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Milk-substitutes comparable to rat's milk; their preparation, composition and impact on development and metabolism in the artificially reared rat

Published online by Cambridge University Press:  09 March 2007

Nancy Auestad ,
Rose A. Korsak ,
James D. Bergstrom  and
John Edmond
Nancy Auestad
Affiliation:
Department of Biological Chemistry and the Mental Retardation Research Center, UCLA School of Medicine, Los Angeles, CA 90024-1737, USA
Rose A. Korsak
Affiliation:
Department of Biological Chemistry and the Mental Retardation Research Center, UCLA School of Medicine, Los Angeles, CA 90024-1737, USA
James D. Bergstrom
Affiliation:
Department of Biological Chemistry and the Mental Retardation Research Center, UCLA School of Medicine, Los Angeles, CA 90024-1737, USA
John Edmond
Affiliation:
Department of Biological Chemistry and the Mental Retardation Research Center, UCLA School of Medicine, Los Angeles, CA 90024-1737, USA
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Abstract

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1. Procedures are described to prepare nutritionally adequate rat milk-substitutes by modifying commercially available processed cow's milk, rich in carbohydrate and low in protein and fat compared with rat's milk.

2. Premilk formulas, prepared as intermediates in the preparation of rat milk-substitutes, are rich in protein but low in their concentration of fat, carbohydrate, and minerals when compared with rat's milk.

3. Premilks were supplemented with lactose, vitamins, minerals, fat as oil mixtures, certain amino acids and other constituents to yield rat milk-substitutes which resemble the known composition of rat's milk in their properties and composition.

4. Detailed analyses of the milk-substitutes show them to be comparable to rat's milk in energy content, pH, osmolarity, the concentration of the macronutrients, fat, protein and carbohydrate, and the major minerals.

5. Rat pups were artificially reared from postnatal day 4 or 5 until days 16–18 by fitting them with gastric cannulas through which the milk-substitutes could be infused automatically.

6. The nutritional impact of the milk-substitutes was assessed by a comparison of growth and mctabolic characteristics for artificially reared rats with age-matched sucking rats reared by their mother.

7. Indices which were taken to be appropriate included (a) body-weight gain; (b) the concentration in blood of protein, amino acids, ketone bodies, carnitine, glucose, galactose, lactate, insulin, and the electrolytes calcium, sodium, potassium and chloride; (c) the turnover of glucose and 3-hydroxybutyrate; (d) the concentration in brain of protein, cholesterol, cerebroside sulphate and the activities of the enzymes pyruvate dehydrogenase (EC 1.2.4.1), 3-oxo-acid-CoA transferase (EC 2.8.3.5) and acetoacetyl-CoA ligase (EC 6.2.1.16).

8. The studies suggest that milk-substitutes approximating to rat's milk in composition promote acceptable metabolism in the artificially reared rat pup.

Type
Protein, Growth and Development
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 495 - 518
Copyright
Copyright © The Nutrition Society 1989

References

Altman, P. L. (1961) Physical properties and chemical composition of milk. In Blood and Other Body Fluids pp. 453–466 [Dittmer, D. S, editor]. Washington, DC: Federation of American Societies for Experimental Biology.Google Scholar
Bailey, G. L., Clough, R. A. & Dodd, F. H. (1955) The rate of secretion of milk and fat. Journal of Dairy Research 22, 22–36.CrossRefGoogle Scholar
Bates, S. R. & Rothblat, G. H. (1974) Regulation of cellular sterol flux and synthesis by human serum lipoprotein. Biochimica et Biophysica Acta 360, 38–55.CrossRefGoogle Scholar
Bergstrom, J. D. & Edmond, J. (1985) A radiochemical assay for acetoacetyl-CoA synthetase. Analytical Biochemistry 149, 358–364.CrossRefGoogle ScholarPubMed
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Brandorff, N. P. (1980) The effect of dietary fat on the fatty acid composition of lipids secreted in rat's milk. Lipids 15, 276–277.CrossRefGoogle Scholar
Buckley, B. M. & Williamson, D. H. (1973) Acetoacetate and brain lipogenesis: developmental pattern of acetoacetyl-coenzyme A synthetase in the soluble fraction of rat brain. Biochemical Journal 132, 653–656.CrossRefGoogle ScholarPubMed
Chalk, P. A. & Bailey, E. (1979) Changes in the yield, and carbohydrate, lipid and protein content of milk during lactation in the rat. Journal of Developmental Physiology 1, 61–79.Google ScholarPubMed
Coward, W. A., Cole, T. J., Gerber, H., Roberts, S. B. & Fleet, I. (1982) Water turnover and the measurement of milk intake. European Journal of Physiology 393, 344–347.CrossRefGoogle ScholarPubMed
Cox, Jr., W., M. & Mueller, A. J. (1937) The composition of milk from stock rats and an apparatus for milking small laboratory animals. Journal of Nutrition 13, 249–261.CrossRefGoogle Scholar
Diaz, J., Moore, E., Petracca, F., Schacher, J. & Stamper, C. (1982) Artificial rearing of rat pups with a proteinenriched formula. Journal of Nutrition 112, 841–847.CrossRefGoogle ScholarPubMed
Diaz, J. & Schain, R. J. (1977) Chronic phenobarbital administration: effects upon brain growth and behaviour of artificially-reared rats. Proceedings of the Western Pharmacology Society 20, 153–157.Google ScholarPubMed
Diaz, J. & Schain, R. J. (1978) Phenobarbital: effects of long-term administration on behavior and brain of artificially-reared rats. Science 199, 90–91.CrossRefGoogle ScholarPubMed
Dobbing, J. (1968) Vulnerable periods in developing brain. In Applied Neurochemistry pp. 287–316 [Davison, A.N. and Dobbing, J., editors]. Oxford: Blackwell.Google Scholar
Dymsza, H. A., Czajka, D. M. & Miller, S. A. (1964) Influence of artificial diet on weight gain and body composition of the neonatal rat. Journal of Nutrition 84, 100–106.CrossRefGoogle ScholarPubMed
Eckhert, C. D. (1985) Isolation of a protein from human milk that enhances zinc absorption in humans. Biochemical and Biophysical Research Communications 130, 264–269.CrossRefGoogle ScholarPubMed
Edmond, J. (1974) Ketone bodies as precursors of sterols and fatty acids in the developing rat. Journal of Biological Chemistry 249, 72–80.CrossRefGoogle ScholarPubMed
Edmond, J., Auestad, N., Robbins, R. A. & Bergstrom, J. D. (1985) Ketone body metabolism in the neonate: development and the effect of diet. Federation Proceedings 44, 2359–2364.Google ScholarPubMed
Evans, G. W. (1980) Normal and abnormal zinc absorption in man and animals: the tryptophan connection. Nutrition Reviews 38, 137–141.CrossRefGoogle ScholarPubMed
Ezekiel, E. (1967) Intestinal iron absorption by neonates and some factors affecting it. Journal of Laboratory and Clinical Medicine 70, 138–149.Google Scholar
Forsum, E. & Lonnerdal, B. (1979) Variation in the contents of nutrients of breast milk during one feeding. Nutrition Reports International 19, 815–820.Google ScholarPubMed
Glass, R. L., Troolin, H. A. & Jenness, R. (1967) Comparative biochemical studies of milks. IV. Constituent fatty acids of milk fats. Comparative Biochemistry and Physiology 22, 415–425.CrossRefGoogle ScholarPubMed
Goda, T., Bustamante, S., Edmond, J., Grimes, J. & Koldovsky, O. (1985a) Precocious increase of sucrase activity by carbohydrates in the small intestine of suckling rats. II. Role of digestibility of sugars, osmolality, and stomach evacuation in producing diarrhea. Journal of Pediatric Gastroenterology and Nutrition 4, 634–638.Google ScholarPubMed
Goda, T., Yamada, K., Bustamante, S., Edmond, J., Grimes, J. & Koldovsky, O. (1985b) Precocious increase of sucrase activity by carbohydrates in the small intestine of suckling rats. I. Significance of the stress effect of sugar-induced diarrhea. Journal of Pediatric Gastroenterology and Nutrition 4, 468–475.Google Scholar
Godbole, V. Y., Grundleger, M. L., Pasquine, T. A. & Thenen, S. W. (1981) Composition of rat milk from day 5 to 20 of lactation and milk intake of lean and preobese Zucker pups. Journal of Nutrition 111, 480–487.CrossRefGoogle ScholarPubMed
Grigor, M. R. & Warren, S. M. (1980) Dietary regulation of mammary lipogenesis in lactating rats. Biochemical Journal 188, 61–65.CrossRefGoogle ScholarPubMed
Hall, W. G. (1975) Weaning and growth of artificially-reared rats. Science 190, 1313–1315.CrossRefGoogle ScholarPubMed
Hawkins, R. A., Williamson, D. H. & Krebs, H. A. (1971) Ketone-body utilization by adult and suckling rat brain in vivo. Biochemical Journal 122, 13–18.CrossRefGoogle ScholarPubMed
Hegsted, D. M. (1973) Calcium and phosphorus. In Modern Nutrition in Health and Disease 5th ed., pp. 268–286 [Goodhart, R.S. and Shils, M. E., editors]. Philadelphia: Lea and Febiger.Google Scholar
Henning, S. J. (1981) Postnatal development: coordination of feeding, digestion and metabolism. American Journal of Physiology 241, G199–G214.Google ScholarPubMed
Hytten, F. E. (1954) Clinical and chemical studies in human lactation. British Medical Journal i, 174–182.Google Scholar
Janas, L. M. (1985) Indices of protein metabolism in term infants fed human milk whey predominant formula, or cow's milk formula. Pediatrics 75, 775–784.CrossRefGoogle ScholarPubMed
Jenness, R. & Sloan, R. E. (1970) The composition of milks of various species: a review. Dairy Science Abstracts 32, 599–612.Google Scholar
Jones, M. R. (1988) Free amino acid pools in the spontaneously hypertensive rat. A longitudinal study. Journal of Nutrition 118, 579–587.CrossRefGoogle ScholarPubMed
Kean, E.L. (1968) Rapid, sensitive spectrophotometric method for quantitative determination of sulfatides. Journal of Lipid Research 9, 319–327.CrossRefGoogle ScholarPubMed
Keen, C. L., Lonnerdal, B., Clegg, M. & Hurley, L. S. (1981) Developmental changes in composition of rat milk: trace elements, minerals, protein, carbohydrate and fat. Journal of Nutrition 111, 226–230.CrossRefGoogle ScholarPubMed
Knight, C. H., Docherty, A. H. & Peaker, M. (1984) Milk yield in rats in relation to activity and size of the mammary secretory cell population. Journal of Dairy Research 51, 29–35.CrossRefGoogle ScholarPubMed
Kraus, H., Schlenker, S. & Schwedsky, D. (1974) Developmental changes of cerebral ketone body utilization in human infants. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 355, 164–170.Google ScholarPubMed
Kris-Etherton, P. M., Layman, D. K., York, P. V. & Frantz, I. D. (1979) The influence of early nutrition on the serum cholesterol of the adult rat. Journal of Nutrition 109, 1244–1257.CrossRefGoogle ScholarPubMed
Ksiezak-Reding, H., Blass, J. P. & Gibson, G. E. (1982) Studies on the pyruvate dehydrogenase complex in brain with the arylamine acetyl-transferase-coupled assay. Journal of Neurochemistry 38, 1627–1636.CrossRefGoogle ScholarPubMed
Kuhn, N. J. (1972) The lactose and neuramin lactose content of rat milk and mammary tissue. Biochemical Journal 130, 177–180.CrossRefGoogle Scholar
Land, J. M., Booth, R. F. G., Berger, R. & Clark, J. B. (1977) Development of mitochondrial energy metabolism in rat brain. Biochemical Journal 164, 339–348.CrossRefGoogle ScholarPubMed
Lonnerdal, B., Keen, C. L. & Hurley, L. S. (1978) Iron, copper, zinc and manganese in milk. Annual Review of Nutrition 1, 149–174.CrossRefGoogle Scholar
Lopes-Cardozo, M. & Klein, W. (1984) Ketone-body utilization and lipid synthesis by developing rat brain – a comparison between in vivo and in vitro experiments. Neurochemistry International 6, 459–466.CrossRefGoogle Scholar
Lopes-Cardozo, M., Koper, J. W., Klein, W. & Van Golde, L M. G. (1984) Acetoacetate is a cholesterogenic precursor for myelinating rat brain and spinal cord. Incorporation of label from [3-14C]acetoacetate, [14C]glucose and 3H2O. Biochimica et Biophysica Acta 794, 350–352.CrossRefGoogle Scholar
Luckey, T. D., Mende, T. J. & Pleasants, J. (1954) The physical and chemical characterization of rat's milk. Journal of Nutrition 54, 345–359.CrossRefGoogle ScholarPubMed
Messer, M., Thoman, E. B., Terrasa, A. G. & Dallman, P. R. (1969) Artificial feeding of infant rats by continuous gastric infusion. Journal of Nutrition 98, 404–410.CrossRefGoogle ScholarPubMed
Miller, S. A. & Czajka, D. M. (1967) The influence of dietary osmolarity on survival in the neonatal rat. Biology of the Neonate 11, 197–203.CrossRefGoogle ScholarPubMed
National Research Council (1978)Nutrient Requirements of Domestic Animals no. 10: Nutrient Requirements of Laboratory Animals 3rd ed. Washington DC: National Academy of Sciences.Google Scholar
Page, M. A., Krebs, H. A. & Williamson, D. H. (1971) Activities of enzymes of ketone body utilization in brain and other tissues of suckling rats. Biochemical Journal 121, 49–53.CrossRefGoogle ScholarPubMed
Patton, M. B. & Sutton, T. S. (1952) The utilization of calcium from lactate, gluconate, sulfate, and carbonate salts by young college women. Journal of Nutrition 48, 443–452.CrossRefGoogle ScholarPubMed
Rassin, D. K., Sturman, J. A. & Gaull, G. E. (1978) Taurine and other free amino acids in milk of man and other mammals. Early Human Development 2, 1–13.CrossRefGoogle ScholarPubMed
Robles, R. (1984) Serum and urine amino acid patterns during the first month of life for small-to-date infants. Biology of the Neonate 45, 209–217.CrossRefGoogle Scholar
Robles-Valdes, C., McGarry, J. D. & Foster, D. W. (1976) Maternal-fetal carnitine relationships and neonatal ketosis in the rat. Journal of Biological Chemistry 251, 6007–6012.CrossRefGoogle ScholarPubMed
Smart, J. L., Massey, R. F., Nash, S. C. & Tonkiss, J. (1987) Effects of early-life undernutrition in artificially-reared rats: subsequent body and organ growth. British Journal of Nutrition 58, 245–255.CrossRefGoogle ScholarPubMed
Smart, J. L., Stephens, D. N., Tonkiss, J., Auestad, N. S. & Edmond, J. (1984) Growth and development of rats artificially-reared on different milk substitutes. British Journal of Nutrition 52, 227–237.CrossRefGoogle ScholarPubMed
Smith, S., Watts, R. & Dils, R. (1968) Quantitative gas-liquid chromatographic analysis of rodent milk triglycerides. Journal of Lipid Research 9, 52–57.CrossRefGoogle ScholarPubMed
Sonnenberg, N., Bergstrom, J. D., Ha, Y. H. & Edmond, J. (1982) Metabolism in the artificially-reared rat pup; effect of an atypical rat milk substitute. Journal of Nutrition 112, 1506–1514.CrossRefGoogle ScholarPubMed
Spray, C. M. (1950) A study of some aspects of reproduction by means of chemical analysis. British Journal of Nutrition 4, 354–360.CrossRefGoogle ScholarPubMed
Thoman, E. B. & Arnold, W. J. (1967) Effects of incubator rearing with social deprivation on maternal behaviour in rats. Journal of Comparative and Physiological Psychology 65, 441–446.CrossRefGoogle Scholar
Tonkiss, J., Smart, J. L., Auestad, N. S. & Edmond, J. (1985) Type of milk substitute influences growth of the gastrointestinal tract in artificially-reared rat pups. Journal of Pediatric Gastroenterology and Nutrition 4, 818–825.Google ScholarPubMed
Tonkiss, J., Smart, J. L. & Massey, R. F. (1987) Growth and development of rats artificially-reared on rats' milk/milk substitute combinations. British Journal of Nutrition 57, 3–11.CrossRefGoogle ScholarPubMed
Vernon, R. G. & Walker, D. G. (1972)Glucose metabolism in the developing rat: studies in vivo. Biochemical Journal 127, 521–529.CrossRefGoogle ScholarPubMed
Webber, R. J. & Edmond, J. (1977) Utilization of L(+)-3-hydroxybutyrate, D-(-)-3-hydroxybutyrate, acetoacetate and glucose for respiration and lipid synthesis in the 18-day-old rat. Journal of Biological Chemistry 252,5222–5226.CrossRefGoogle ScholarPubMed
Webber, R. J. & Edmond, J. (1979) The in vivo utilization of acetoacetate, D(-)-3-hydroxybutyrate, and glucose for lipid synthesis in brain in the 18-day-old rat. Journal of Biological Chemistry 254, 3912–3920.CrossRefGoogle ScholarPubMed
West, D. B., Diaz, J. & Woods, S. C. (1982) Infant gastrostomy and chronic formula infusion as a technique to overfeed and accelerate weight gain of neonatal rats. Journal of Nutrition 112, 1339–1343.CrossRefGoogle ScholarPubMed
Williamson, D. H. (1982) The production and utilization of ketone bodies in the neonate. In The Biochemical Development of the Fetus and the Neonate pp. 621–650 [Jones, C. T., editor]. New York: Elsevier Biochemical.Google Scholar
Williamson, D. H., Bates, M. W., Page, M. A. & Krebs, H. A. (1971) Activities of enzymes involved in acetoacetate utilization in adult and mammalian tissues. Biochemical Journal 121, 41–47.CrossRefGoogle ScholarPubMed
Woodward, D. R. & Messer, M. (1976) Chemical composition of rat casein. Comparative Biochemistry and Physiology 55, 141–143.Google ScholarPubMed
Yeh, K. Y. (1983) Small intestine of artificially-reared rat pups: weight gain and changes in alkaline phosphatase, lactase and sucrase activities during development. Journal of Nutrition 113, 1489–1495.CrossRefGoogle ScholarPubMed
Yeh, Y. Y. (1980) Partition of ketone bodies into cholesterol and fatty acids in vivo in different brain regions of developing rats. Lipids 15, 904–907.CrossRefGoogle Scholar