Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-22T23:09:03.477Z Has data issue: false hasContentIssue false
  • English
  • Français

Monounsaturated n-9 fatty acids and adipocyte lipolysis in rats

Published online by Cambridge University Press:  09 March 2007

F. Soriguer ,
F. Moreno ,
G. Rojo-Martínez ,
E. García-Fuentes ,
F. Tinahones ,
J. M. Gómez-Zumaquero ,
A. L. Cuesta-Muñoz ,
F. Cardona  and
S. Morcillo
F. Soriguer*
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
F. Moreno
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
G. Rojo-Martínez
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
E. García-Fuentes
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
F. Tinahones
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
J. M. Gómez-Zumaquero
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
A. L. Cuesta-Muñoz
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
F. Cardona
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
S. Morcillo
Affiliation:
Endocrinology and Nutrition Service, ‘Carlos Haya’ Hospital Complex, Malaga-29009, Spain
*
*Corresponding Author: Dr Federico Soriguer, fax +34 952286704, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

To investigate the role of the monounsaturated n-9 fatty acids (MUFA) in the lipolytic activity of adipocytes, a study was carried out in which an increase in MUFA was produced in the tissues by two different methods; by the dietary enrichment of oleic acid or by producing an essential fatty acid deficiency syndrome. For this, forty-five male Sprague–Dawley rats were fed with a normal-energy diet and were subdivided into three groups. The diets varied in the type of dietary fat; palmitic acid, olive oil, or soyabean oil+palmitic acid. At the end of the study measurements were taken of weight, plasma leptin, tissue concentration of fatty acids, fat-cell size in the epididymal and the omental adipose tissues, adipocyte lipolytic activity of both tissues after stimulation with adrenaline, and the capacity of insulin to inhibit lipolysis. The baseline and adrenaline-stimulated lipolytic activity were greater and the anti-lipolytic capacity of insulin lower in the animals undergoing an increase in MUFA in the tissues (palmitic-acid and olive-oil diets). The area under the curve of glycerol, used as an indicator of lipolytic activity, was positively correlated with the concentration of MUFA and negatively with polyunsaturated fatty acids in the adipose tissues. It is concluded that an increase in tissue MUFA, however obtained, induces an increase in lipolytic activity.

Keywords

AdipocytesLipolysisMonounsaturated fatty acidsn-9 Fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 6 , December 2003 , pp. 1015 - 1022
Copyright
Copyright © The Nutrition Society 2003

References

Awad, AB & Chattopadhyay, JP (1986) Effect of dietary saturated fatty acids on hormone-sensitive lipolysis in rat adipocytes. J Nutr 116, 1088–1094.CrossRefGoogle ScholarPubMed
Axelrod, L, Shulam, GI, Bornstein, W, Roussel, AM & Aoki, TT (1986) Plasma level of, 13,14- dihydro -15-keto-PGE2 in patients with diabetic ketoacidosis and in normal fasting subjects. Diabetes 35, 104–110.10.2337/diab.35.9.1004CrossRefGoogle ScholarPubMed
Bergström, S & Carlson, LA (1965) Lipid mobilization in essential fatty-acid deficient rats: a preliminary note. Acta Physiol Scand 64, 479–480.CrossRefGoogle ScholarPubMed
Bizzi, A, Veneroni, E, Garattini, S, Puglisi, L & Paoletti, R (1967) Hypersensitivity to lipid mobilizing agents in essential fatty acid (EFA) deficient rat. Eur J Pharmacol 2, 48–52.10.1016/0014-2999(67)90022-2CrossRefGoogle Scholar
Bjerve, KS (1989) n-3 fatty acid deficiency in man. J Intern Med 225, Suppl. 1, 171–175.CrossRefGoogle Scholar
Bjerve, KS (1991) ω-3 fatty acid deficiency in man: implications for the requirement for α-linolenic acid and long-chain fatty acids. In Health Effects of Ω-3 Polyunsaturated Fatty Acids in Seafood, pp. 133–142 [Simopoulus, AP, Kiffer, RR, Martin, RE and Barlow, SM, editors]. World Review of Nutrition and Dietetics Basel, Switzerland: Karger Publishers.Google Scholar
Chatzipanteli, K, Rudolph, S & Axelrod, LL (1992) Coordinate control of lipolysis by prostaglandins E2 and prostacyclin in rat adipose tissue. Diabetes 41, 927–935.CrossRefGoogle ScholarPubMed
Christ, EJ & Nugteren, NH (1970) The biosynthesis and possible function of prostaglandins in adipose tissue. Biochim Biophys Acta 218, 296–307.CrossRefGoogle Scholar
Clandinin, MT, Foot, M & Robson, L (1983) Plasma membrane: can its structure and function be modulated by dietary fat? Comp Biochem Physiol 76B, 335–339.Google Scholar
Conner, WE, Lin, DS & Colvis, C (1996) Differential mobilization of fatty acids from adipose tissue. J Lipid Res 37, 290–298.10.1016/S0022-2275(20)37616-1CrossRefGoogle ScholarPubMed
DePury, GG & Collins, FD (1965) A raised level of free fatty acids in serum of rats deficient in essential fatty acids as a contributing cause of their fatty liver. Biochim Biophys Acta. 106, 213–214.10.1016/0005-2760(65)90113-XCrossRefGoogle Scholar
DiGirollamo, M, Mendlinger, S & Ferting, JW (1971) A simple method to determine fat cells size and number in four mammalians species. Am J Physiol 221, 850–855.CrossRefGoogle Scholar
Dulloo, AG & Giradier, L (1990) Adaptative changes in energy expenditure during refeeding following low-calorie intake: evidence for a specific metabolic component favoring fat storage. Am J Clin Nutr 52, 415–420.CrossRefGoogle ScholarPubMed
Eynard, AR, Jiang, WG & Mansel, RE (1998) Eicosatrienoic acid (20:3 n-9) inhibits the expression of E-cadherin and desmoglein in human squamous cell carcinoma in vitro. Prostaglandins Leukot Essent Fatty Acids 59, 371–377.CrossRefGoogle Scholar
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497–509.10.1016/S0021-9258(18)64849-5CrossRefGoogle ScholarPubMed
Fossati, P & Prencipe, L (1982) Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 28, 2077–2080.10.1093/clinchem/28.10.2077CrossRefGoogle ScholarPubMed
Goldrick, RB (1967) Morphological changes in the adipocyte during fat deposition and mobilization. Am J Physiol 212, 777–782.10.1152/ajplegacy.1967.212.4.777CrossRefGoogle ScholarPubMed
Hansen, RT, Wiese, HF & Boelsche, AN (1963) Role of linoleic acid in infant nutrition. Pediatrics 31, 171–192 Suppl., 171–192.CrossRefGoogle Scholar
Herrera, E, Amusquivar, E & Cacho, J. (2000) Changes in dietary fatty acids modify the decreased lipolytic beta-3 adrenergic response to hyperinsulinemia in adipocytes from pregnant and nonpregnant rats. Metabolism 49, 1180–1187.CrossRefGoogle ScholarPubMed
Hill, JO, Peters, JC, Lin, D, Yakubu, F, Geene, H & Swift, L (1993) Lipid accumulation and body fat distribution is influenced by type of dietary fat fed to rats. Int J Obes 17, 223–236.Google ScholarPubMed
Hjelte, L, Ahren, B, Andrén-Sandberg, A, Böttcher, G & Strandvik, B (1990) Pancreatic function in the essential fatty acid deficiency rat. Metabolism 39, 871–875.10.1016/0026-0495(90)90135-YCrossRefGoogle Scholar
Holman, RT (1977) Essential fatty acids in human nutrition. Adv Exp Med Biol 83, 515–534.CrossRefGoogle ScholarPubMed
Holman, RT, Adams, CE & Nelson, R, et al. (1995) Patients with anorexia nervosa demonstrate deficiencies of selected essential fatty acids, compensatory changes in nonessential fatty acids and decreased fluidity of plasma lipids. J Nutr 125, 901–907.Google ScholarPubMed
Holman, RT, Johnson, SB & Hatch, T (1982) A case of human linoleic acid deficiency involving neurological abnormalities. Am J Clin Nutr 35, 617–623.10.1093/ajcn/35.3.617CrossRefGoogle ScholarPubMed
Holman, RT, Johnson, SB, Mercuri, O, Itarte, HJ, Rodrigo, MA & De Thomas, ME (1981) Essential fatty acids deficiency in malnourished children. Am J Clin Nutr 34, 1534–1539.10.1093/ajcn/34.8.1534CrossRefGoogle ScholarPubMed
Juhr, NC & Franke, J (1992) A method for estimating the available energy of incompletely digested carbohydrates in rats. J Nutr 122, 1425–1433.10.1093/jn/122.7.1425CrossRefGoogle ScholarPubMed
McMurchie, EJ, Pattern, GS, Charrock, JS & McLennan, PL (1987) The interaction of dietary fatty acid and cholesterol on catecholamine-induced adenylate-cyclase activity in the rat heart. Biochim Biophys Acta 898, 137–153.10.1016/0005-2736(87)90032-0CrossRefGoogle Scholar
Makrides, M, Neumann, M, Simmer, K, Peters, J & Gibson, R (1995) Are long-chain polyunsaturated fatty acids essential nutrients in infancy? Lancet 345, 1463–1468.10.1016/S0140-6736(95)91035-2CrossRefGoogle ScholarPubMed
Martínez, JA, Aguado, M & Fruhbeck, G (2000) Interactions between leptin and NPY affecting lipid mobilization in adipose tissue. J Physiol Biochem 56, 1–8.CrossRefGoogle ScholarPubMed
Mersman, HJ, McNeel, RL, Morkeberg, JC, Shparber, A & Hachey, DL (1992) Beta adrenergic receptor-mediated functions in porcine adipose tissue are not affected differently by saturated vs unsaturated dietary fats. J Nutr 122, 1952–1959.CrossRefGoogle Scholar
Nestle, M (1995) Mediterranean diets: science and policy implications. Clin Nutr 61, 1313S.Google Scholar
Nicolas, C, Demarne, Y, Lecoutier, MJ & Lhuillery, C (1990) Specific alterations in different adipose tissues of pigs adipocyte plasma membrane structure by dietary lipids. Int J Obes 14, 537–549.Google ScholarPubMed
Obici, S, Feng, Z, Morgan, K, Stein, D, Karkanias, G & Rossetti, L (2002) Central administration of oleic acid inhibits glucose production and food intake. Diabetes 51, 271–275.CrossRefGoogle ScholarPubMed
Parrish, CC, Pathy, DA, Parkes, JG & Angel, A (1991) Dietary fish oils modify adipose structure and function. J Cell Physiol 148, 493–502.CrossRefGoogle ScholarPubMed
Portillo, MP, Tueros, AI, Perona, JS, Ruiz-Gutierrez, V, Torres, I & Macarulla, MT (1999) Modification induced by dietary lipid source in adipose tissue phospholipid fatty acids and their consequences in lipid mobilization. Br J Nutr 82, 319–327.CrossRefGoogle ScholarPubMed
Raclot, T & Oudart, H (1999) Selectivity of fatty acids on lipid metabolism and gene expression. Proc Nutr Soc 58, 633–646.CrossRefGoogle ScholarPubMed
Raclot, T & Oudart, H (2000) Net release of individual fatty acids from white adipose tissue during lipolysis in vitro: evidence for selective fatty acid re-uptake. Biochem J 348, 129–136.10.1042/bj3480129CrossRefGoogle ScholarPubMed
Robdell, M (1964) Metabolism of isolated fat cells. I: Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239, 375–380.Google Scholar
Rodríguez, VM, Picó, C, Portillo, MP, Maraculla, T & Palou, A, (2002 a) Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. Br J Nutr 87, 427–434.10.1079/BJN2002570CrossRefGoogle ScholarPubMed
Rodríguez, VM, Portillo, MP, Picó, C, Maraculla, T & Palou, A (2002 b) Olive oil feeding up-regulates uncoupling protein genes in rat brown adipose tissue and skeletal muscle. Am J Clin Nutr 75, 213–220.CrossRefGoogle ScholarPubMed
Serra, Majem L, Ribas, L, Tresserras, R, Ngo, J & Salleras, L (1995) How could changes in diet explain coronary heart disease mortality in Spain? The Spanish paradox. Clin Nutr 61, 1351S–1359S.Google Scholar
Smith, U, Hammersten, J & Björntorp, P (1979) Regional differences and effect of weight reduction on human fat cells metabolism. Eur J Clin Invest 9, 327–332.CrossRefGoogle Scholar
Soriguer, F, Tinahones, F & Monzón, A, et al. (2000) Varying incorporation of fatty acids in muscle, adipose, pancreatic exocrine tissue and thymocytes in adult rats fed with diet rich in different fatty acids. Eur J Epidemiol 16, 585–594.10.1023/A:1007684808188CrossRefGoogle Scholar
Strandvik, B, Brönnegard, M, Gilljam, H & Carlstedt-Duke, J (1988) Relation between defective regulation of arachidonic acid release and symptoms in cystic fibrosis. Scand J Gastroenterol 143, 1–4.CrossRefGoogle ScholarPubMed
Su, W & Jones, PHJ (1993) Dietary fatty acid composition influence energy accretion in rats. J Nutr 123, 2109–2114.Google ScholarPubMed
Tinahones, FJ, Pareja, A, Soriguer, FJ, Gómez-Zumaquero, JM & Esteva, I (1999) Pancreatic islet function in rats after a diet deficient in essential fatty acids. Diabetes Nutr Metab 11, 314–319.Google Scholar
Vaughan, M & Steimberg, D (1963) Effect of hormones on lipolysis and esterification of free fatty acids during incubation of adipose tissue in vitro. J Lipid Res 4, 193–199.CrossRefGoogle ScholarPubMed
Wang, MY, Lee, Y & Unger, RH (1999) Novel form of lipolysis induced by leptin. J Biol Chem 274, 17541–17544.CrossRefGoogle ScholarPubMed
Zhang, M, Hausman, DB & Hausman, GJ (1993) The cooperation of adipocytes and stromal cells in the secretion of prostaglandins by rat adipose tissue is not influenced by diet. J Nutr 123, 1203–1207.CrossRefGoogle Scholar
Zinder, D & Hapiro, B (1971) Effect of cell size on epinephrine and ACTH-induced fatty acid release from isolated fat cells. J Lipid Res 12, 91–95.CrossRefGoogle ScholarPubMed