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Morphological changes in hepatocytes of rats deprived of dietary nucleotides

Published online by Cambridge University Press:  09 March 2007

Ana T. Lopez-Navarro ,
Juan D. Bueno ,
Angel Gil  and
Antonio Sánchez-Pozo
Ana T. Lopez-Navarro
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
Juan D. Bueno
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
Angel Gil
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
Antonio Sánchez-Pozo
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
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Abstract

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The aim of the present study was to investigate the influence of dietary nucleotides on liver morphology. Adult rats were fed for 21 d on a nucleotide-containing diet or the same diet free of nucleotides. Liver sections were examined by light and transmission electron microscopy, as well as for nucleic acid and protein contents. Morphometric analysis was performed for different variables. Deprivation of dietary nucleotides resulted in a reduction in hepatocyte nuclear and nucleolar areas as well as in nuclear chromatin condensation. In addition, the rough endoplasmic reticulum was reduced, as were ribosome association and abundance, whereas fat accumulated. These findings portray dietary nucleotides as required nutrients for the liver under normal physiological conditions and suggest that an inadequate supply of nucleotides for a certain period of time has transient negative effects on liver ultrastructure and function.

Keywords

Dietary nucleotidesLiver
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 4 , October 1996 , pp. 579 - 589
Copyright
Copyright © The Nutrition Society 1996

References

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