Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-29T12:40:01.375Z Has data issue: false hasContentIssue false
  • English
  • Français

Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs

Published online by Cambridge University Press:  09 March 2007

M. A. Razzaque ,
J. H. Topps ,
R. N. B. Kay  and
J. M. Brockway
M. A. Razzaque
Affiliation:
School of Agriculture, 581 King Street, Aberdeen AB9 1 UD
J. H. Topps
Affiliation:
School of Agriculture, 581 King Street, Aberdeen AB9 1 UD
R. N. B. Kay
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
J. M. Brockway
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. A rumen bacterial culture containing specifically labelled nucleic acids was prepared using [8-14C]adenine.

2. The labelled preparation was given in a liquid diet to two preruminant lambs and via a rumen tube to two ruminant lambs. The radioactivity excreted in exhaled gases, faeces and urine and that incorporated into tissues was determined.

3 The preruminant lambs absorbed 58.3% of the total radioactivity measured after 24 h and the ruminant lambs 66.6% of the total activity measured after 48 h.

4. Of the total radioactivity absorbed the preruminant lambs exhaled 38%, excreted 34% in urine and retained 29% in tissues. The corresponding values for the ruminant lambs were 12,41 and 47% respectively.

5. There was a close relationship between total nucleic acid content and radioactivity per g of tissues of both preruminant and ruminant lambs.

6. Of the radioactivity in the urine, the ruminant and one preruminant lamb excreted most in the fraction containing allantoin, while the other lamb excreted most activity in the uric acid fraction.

7. The salvaging of the breakdown products of bacterial nucleic acids to make tissue nucleic acids appears to be an important synthesis in preruminant and ruminant lambs and of the likely precursors the purine base may be more important than the nucleoside.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 45 , Issue 3 , May 1981 , pp. 517 - 527
Copyright
Copyright © The Nutrition Society 1981

References

REFERENCES

Bamard, E. A. (1969), Nature, Land. 221, 340.Google Scholar
Baumgardt, B. R., Taylor, M. W. & Cason, J. L. (1962), J. Dairy Sci. 45, 62.CrossRefGoogle Scholar
Brockway, J. M., McDonald, J. D. & Pullar, J. D. (1977), A. Rep. Rowett Res. Inst. 33, 106.Google Scholar
Burton, K. (1956), Biochem. J. 62, 315.CrossRefGoogle Scholar
Coelho da Silva, J. F., Seely, R. C., Thomson, D. J., Beever, D. E. & Amstrong, D. G. (1972), Br. J. Nutr. 28, 43.Google Scholar
Condon, R. J., Hall, G. & Hatfield, E. E. (1970), J. Anim. Sci. 31, 1037.Google Scholar
Ellis, W. C. & Bleichner, K. L. (1969), J. Anim. Sci. 29, 157.Google Scholar
Ellis, W. C. & Pfander, W. H. (1965), Nature, Lond. 205, 974.CrossRefGoogle Scholar
Jackson, T. C., Schelling, G. T., Mitchell, G. E. & Tucker, R. E. (1976), J. Anim. Sci. 43, 325 Abstr.Google Scholar
Jurtshuk, P., Doetsch, R. N. & Shaw, J. C. (1958), J. Dairy Sci. 41, 190.CrossRefGoogle Scholar
McAllan, A. B. (1980), Br. J. Nutr. 44, 99.Google Scholar
Munro, H. N. & Fleck, A. (1967), Meth. Biochem. Analysis 14, 113.Google Scholar
Razzaque, M. A. (1973), Synthesis and metabolism of nucleic acids and related compounds in sheep and red deer. PhD Thesis, University of Aberdeen.Google Scholar
Razzaque, M. A. & Topps, J. H. (1978), J. Sci. Fd Agric. 29, 935.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1970), Br. J. Nutr. 24, 545.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1971), Br. J. Nurr. 24, 181.Google Scholar
Smith, R. C. & Mathur, C. F. (1973), Can. J. Microbiol. 19, 591.CrossRefGoogle Scholar
Smith, R. C., Mathur, N. M. & Hawkins, G. E. (1974), Br. J. Nutr. 32, 529.Google Scholar
Topps, J. H. & Elliott, R. C. (1965), Nature, Lond. 205, 498.CrossRefGoogle Scholar
Zvilna, R., Vitols, M., Novak, F. & Dienstbier, Z. (1975), Folia Microbiol. 24, 46.Google Scholar