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Metabolic responses to isoenergetic meals containing different proportions of carbohydrate and fat

Published online by Cambridge University Press:  09 March 2007

Helena A. Whitley ,
Sandy M. Humphreys ,
Jaswinder S. Samra ,
Iain T. Campbell ,
Donald P. M. Maclaren ,
Tom Reilly  and
Keith N. Frayn
Helena A. Whitley
Affiliation:
Oxford Lipid Metabolism Group, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE
Sandy M. Humphreys
Affiliation:
Oxford Lipid Metabolism Group, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE
Jaswinder S. Samra
Affiliation:
Oxford Lipid Metabolism Group, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE
Iain T. Campbell
Affiliation:
University Department of Anaesthesia, Withington Hospital, Nell Lane, Manchester M20 8LR
Donald P. M. Maclaren
Affiliation:
Centre for Sport and Exercise Sciences, Liverpool John Moores University, Mountford Building, Byrom Street, Liverpool L3 3AF
Tom Reilly
Affiliation:
Centre for Sport and Exercise Sciences, Liverpool John Moores University, Mountford Building, Byrom Street, Liverpool L3 3AF
Keith N. Frayn
Affiliation:
Oxford Lipid Metabolism Group, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE
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Abstract

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The purpose of the present study was to investigate the interrelationship between carbohydrate and fat metabolism at rest after isoenergetic meals of varying proportions of carbohydrate and fat. Eight physically-active subjects (BMI 18·1–23·4 kg/m2) were studied at rest on three occasions after an overnight fast. In a balanced design they were given meals containing carbohydrate, protein and fat in the following amounts respectively (g/70 kg body weight): meal 1 121,16,48; meal 2 70,16,70; meal 3 50, 14, 80. All meals were isoenergetic, containing 4·0 MJ/70 kg body weight, and were of similar appearance. In addition, on a fourth occasion five of the eight subjects consumed meal 4 (g/70 kg body weight): Carbohydrate 0, protein 0, fat 108. Blood samples were taken before eating the meal and at intervals following the meal to determine metabolic and hormonal responses. Energy expenditure and substrate oxidation were measured by indirect calorimetry and balance was calculated over the 5 h postprandial period. The incremental areas under the time curves for fat oxidation were greatest after meals 3 and 4 (P<0·05), whereas incremental areas under thecarbohydrate oxidation v. time curves were relatively reduced after these two meals (P<0·05). This was accompanied by lesser suppression of plasma non-esterified fatty acid concentrations (P<0·001) and reduced plasma insulin concentrations (P<0·001) following these meals. Energy balance was almost identical after the three isoenergetic meals. In contrast, there was an inverse relationship between Carbohydrate and fat balance following these meals, with carbohydrate balance decreasing as carbohydrate intake decreased and fat balance increasing as fat intake increased. We conclude that there is a close interrelationship between carbohydrate and fat metabolism following isoenergetic meals in resting subjects.

Keywords

Isoenergetic mealsSubstrate balanceSubstrate oxidation
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 1 , July 1997 , pp. 15 - 26
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Abbott, W. G. H., Howard, B. V., Christin, L., Freymond, D., Lillioja, S., Boyce, V. L., Anderson, T. E., Bogardus, C. & Ravussin, E. (1988). Short term energy balance: relationship with protein, carbohydrate, and fat balances. American Journal of Physiology 255, E332–E337.Google ScholarPubMed
Åstrand, P.-O. & Ryhming, I. (1954). A nomogram for calculation of aerobic capacity (physical fitness) from pulse rate during submaximal work. Journal of Applied Physiology 7, 218–221.CrossRefGoogle Scholar
Bennett, C., Reed, G. W., Peters, J. C., Abumrad, N. N., Sun, M. & Hill, J. O. (1992). Short-term effects of dietary-fat ingestion on energy expenditure and nutrient balance. American Journal of Clinical Nutrition 55, 1071–1077.Google Scholar
Bratusch-Marrain, P. R., Waldhäusl, W. K., Gasic, S., Korn, A. & Nowotny, P. (1980). Oral glucose tolerance test: effect of different glucose loads on splanchnic carbohydrate and substrate metabolism in healthy man. Metabolism 29, 289–295.CrossRefGoogle ScholarPubMed
Castro, A., Scott, J. P., Grettie, D. P., Macfarlane, D. & Bailey, R. E. (1970). Plasma insulin and glucose responses of healthy subjects to varying glucose loads during three-hour oral glucose tolerance tests. Diabetes 19, 842–851.CrossRefGoogle ScholarPubMed
Coppack, S. W., Fisher, R. M., Gibbons, G. F., Humphreys, S. M., McDonough, M. J., Potts, J. L. & Frayn, K. N. (1990). Postprandial substrate deposition in human forearm and adipose tissues in vivo. Clinical Science 79, 339–348.CrossRefGoogle ScholarPubMed
Durnin, J. V. G. A. & Womersley, J. (1974). Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. British Journal of Nutrition 32, 77–97.CrossRefGoogle ScholarPubMed
Eckel, R. H. (1989). Lipoprotein lipase: a multifunctional enzyme relevant to common metabolic diseases. New England Journal of Medicine 320, 1060–1068.Google ScholarPubMed
Fielding, B. A., Callow, J., Owen, M., Samra, J. S., Matthews, D. R. & Frayn, K. N. (1996). Postprandial lipemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals. American Journal of Clinical Nutrition 63, 36–41.Google Scholar
Flatt, J. P., Ravussin, E., Acheson, K. J. & Jéquier, E. (1985). Effects of dietary fat on postprandial substrate oxidation and on carbohydrate and fat balance. Journal of Clinical Investigation 76, 1019–1024.CrossRefGoogle Scholar
Gibbons, G. F. (1990). Assembly and secretion of hepatic very-low-density lipoprotein. Biochemical Journal 268, 1–13.Google Scholar
Griffiths, A. J., Humphreys, S. M., Clark, M. L., Fielding, B. A. & Frayn, K. N. (1994). Immediate metabolic availability of dietary fat in combination with carbohydrate. American Journal of Clinical Nutrition 59, 53–59.CrossRefGoogle ScholarPubMed
Lithell, H., Boberg, J., Hellsing, K., Lundqvist, G. & Vessby, B. (1978). Lipoprotein-lipase activity in human skeletal muscle and adipose tissue in the fasting and the fed states. Atherosclerosis 30, 89–94.CrossRefGoogle ScholarPubMed
McGuire, E. A. H., Helderman, J. H., Tobin, J. D., Andres, R. & Berman, M. (1976). Effects of arterial versus venous sampling on analysis of glucose kinetics in man. Journal of Applied Physiology 41, 565–573.CrossRefGoogle ScholarPubMed
Murphy, M. C., Isherwood, S. G., Sethi, S., Gould, B. J., Wright, J. W., Knapper, J. A. & Williams, C. M. (1995). Postprandial lipid and hormone responses to meals of varying fat contents: modulatory role of lipoprotein lipase? European Journal of Clinical Nutrition 49, 579–588.Google ScholarPubMed
Nordenström, J., Neeser, G., Olivecrona, T. & Wahren, J. (1991). Effect of medium- and long-chain triglyceride infusion on lipoprotein and hepatic lipase in healthy subjects. European Journal of Clinical Investigation 21, 580–585.CrossRefGoogle ScholarPubMed
Peterson, J., Bihain, B. E., Bengtsson-Olivecrona, G., Decklebaum, R. J., Carpentier, Y. A. & Olivecrona, T. (1990). Fatty acid control of lipoprotein lipase: a link between energy metabolism and lipid transport. Proceedings of the National Academy of Sciences USA 87, 909–913.CrossRefGoogle ScholarPubMed
Piers, L. S., Soares, M. J., Makan, T. & Shetty, P. S. (1992). Thermic effect of a meal. 1. Methodology and variation in normal young adults. British Journal of Nutrition 67, 165–175.Google Scholar
Potts, J. L., Fisher, R. M., Humphreys, S. M., Coppack, S. W., Gibbons, G. F. & Frayn, K. N. (1991). Peripheral triacylglycerol extraction in the fasting and post-prandial states. Clinical Science 81, 621–626.CrossRefGoogle ScholarPubMed
Randle, P. J., Garland, P. B., Hales, C. N. & Newsholme, E. A. (1963). The glucose fatty-acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet i, 785–789.Google Scholar
Schutz, Y., Flatt, J. P. & Jéquier, E. (1989). Failure of dietary fat intake to promote fat oxidation: a factor favoring the development of obesity. American Journal of Clinical Nutrition 50, 307–314.CrossRefGoogle ScholarPubMed
Stubbs, R. J., Murgatroyd, P. R., Goldberg, G. R. & Prentice, A. M. (1993). Carbohydrate balance and the regulation of day-to-day food intake in humans. American Journal of Clinical Nutrition 57, 897–903.CrossRefGoogle ScholarPubMed
Verboeket-van de Venne, W. P. H. G., Westerterp, K. R. & ten Hoor, F. (1994). Substrate utilization in man: effects of dietary fat and carbohydrate. Metabolism 43, 152–156.CrossRefGoogle ScholarPubMed
Warnick, G. R. & Albers, J. J. (1978). A comprehensive evaluation of the heparin-manganese precipitation procedure for estimating high density lipoprotein cholesterol. Journal of Lipid Research 19, 65–76.Google Scholar