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Modulation of age-related changes in immune functions of protein-deficient senescence-accelerated mice by dietary nucleoside-nucleotide mixture supplementation

Published online by Cambridge University Press:  09 March 2007

Clement K. Ameho ,
Andrew A. Adjei ,
Keiko Yamauchi ,
Eric K. Harrison ,
Anil D. Kulkarni ,
Seiji Sato  and
Shigeru Yamamoto
Clement K. Ameho
Affiliation:
Department of Nutrition, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
Andrew A. Adjei
Affiliation:
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
Keiko Yamauchi
Affiliation:
Department of Nutrition, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
Eric K. Harrison
Affiliation:
Department of Adult Health, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
Anil D. Kulkarni
Affiliation:
Surgical Research Institute, Department of Surgery, St Louis School of Medicine, Missouri, USA
Seiji Sato
Affiliation:
Ohtsuka Pharmaceutical Co., Naruto 772, Japan
Shigeru Yamamoto
Affiliation:
Department of Nutrition, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
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Abstract

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In the present study we examined the immune-enhancing effect of a nucleoside-nucleotide mixture on the non-specific T-cell immune functions of senescence-accelerated mice (SAM) fed on a low-protein diet. The immune functions studied were in vitro thymic and splenic cell lymphoproliferative responses to phytohaemagglutinin, lipopolysaccharide and concanavalin A and their production of interleukin-2 (IL-2) and interferon-γ (INF-γ) in response to mitogen stimulation. SAMP8 mice aged 3 and 6 months were used. In each age group, mice were fed on diets containing either 50g casein/kg, 50 g casein/kg supplemented with 5 g nucleoside-nucleotide mixture/kg or 200 g casein/kg for 3 weeks. The supplemented 3- and 6-month-old mice had higher (P<O·O5) thymic and splenic cell counts compared with the low-protein group. In both age groups of mice, concanavalin A induced higher (P<0·05) total thymic and splenic lymphoproliferative responses for the nucleoside-nucleotide mixture-supplemented group compared with the 50 g casein/kg dietary groups. Thymic and splenic production of IL-2 was higher for the 3-month-old mice in both the supplemented and the 200 g casein/kg dietary groups. INF-γ production in the supplemented 3-month-old group and the 6-month-old 200 g casein/kg dietary group was higher (P<0·05) compared with the other groups. Overall the supplemented 3-month-old mice exhibited both higher lymphoproliferative responses and production of cytokines compared with the supplemented 6-month-old mice. The results indicate that early nucleoside-nucleotide mixture supplementation may enhance the immune response in protein-deprived SAMP8 mice

Keywords

Nucleoside-nucleotide mixtureImmune functionSAMP8
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 5 , May 1997 , pp. 795 - 804
Copyright
Copyright © The Nutrition Society 1997

References

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