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Lysine requirements and whole-body protein turnover in growing pigs

Published online by Cambridge University Press:  09 March 2007

D. N. Salter ,
A. I. Montgomery ,
Anna Hudson ,
D. B. Quelch  and
Rosemary J. Elliott
D. N. Salter
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
A. I. Montgomery
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
Anna Hudson
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
D. B. Quelch
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
Rosemary J. Elliott
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
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Abstract

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The influence on protein accretion and whole-body protein turnover of changing dietary protein quality while maintaining constant energy intake was studied by varying the degree of lysine supplementation of a lysine-deficient barley-based diet given to growing pigs. Measurements of nitrogen metabolism and whole-body protein turnover, using both classical and 15N end-product methods following a single dose of lsqb;15N]glycine, were made in 49-kg male pigs given diets containing 109 g lysine-deficient protein/kg supplemented to make them (1) 'deficient', (2) 'adequate' and (3) 'in excess' with respect to lysine. The 15N dose and protein intake values used to calculate amino N flux from the cumulative urinary excretion of 15N in urea and ammonia were corrected respectively for apparent digestibilities of [15N]glycine and total N determined in a separate experiment in pigs fitted with simple ileal cannulas. N retention and biological value were significantly increased by lysine supplementation of the deficient diet to the 'adequate' level, but were not further increased by the higher level of supplementation. Rates of growth paralleled these changes. The poorer biological value of the unsupplemented diet 1 was shown also in a significantly higher excretion of urea N compared with diets 2 and 3. N digestibility was not markedly influenced by the level of lysine supplementation. Both whole-body protein synthesis and degradation increased markedly on 'adequate' supplementation of the diet with lysine, but did not increase further with an excess of lysine. It is concluded that the increase in protein accretion rate observed on supplementation of the diet with lysine was due to a greater increase in the rate of protein synthesis than of degradation, rather than a decrease in degradation rate.

Keywords

GrowthLysine requirementProtein turnoverPig
Type
Protein Nutrition and Metabolism
Information
British Journal of Nutrition , Volume 63 , Issue 3 , May 1990 , pp. 503 - 513
Copyright
Copyright © The Nutrition Society 1990

References

REFERENCES

Agricultural Research Council (1981). The Nutrient Requirements of Pigs, pp. 67–124. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Clugston, G. A. & Garlick, P. J. (1982). The response of protein and energy metabolism to food intake in lean and obese man. Human Nutrition: Clinical Nutrition 36C, 57–70.Google ScholarPubMed
Conway, E. J., (1957) Microdiffusion Analysis and Volumetric Error, pp. 98–133. London: Crosby Lockwood & Son Ltd.Google Scholar
Everson, W. V., Flaim, K. E., Susco, D. M., Kimball, S. R. & Jefferson, L. S. (1989). Effect of amino-acid deprivation on initiation of protein synthesis in rat hepatocytes. American Journal of Physiology 256, C18–C27.CrossRefGoogle ScholarPubMed
Fern, E. B., Garlick, P. J. & Waterlow, J. C. (1985). Apparent compartmentation of body nitrogen in one human subjcct: its consequences in measuring the rate of whole-body protein synthesis with 15N. Clinical Science 68, 271–282.Google Scholar
Ferrari, A. (1960). Nitrogen determination by a continuous digestion and analysis system. Annals of the New York Academy of Sciences 87, 792–800.CrossRefGoogle ScholarPubMed
Fuller, M. F., Cadenhead, A., Mollison, G. & Seve, B. (1987 a). Effects of the amount and quality of dietary protein on nitrogen metabolism and heat production in growing pigs. British Journal of Nutrition 58, 277–285.CrossRefGoogle ScholarPubMed
Fuller, M. F., Reeds, P. J., Cadenhead, A., Seve, B. & Preston, T. (1987 b). Effects of the amount and quality of dietary protein on nitrogen metabolism and protein turnover of pigs. British Journal of Nutrition 58, 287–300.CrossRefGoogle ScholarPubMed
Garlick, P. J., McNurlan, M. A., McHardy, K. C., Reeds, P. J., Preedy, V. R. & Clugston, G. A. (1986). Protein turnover and food intake. In Proceedings of the 13th International Congress of Nutrition, 1985, pp. 374–377 [Taylor, T. G. and Jenkins, N. K., editors]. London, Paris: John Libbey.Google Scholar
Jackson, A. A. & Golden, M. H. N. (1981). Interrelationships of amino acid pools and protein turnovcr. In Nitrogen Metabolism in Man, pp. 361–373 [Watcrlow, J. C. and Stephen, J. M. L., editors]. London: Applied Science Publishers.Google Scholar
Jagus, R., Anderson, W. F. & Safer, B. (1981). The regulation of initiation of mammalian protein synthesis. Progress in Nucleic Acids Research and Molecular Biology 25, 127–183.CrossRefGoogle ScholarPubMed
Millward, D. J., Garlick, P. J. & Reeds, P. J. (1976). The energy cost of growth. Proceedings of the Nutrition Society 35, 339–349.CrossRefGoogle ScholarPubMed
Nishizawa, N., Shimbo, M., Hareyama, S. & Funabiki, R. (1977). Fractional catabolic rates of myosin and actin estimated by urinary excretion of N'-methylhistidine: the effect of dietary protein level on catabolic rates under conditions of restricted food intake. British Journal of Nutrition 37, 345–353.CrossRefGoogle Scholar
Omstedt, P. T., Kihlberg, R., Tingvall, P. & Shenkin, A. (1978). Effect of dietary protein on urinary excretion of 3-methylhistidine in the rat. Journal of Nutrition 108, 1877–1882.Google Scholar
Read, W. W. C., Harrison, R. A. & Halliday, D. (1982). A resin-based method for the preparation of molecular nitrogen for 15N analysis from urinary and plasma components. Analytical Biochemisty 123, 249–254.Google Scholar
Reeds, P. J., Cadenhead, A., Fuller, M. F., Lobley, G. E. & McDonald, J. D. (1980). Protein turnover in growing pigs. Effects of age and food intake. British Journul of Nutrition 43, 445–455.CrossRefGoogle ScholarPubMed
Reeds, P. J. & Fuller, M. F. (1983). Nutrient intake and protein turnover. Proceedings of the Nutrition Society 42, 463–471.Google Scholar
Salter, D. N., Montgomery, A. I., Hudson, A., Quelch, D. B. & Elliott, R. J. (1988). The influence on whole-body protein turnover and nitrogen balance of lysine deficiency or excess in barley-based diets for growing pigs. European Association for Animal Production Publication no. 35. Proceedings of the 5th International Symposium on Protein MetaboliNutrition, Rostock, pp.34–35. Rostock, GDR: Wilhelm-Pieck-University of Rostock.Google Scholar
Salter, D. N. & Smith, R. H. (1977). Digestibilities of nitrogen compounds in rumen bacteria and in other components of digesta in the small intestine of the young steer. British Journal of Nutrition 38, 207–216.CrossRefGoogle ScholarPubMed
Smith, R. H. (1962). Net exchange of certain inorganic ions and water in the alimentary tract of the milk-fed calf. Biochemical Journal 83, 151–163.Google Scholar
Van soest, P. J. (1973). Collaborative study of acid-detergent fiber and lignin. Journal qf the Association of Official Analytical Chemists 56, 781–784.Google Scholar
Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978 a). Protein Turnover in Mammalian Tissues and in the Whole Body, pp. 251–299. Amsterdam, New York, London: North Holland Publishing Company.Google Scholar
Waterlow, J. C., Golden, M. H. N. & Garlick, P. J. (1978 b). Protein turnover in man measured with 15N: comparison of end products and dose regimes. American Journal of Physiology 235, El65–E174.Google Scholar
Waterlow, J. C. & Jackson, A. A. (1981). Nutrition and protein turnover in man. British Medical Bulletin 37, 5–10.CrossRefGoogle ScholarPubMed
Young, V. R., Munro, H. N., Mathers, D. E. & Bier, D. M. (1983). In New Aspects of Clinica1 Nutrition, pp. 43–73 [Kleinberger, G. and Deutsch, E. editors]. Basel: Karger.Google Scholar