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Nitrite and nitrate levels in ileostomy effluent: effect of dietary change

Published online by Cambridge University Press:  09 March 2007

B. C. Radcliffe ,
C. Hall  and
W. E. W. Roediger
B. C. Radcliffe
Affiliation:
Cell Physiology Laboratory,The University of Adelaide at The Queen Elizabeth Hospital, Adelaide, S.A. 5011, Australia
C. Hall
Affiliation:
Stomal Therapist The University of Adelaide at The Queen Elizabeth Hospital, Adelaide, S.A. 5011, Australia
W. E. W. Roediger
Affiliation:
Department of Surgery, The University of Adelaide at The Queen Elizabeth Hospital, Adelaide, S.A. 5011, Australia
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Abstract

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1. Nitrite and nitrate levels were measured in samples from ileostomy bags or stomal samples of thirty-one ileostomists (twenty-two ulcerative colitis, nine Crohn's disease), 14-16 h after ingestion of a conventional meal or a meal containing a high content of nitrite and nitrate.

2. Ileostomy samples were decolourized with barium chloride, sodium sulphate and charcoal. Nitrite was determined spectrophotometrically by the Griess reaction and nitrate determined as nitrite after reduction with nitrate reductase (ec 1.7.99.4) in the presence of sodium formate. The mean percentage recovery from twentysix spiked samples was 101.9 (se 3.5)% for nitrite and 82.9 (se 3.3)% for nitrate.

3. Ileostomy bag samples were obtained in twenty-nine cases of which ten had measurable nitrite (median 0, range 0-20.7 nmol/g) on a conventional meal compared with twenty-three cases (median 7.2, range 0-31.1 nmol/g) on the test meal (P < 0.01). Nitrate levels were measurable in sixteen (median 6.7, range 0-48.2 nmol/g) after a conventional meal compared with twenty-one (median 20.5, range 0-53.2 nmol/g) after the test meal (P <0.01).

4. Stomal fresh-catch samples were obtained in twenty-four cases: combined nitrate and nitrite was higher in eighteen, lower in four and unchanged in two subjects after the test meal (P < 0.05).

5. The type of foodstuff ingested can significantly alter measurable levels of nitrite-nitrate in the distal ileum and is one factor determining nitrite-nitrate input into the proximal colon.

Type
Research Article
Information
British Journal of Nutrition , Volume 61 , Issue 2 , March 1989 , pp. 323 - 330
Copyright
Copyright © The Nutrition Society 1989

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