Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-23T07:17:20.589Z Has data issue: false hasContentIssue false
  • English
  • Français

Modifications induced by dietary lipid source in adipose tissue phospholipid fatty acids and their consequences in lipid mobilization

Published online by Cambridge University Press:  09 March 2007

María P. Portillo ,
Ana I. Tueros ,
Javier S. Perona ,
Valentina Ruiz-Gutiérrez ,
Isabel Torres  and
M. Teresa Macarulla
María P. Portillo*
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Ana I. Tueros
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Javier S. Perona
Affiliation:
Instituto de la Grasa (C.S.I.C.), Avda Padre García Tejero 4, 41012 Sevilla, Spain
Valentina Ruiz-Gutiérrez
Affiliation:
Instituto de la Grasa (C.S.I.C.), Avda Padre García Tejero 4, 41012 Sevilla, Spain
Isabel Torres
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
M. Teresa Macarulla
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
*
*Corresponding author: Dr María de Puy Portillo, fax +34 945 130756, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The aim of the present work was to assess the influence of dietary lipid source on fatty acid phospholipid profiles and on lipid mobilization. Forty male Wistar rats were divided into four groups and fed on high-fat diets which provided olive oil, sunflower oil, palm oil or beef tallow. All rats received the same amount of energy to avoid hyperphagia and differences in energy intake among groups. Phospholipid fatty acids were determined by GC. Lipolysis was stimulated in subcutaneous and perirenal isolated adipocytes by several lipolytic agents, and assessed by the determination of released glycerol. After 4 weeks of feeding, differences in body and adipose tissue weights were not observed. Dietary regimens caused great changes in adipose tissue phospholipid composition: rats fed on palm oil and beef tallow had higher concentrations of saturated fatty acids and animals fed on olive oil or sunflower oil had greater amounts of oleic and linoleic acids, respectively. These modifications did not lead to important changes in adipocyte lipolysis. Significant differences were only observed between palm-oil- and beef-tallow-fed groups when lipolysis was stimulated by isoproterenol in subcutaneous adipocytes. The fact that our feeding protocol did not induce differences in fat accumulation among groups avoids misinterpretations due to adiposity changes. The differences observed between both saturated-fat-fed groups, therefore, should only be attributable to dietary lipids. Despite this effect, the data from this work indicate that some diet-induced changes in adipose tissue fatty acid composition may have little effect on overall function.

Keywords

Fatty acidsAdipose tissueLipolysisPhospholipids
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 4 , October 1999 , pp. 319 - 327
Copyright
Copyright © The Nutrition Society 1999

References

Awad, AB, Bernardis, LL & Fink, CS (1990) Failure to demonstrate an effect of dietary fatty acid composition on body weight, body composition and parameters of lipid metabolism in mature rats. Journal of Nutrition 120, 1277–1282.Google Scholar
Awad, AB & Chattopadhyay, JP (1986 a) Effect of dietary saturated fatty acids on hormone-sensitive lipolysis in rat adipocytes. Journal of Nutrition 116, 1088–1094.CrossRefGoogle ScholarPubMed
Awad, AB & Chattopadhyay, JP (1986 b) Effect of dietary saturated fatty acids on intracellular free fatty acids and kinetic properties of hormone-sensitive lipase of rat adipocytes. Journal of Nutrition 116, 1095–1100.Google Scholar
Ayre, JK & Hulbert, AJ (1996) Dietary fatty acid profile influences the composition of skeletal muscle phospholipids in rats. Journal of Nutrition 126, 653–662.Google Scholar
Belzung, F, Raclot, T & Groscolas, R (1993) Fish oil n−3 fatty acids selectively limit hypertrophy of abdominal fat depots in growing rats fed high-fat diets. American Journal of Physiology 264, R1111–R1118.Google Scholar
Buller, KJ & Enser, M (1986) The effects of food intake and dietary fatty acids on the activity of staroyl-CoA Δ9-desaturase in pig adipose tissue. Journal of Agricultural Science, Cambridge 106, 601–609.Google Scholar
Camara, M, Mourot, J & Février, C (1996) Influence of two dietary fats on lipid synthesis in the pig: comparative study of liver, muscle and two back fat layers. Annals of Nutrition and Metabolism 40, 287–295.CrossRefGoogle Scholar
Carraro, R, Li, Z & Gregerman, RI (1994) Catecholamine-sensitive lipolysis in the rat: different loci for effect of age on the lipolytic cascade in epididymal vs perirenal fat cells. Journal of Gerontology 49, B140–B143.Google Scholar
Clandinin, MT, Foot, M & Robson, L (1983) Plasma membrane: can its structure and function be modulated by dietary fat?. Comparative Biochemistry and Physiology 76B, 335–339.Google Scholar
Clandinin, MT, Jumpsen, J & Suh, M (1994) Relationship between fatty acid accretion, membrane composition, and biological functions. Journal of Pediatrics 125, S25–S32.Google Scholar
Cunnane, SC (1996) Recent studies on the synthesis, beta-oxidation and deficiency of linoleate and alpha-linoleate: Are essential fatty acids aptly named indispensable or conditionally dispensable fatty acids?. Canadian Journal of Physiology and Pharmacology 74, 629–639.Google Scholar
Cunnane, SC & Anderson, MJ (1997) Pure linoleate deficiency in the rat: influence on growth, accumulation of n−6 polyunsaturates and [1-14C]linoleate oxidation. Journal of Lipid Research 38, 805–812.Google Scholar
Di Girolamo, M, Mendlinger, S & Fertig, JW (1971) A simple method to determine fat cell size and number in four mammalian species. American Journal of Physiology 221, 850–858.Google Scholar
Dole, VP & Meinertz, H (1960) Microdetermination of long chain fatty acids in plasma and tissues. Journal of Biological Chemistry 235, 2595–2599.CrossRefGoogle ScholarPubMed
Faust, IM, Johnson, PR, Stern, JS & Hirsch, J (1978) Diet-induced adipocyte number increase in adult rats: a new model of obesity. American Journal of Physiology 235, E279–E286.Google ScholarPubMed
Flatt, JP (1987) The difference in the storage capacities for carbohydrate and for fat, and its implications in the regulation of body weight. Annals of the New York Academy of Sciences 499, 104–123.Google Scholar
Folch, J, Lees, M & Sloane Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Hartman, AD, Cohen, AI, Richane, CJ & Hsu, T (1971) Lipolytic response and adenylate cyclase activity of rat adipocytes as related to cell size. Journal of Lipid Research 12, 498–505.CrossRefGoogle ScholarPubMed
Hill, JO, Lin, D, Yakubu, F & Peters, JC (1992) Development of dietary obesity in rats: influence of amount and composition of dietary fat. International Journal of Obesity 16, 321–333.Google Scholar
Hill, JO, Peters, JC, Lin, D, Yakubu, F, Greene, H & Swift, L (1993) Lipid accumulation and body fat distribution is influenced by type of dietary fat fed to rats. International Journal of Obesity 17, 223–236.Google ScholarPubMed
Houslay, MD (1985) Regulation of adenylate cyclase (EC 4.6.1.1) activity by its lipid environment. Proceedings of the Nutrition Society 44, 157–165.Google Scholar
Jensen, MD (1998) Diet effects on fatty acid metabolism in lean and obese humans. American Journal of Clinical Nutrition 67, Suppl. 3, 531S–534S.CrossRefGoogle ScholarPubMed
Khuu Thi-Dinh, KL, Demarne, Y, Nicolas, C & Lhuillery, C (1990) Effect of dietary fat on phospholipid class distribution and fatty acid composition in rat fat cell plasma membrane. Lipids 25, 278–283.CrossRefGoogle ScholarPubMed
Lafontan, M & Berlan, M (1993) Fat adrenergic receptor and the control of white and brown fat cell function. Journal of Lipid Research 34, 1057–1092.CrossRefGoogle ScholarPubMed
McMurchie, EJ (1988) Dietary lipids and the regulation of membrane fluidity and function. In Physiological Regulation of Membrane Fluidity, pp. 189–237 [Aloia, RC, editor]. New York, NY: Alan R. Liss.Google Scholar
McMurchie, EJ, Pattern, GS, Charrock, JS & McLennan, PL (1987) The interaction of dietary fatty acid and cholesterol on catecholamine-induced adenylate-cyclase activity in the rat heart. Biochimica et Biophysica Acta 898, 137–153.Google Scholar
Matsuo, T, Sumida, H & Suzuki, M (1995) Beef tallow diet decreases β-adrenergic receptor binding and lipolytic activities in different adipose tissues of rat. Metabolism 44, 1271–1277.CrossRefGoogle ScholarPubMed
Mersmann, HJ, McNeel, RL, Morkeberg, JC, Shparber, A & Hachey, DL (1992) β-adrenergic receptor-mediated functions in porcine adipose tissue are not affected differently by saturated vs unsaturated dietary fats. Journal of Nutrition 122, 1952–1959.CrossRefGoogle Scholar
Momchilova, A, Petkova, D, Mechev, I, Dimotrov, G & Koumanov, K (1985) Sensitivity of 5>-nucleotidase and phopholipase A2 towards liver plasma membrane modifications. International Journal of Biochemistry 17, 787–792.Google Scholar
Murphy, MG (1990) Dietary fatty acids and membrane protein function. Journal of Nutritional Biochemistry 1, 68–79.Google Scholar
National Research Council (1978) Nutrient Requirements of Laboratory Animals. Washington, DC: National Academy of Sciences.Google Scholar
Nicolas, C, Demarne, Y, Lecourtier, MJ & Lhuillery, C (1990) Specific alterations in different adipose tissues of pigs adipocyte plasma membrane structure by dietary lipids. International Journal of Obesity 14, 537–549.Google Scholar
Nicolas, C, Lacasa, D, Giudicelli, Y, Demarne, Y, Agli, B, Lecourtier, MJ & Lhuillery, C (1991) Dietary (n−6) polyunsaturated fatty acids affect β-adrenergic receptor binding and adenylate cyclase activity in pig adipocyte plasma membrane. Journal of Nutrition 121, 1179–1186.Google Scholar
Pan, DA & Storlien, LH (1993) Dietary lipid profile is a determinant of tissue phospholipid fatty acid composition and rate of weight gain in rats. Journal of Nutrition 123, 512–519.CrossRefGoogle ScholarPubMed
Parrish, CC, Pathy, DA, Parkes, JG & Angel, A (1991) Dietary fish oils modify adipose structure and function. Journal of Cell Physiology 148, 493–502.CrossRefGoogle ScholarPubMed
Portillo, MP, Serra, F, Simón, E, Del Barrio, AS & Palou, A (1998) Energy restriction with high-fat diet gives higher UCP1 and lower white fat in rats. International Journal of Obesity 22, 974–979.Google Scholar
Ruiz Gutiérrez, V, Molina, MT & Vázquez, CM (1990) Comparative effects of feeding different fats on fatty acid composition of major individual phospholipids of rat hearts. Annals of Nutrition and Metabolism 34, 350–358.Google Scholar
Shimomura, Y, Tamura, T & Suzuki, M (1990) Less body fat accumulation in rats fed a safflower oil diet than in rats fed a beef tallow diet. Journal of Nutrition 120, 1291–1296.Google Scholar
Spector, AA & York, MA (1985) Membrane lipid composition and cellular function. Journal of Lipid Research 26, 1015–1035.CrossRefGoogle ScholarPubMed
Su, W & Jones, PJH (1993) Dietary fat acid composition influences energy accretion in rats. Journal of Nutrition 123, 2109–2114.Google Scholar
Suárez, A, Ramírez, MC, Faus, MJ & Gil, A (1996) Dietary long-chain polyunsaturated fatty acids influence tissue fatty acid composition in rats at weaning. Journal of Nutrition 126, 887–897.Google Scholar
Sztalryd, C & Kraemer, FB (1994) Differences in hormone-sensitive lipase expression in white adipose tissue from various anatomic locations. Metabolism 43, 241–247.Google Scholar
Wieland, O (1957) Eine enzymatishe method zur bestimmung von glycerin (An enzymic method for the determination of glycerol). Biochemical Zeitung 239, 313–319.Google Scholar
Zinder, D & Saphiro, B (1971) Effect of cell size on epinephrine- and ACTH-induced fatty acid release from isolated fat cells. Journal of Lipid Research 12, 91–95.Google Scholar