (1991) The lipid-research-clinics coronary primary prevention trial — design, results, and implications. European Journal of Clinical Pharmacology 40, S69–S75.CrossRefGoogle ScholarPubMed
Reitsma, JB (1995) Hart- en vaatziekten in Nederland 1995: cijfers over ziekte en sterfte (Cardiovascular Diseases in The Netherlands 1995: Morbidity and Mortality Numbers). The Hague: Nederlandse Hartstichting.Google Scholar
Ripsin, CM, Keenan, JM, Jacobs, DR, Elmer, PJ, Welch, RR, Van Horn, L, Kiang Liu Turnbull, WH, Thye, FW, Kestin, M, Hegsted, M, Davidson, DM, Davidson, MH, Dugan, LD, Demark-Wahnefried, W & Beling, S (1992) Oat products and lipid lowering. Journal of the American Medical Association 267, 3317–3325.CrossRefGoogle ScholarPubMed
Salen, G, Shefer, S, Lien, NY, Ness, GC, Tint, GS & Shore, V (1992) Sitosterolemia. Journal of Lipid Research 33, 945–955.CrossRefGoogle ScholarPubMed
Schwartzkopff, W & Jantke, HJ (1978) Dose-effect of beta-sitosterin in type-IIA and type-IIB hypercholesterolemias. Munchener Medizinische Wochenschrift 120, 1575–1578.Google Scholar
Smith, SJ, Cooper, GR, Myers, GL & Sampson, EJ (1993) Biological variability in concentrations of serum lipids: sources of variation among results from published studies and composite predicted values. Clinical Chemistry 39, 1012–1022.CrossRefGoogle ScholarPubMed
Stampfer, MJ, Sacks, FM, Salvani, S, Willett, WC & Hennekens, CH (1991) A prospective study of cholesterol, apolipoproteins, and the risk of myocardial infarction. New England Journal of Medicine 325, 373–381.CrossRefGoogle ScholarPubMed
Steinberg, D & Witztum, L (1990) Lipoproteins and atherogenesis. Current concepts Journal of the American Medical Association 264, 3047–3052.CrossRefGoogle ScholarPubMed
Swell, L, Boiter, TA, Field, H & Treadwell, CR (1956) The absorption of plant sterols and their effect on serum and liver sterol levels. Journal of Nutrition 58, 385–395.CrossRefGoogle ScholarPubMed
Tang, JL, Armitage, JM, Lancaster, T, Silagy, CA, Fowler, GH & Neil, HAW (1998) Systematic review of dietary intervention trials to lower blood total cholesterol in free-living subjects. British Medical Journal 316, 1213–1220.CrossRefGoogle ScholarPubMed
Weststrate, JA, Ayesh, R, Bauer-Plank, C & Drewitt, PN (1999) Safety evaluation of phytosterol-esters part 4: faecal concentrations of bile acids and neutral sterols in healthy normolipidemic volunteers consuming a controlled diet either with or without a phytosterol-ester enriched margarine Food and Chemical Toxicology (In the Press).CrossRefGoogle Scholar
Weststrate, JA & Meijer, GW (1998) Plant sterol-enriched margarines and reduction of plasma total- and LDL-cholesterol concentrations in normocholesterolaemic and mildly hypercholesterolaemic subjects. European Journal of Clinical Nutrition 52, 334–343.CrossRefGoogle ScholarPubMed
Weststrate, JA & van het Hof, KH (1995) Sucrose polyester and plasma carotenoid concentrations in healthy subjects. American Journal of Clinical Nutrition 62, 591–597.CrossRefGoogle ScholarPubMed
Yeum, KJ, Booth, SL, Sadowski, JA, Liu, C, Tang, G, Krinsky, NI & Russell, RM (1996) Human plasma carotenoid response to the ingestion of controlled diets high in fruits and vegetables. American Journal of Clinical Nutrition 64, 594–602.CrossRefGoogle Scholar