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Magnesium deficiency improves glucose homeostasis in the rat: studies in vivo and in isolated islets in vitro

Published online by Cambridge University Press:  09 March 2007

Marise A. B. Reis ,
Márcia Q. Latorraca ,
Everardo M. Carneiro ,
Antonio C. Boschero ,
Mário J. A. Saad ,
Lício A. Velloso  and
Felix G. R. Reyes
Marise A. B. Reis
Affiliation:
Departamento de Fisiologia e Biofisica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Márcia Q. Latorraca
Affiliation:
Departamento de Nutrição e Dietética, Faculdade de Enfermagem e Nutrição (FEN), Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
Everardo M. Carneiro
Affiliation:
Departamento de Fisiologia e Biofisica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Antonio C. Boschero
Affiliation:
Departamento de Fisiologia e Biofisica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Mário J. A. Saad
Affiliation:
Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Lício A. Velloso
Affiliation:
Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Felix G. R. Reyes*
Affiliation:
Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
*
*Corresponding author: Dr Felix G. R. Reyes, fax +55 19 3289 2832, email [email protected]
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Abstract

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The serum mineral levels, glucose disappearance rate (kg), total area under the glucose (ΔG) and insulin (ΔI) curves, and static insulin secretion were compared among rats fed a Mg-deficient diet for 6 (DF-6) or 11 (DF-11) weeks, and rats fed a control diet for the same periods (CO-6 and CO-11 groups). No change in glucose homeostasis was observed among DF-6, CO-6 and CO-11 rats. DF-11 rats showed an elevated kg and a reduced ΔG and ΔI. For evaluating the effect of supplementation, rats fed a control or Mg-deficient diet for 6 weeks were then fed a Mg- supplemented diet for 5 weeks (SCO and SDF groups respectively). The serum Mg levels in SDF rats were similar to those in CO-11 and SCO rats, but higher than in the DF-11 group. SDF rats showed similar kg, ΔG and ΔI compared with the CO-11 and SCO groups. However, a significantly lower kg and higher ΔG and ΔI were observed in SDF compared with DF-11 rats. Basal and 8.3 mmol glucose/l-stimulated insulin secretion by islets from DF-11 rats were higher than by islets from CO-11 rats. These results indicate that moderate Mg depletion for a long period may increase the secretion and sensitivity to insulin, while Mg supplementation in formerly Mg-deficient rats may prevent the increase in sensitivity and secretion of insulin.

Keywords

Magnesium deficiencyInsulin sensitivityGlucose homeostasisRats
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 5 , May 2001 , pp. 549 - 552
Copyright
Copyright © The Nutrition Society 2001

References

Curry, DL, Joy, RM, Holley, DC & Bennett, LL (1977) Magnesium modulation of glucose-induced insulin secretion by perfused rats pancreas. Endocrinology 101, 203–208.CrossRefGoogle Scholar
Elin, RJ (1994) Magnesium. The fifth but forgotten electrolyte. American Journal of Clinical Pathology 102, 616–622.CrossRefGoogle ScholarPubMed
Garber, A, Blackard, WG, Feinglos, M, Haffner, S, Lasagna, L, Maryniuk, M, Rivlin, R & Sussman, KE (1995) Consensus statement. Magnesium supplementation in the treatment of diabetes. Diabetes Care 18(Suppl. 1), 83–85.Google Scholar
Ishizuka, J, Bold, RJ, Townsend CM, Jr & Thompson, JC (1994) In vitro relationship between magnesium and insulin secretion. Magnesium Research 7, 17–22.Google ScholarPubMed
Kahil, ME, Simons, EL & Brown, H (1968) Magnesium deficiency and sugar transport in muscle. Diabetes 17, 673–678.CrossRefGoogle ScholarPubMed
Kandeel, FR, Balon, E, Scott, S & Nadler, JL (1996) Magnesium deficiency and glucose metabolism in rat adipocytes. Metabolism 45, 838–843.CrossRefGoogle ScholarPubMed
Latorraca, MQ, Carneiro, EM, Boschero, AC & Mello, MAR (1998) Protein deficiency during pregnancy and lactation impairs glucose-induced insulin secretion but increases the sensitivity to insulin in weaned rats. British Journal of Nutrition 80, 291–297.CrossRefGoogle ScholarPubMed
Legrand, C, Okitolonda, W, Pottier, AM, Lederer, J & Henquin, JC (1987) Glucose homeostasis in magnesium-deficient rats. Metabolism 36, 160–164.CrossRefGoogle ScholarPubMed
Lowney, P, Hannon, TS & Baron, AD (1995) Magnesium deficiency enhances basal glucose disposal in the rat. American Journal of Physiology 268, E925–E931.Google ScholarPubMed
Matthews, JNS, Altman, DG, Campbell, MJ & Royston, P (1990) Analysis of serial measurements in medical research. British Medical Journal 27, 230–235.CrossRefGoogle Scholar
Reis, MAB, Reyes, FGR, Saad, MJA & Velloso, LA (2000) Magnesium deficiency modulates the insulin signaling pathway in liver but not muscle of rats. Journal of Nutrition 130, 133–138.CrossRefGoogle Scholar
Scott, AM, Atwater, I & Rojas, E (1981) A method for the simultaneous measurement of insulin released and β-cell membrane potential in single mouse islets of Langerhans. Diabetologia 21, 407–475.CrossRefGoogle Scholar
Suaréz, A, Pulido, N, Casla, A, Casanova, B, Arrieta, FJ & Rovira, A (1995) Impaired tyrosine-kinase activity of muscle insulin receptors from hypomagnesaemic rats. Diabetologia 35, 1262–1270.CrossRefGoogle Scholar
Trinder, P (1969) Determinations of blood glucose using on oxidase-peroxidase system with a non-carcinogenic chromogen. Journal of Clinical Pathology 22, 158-161.CrossRefGoogle ScholarPubMed