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Lycopene supplementation attenuated xanthine oxidase and myeloperoxidase activities in skeletal muscle tissues of rats after exhaustive exercise

Published online by Cambridge University Press:  08 March 2007

Chieh-Chung Liu ,
Chi-Chang Huang ,
Wan-Teng Lin ,
Chin-Cheng Hsieh ,
Shih-Yi Huang ,
Su-Jiun Lin  and
Suh-Ching Yang
Chieh-Chung Liu
Affiliation:
Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan
Chi-Chang Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
Wan-Teng Lin
Affiliation:
Department of Nutrition and Food Sciences, Fu-Jen Catholic University, Taipei, Taiwan De Lin Institute of Technology, Taipei, Taiwan
Chin-Cheng Hsieh
Affiliation:
Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan
Shih-Yi Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
Su-Jiun Lin
Affiliation:
Graduate Institute of Biology and Environment Science, School of Cellular and Molecular Biology, University of New Haven, CT 06516, USA
Suh-Ching Yang*
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
*
*Corresponding author: Dr Suh-Ching Yang, fax +886 2 2737 3112, email [email protected]
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Abstract

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Strenuous exercise is known to induce oxidative stress leading to the generation of free radicals. The purpose of the present study was to investigate the effects of lycopene, an antioxidant nutrient, at a relatively low dose (2·6 mg/kg per d) and a relatively high dose (7·8 mg/kg per d) on the antioxidant status of blood and skeletal muscle tissues in rats after exhaustive exercise. Rats were divided into six groups: sedentary control (C); sedentary control with low-dose lycopene (CLL); sedentary control with high-dose lycopene (CHL); exhaustive exercise (E); exhaustive exercise with low-dose lycopene (ELL); exhaustive exercise with high-dose lycopene (EHL). After 30 d, the rats in the three C groups were killed without exercise, but the rats in the three E groups were killed immediately after an exhaustive running test on a motorised treadmill. The results showed that xanthine oxidase (XO) activities of plasma and muscle, and muscular myeloperoxidase (MPO) activity in group E were significantly increased compared with group C. Compared with group E, the elevations of XO and MPO activities of muscle were significantly decreased in group EHL. The malondialdehyde concentrations of plasma and tissues in group E were significantly increased by 72 and 114 %, respectively, compared with those in group C. However, this phenomenon was prevented in rats of the ELL and EHL groups. There was no significant difference in the GSH concentrations of erythrocytes in each group; however, exhaustive exercise resulted in a significant decrease in the GSH content of muscle. In conclusion, these results suggested that lycopene protected muscle tissue from oxidative stress after exhaustive exercise.

Keywords

LycopeneXanthine oxidaseMyeloperoxidaseOxidative stressExhaustive exercise
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 4 , October 2005 , pp. 595 - 601
Copyright
Copyright © The Nutrition Society 2005

References

Atalay, M, Laaksonen, DE, Khanna, S, Kaliste-Korhonen, E, Hanninen, O & Sen, CK (2000) Vitamin E regulates changes in tissue antioxidants induced by fish oil and acute exercise. Med Sci Sports Exerc 32, 601–607.CrossRefGoogle ScholarPubMed
Banerjee, AK, Mandal, A, Chanda, D & Chakraborti, S (2003) Oxidant, antioxidant and physical exercise. Mol Cell Biochem 253, 307–312.CrossRefGoogle ScholarPubMed
Belcastro, AN, Arthur, GD, Albisser, TA & Raj, DA (1996) Heart, liver, and skeletal muscle myeloperoxidase activity during exercise. J Appl Physiol 80, 1331–1335.CrossRefGoogle ScholarPubMed
Bowers, WD Jr, Hubbard, RW, Leav, IDaum, R, Conlon, M, Hamlet, MP, Mager, M & Brandt, P (1978) Alterations of rat liver subsequent to heat overload. Arch Pathol Lab Med 102, 154–157.Google ScholarPubMed
Brooks, GA & White, TP (1978) Determination of metabolic and heart rate responses of rats to treadmill exercise. J Appl Physiol 45 1009–1015.CrossRefGoogle ScholarPubMed
Clarkson, PM & Thompson, HS (2000) Antioxidants: what role do they play in physical activity and health?. Am J Clin Nutr 72, 637S–646S.CrossRefGoogle ScholarPubMed
Di Mascio, P, Kaiser, S & Sies, H (1989) Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 274, 532–538.CrossRefGoogle ScholarPubMed
Fielding, RA, Manfredi, T, Ding, W, Fiatarone, MA, Evans, WJ & Cannon, JG (1993) Acute phase response in exercise III. Neutrophil and IL-1 beta accumulation in skeletal muscle. Am J Physiol 265, R166–R172.Google ScholarPubMed
Goto, H & Ito, A & Mikami, (1989) Effect of exercise on urate exerction. Nippon Seirigaku Zasshi 51, 208–220.Google Scholar
Gupta, SK, Trivedi, D, Srivastava, S, Joshi, S, Holder, N & Verma, SD (2003) Lycopene alternates oxidative stress induced experimental cataract development: an in vitro and in vivo study. Nutrition 19, 794–799.CrossRefGoogle Scholar
Hellsten-Westing, Y, Kaijser, L, Ekblom, B & Sjodin, B (1994) Exchange of purines in human liver and skeletal muscle with short-term exhaustive exercise. Am J Physiol 266, R81–R86.Google ScholarPubMed
Heunks, LMA, Vina, J, Van Herwaarden, CLA, Folgering, HTM, Gimeno, A & Dekhuijzen, PNR (1999) Xanthine oxidase is involved in exercise-induced oxidative stress in chronic obstructive pulmonary disease. Am J Physiol 277, R1697–R1704.Google ScholarPubMed
Husain, K (2003) Interaction of physical training and chronic nitroglycerin treatment on blood pressure, nitric oxide, and oxidants/antioxidants in the rat heart. Pharmacol Res 48, 253–261.CrossRefGoogle ScholarPubMed
Itoh, H, Ohkuwa, T, Yamazaki, Y, Shimoda, T, Wakayama, A, Tamura, S, Yamamoto, T, Sato, Y & Miyamura, M (2000) Vitamin E supplementation attenuates leakage of enzymes following 6 successive days of running training. Int J Sports Med 21, 369–374.CrossRefGoogle ScholarPubMed
Jain, CK, Agarwal, S & Rao, AV (1999) The effect of dietary lycopene on bioavailability, tissue distribution, in vivo antioxidant properties and colonic preneoplasia in rats. Nutr Res 19, 1383–1391.CrossRefGoogle Scholar
Jimenez, L, Lefevre, G, Richard, R, Couderc, R, Saint, George M, Duvallet, A & Rieu, M (2001) Oxidative stress in hemodialyzed patients during exhausting exercise. J Sports Med Phys Fitness 41, 513–520.Google ScholarPubMed
Khanna, S, Atalay, M, Laaksonen, DE, Gul, M, Roy, S & Sen, CK (1999) Alpha-lipoic acid supplementation: tissue glutathione homeostasis at rest and after exercise. J Appl Physiol 86, 1191–1196.CrossRefGoogle ScholarPubMed
Koyama, K, Kaya, M, Ishigaki, T, Tsujita, J, Hori, S, Seino, T & Kasugai, A (1999) Role of xanthine oxidase in delayed lipid peroxidation in rat liver induced by acute exhausting exercise. Eur J Appl Physiol Occup Physiol 80, 28–33.CrossRefGoogle ScholarPubMed
Kumar, CT, Reddy, VK, Prasad, M, Thyagaraju, K & Reddanna, P (1992) Dietary supplementation of vitamin E protects heart tissue from exercise-induced oxidant stress. Mol Cell Biochem 111, 109–115.CrossRefGoogle ScholarPubMed
Leal, M, Shimada, A, Ruiz, F & Gonzalez de Mejia, E (1999) Effect of lycopene on lipid peroxidation and glutathione-dependent enzymes induced by T-2 toxin in vivo. Toxicol Lett 109, 1–10.CrossRefGoogle ScholarPubMed
Lederman, JD, Overton, KM, Hofmann, NE, Moore, BJ, Thornton, J & Erdman, JW (1998) Ferrets ( Mustela putoius furo ) inefficiently convert β-carotene to vitamin A. J Nutr 128, 271–279.Google ScholarPubMed
Lowry, OH, Rosebrough, NJ, Lewis, Farr A & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265–275.CrossRefGoogle ScholarPubMed
McCord, JM (1985) Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 312 159–163.Google ScholarPubMed
Marquez, R, Santangelo, G, Sastre, J, Goldschmidt, P, Luyckx, J, Pallardo, FV & Vina, J (2001) Cyanoside chloride and chromocarbe diethylamine are more effective than vitamin C against exercise-induced oxidative stress. Pharmacol Toxicol 89 255–258.CrossRefGoogle ScholarPubMed
Mastaloudis, A, Morrow, JD, Hopkins, DW, Devaraj, S & Traber, MG (2004) Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radic Biol Med 36, 1329–1341.CrossRefGoogle Scholar
Morozov, VI, Pryatkin, SA, Kalinski, MI & Rogozkin, VA (2003) Effect of exercise to exhaustion on myeloperoxidase and lysozyme release from blood neutrophils. Eur J Appl Physiol 89, 257–262.CrossRefGoogle ScholarPubMed
Mullane, KM, Kraemer, R & Smith, B (1985) Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium. J Pharmacol Methods 14, 157–167.CrossRefGoogle ScholarPubMed
Pan, H, Jiang, X, Wan, L, Na, L & Wang, J (2004) Experimental studies of lycopene in inhibiting tumor growth in S180-bearing mice. Wei Sheng Yan Jiu 33, 456–457.Google ScholarPubMed
Radak, Z, Asano, K, Inoue, M, Kizaki, T, Oh-Ishi, S, Suzuki, K, Taniguchi, N & Ohno, H (1995) Superoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. J Appl Physiol 79, 129–135.CrossRefGoogle ScholarPubMed
Reddy, KV, Kumar, TC, Prasad, M & Reddanna, P (1998) Pulmonary lipid peroxidation and antioxidant defenses during exhaustive physical exercise: the role of vitamin E and selenium. Nutrition 14, 448–451.CrossRefGoogle ScholarPubMed
Reifen, R, Nissenkorn, A, Matas, Z & Bujanover, Y (2004) 5-ASA and lycopene decrease the oxidative stress and inflammation induced by iron in rats with colitis. J Gastroenterol 39, 514–519.CrossRefGoogle ScholarPubMed
Schierwagen, C, Bylund-Fellenius, AC & Lundberg, C (1990) Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods 23, 179–186.CrossRefGoogle ScholarPubMed
Suzuki, K, Sato, H, Kikuchi, T, Abe, TNakaji, S, Sugawara, K, Totsuka, M, Sato, K & Yamaya, K (1996) Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise. J Appl Physiol 81, 1213–1222.CrossRefGoogle ScholarPubMed
Tidball, JG (2005) Inflammatory processes in muscle injury and repair. Am J Physiol 288, R345–R353.Google ScholarPubMed
Vina, J, Gimeno, A, Sastre, J, Desco, C, Asensi, M, Pallardo, FVCuesta, A, Ferrero, JA, Terada, LS & Repine, JE (2000) Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life 49, 539–544.CrossRefGoogle ScholarPubMed
Westerfeld, WW, Richert, DA & Higgins, ES (1959) Further studies with xanthine oxidase inhibitors. J Biol Chem 234, 1897–1900.CrossRefGoogle ScholarPubMed
Westing, YH, Ekblom, B & Sjodin, B (1989) The metabolic relation between hypoxanthine and uric acid in man following maximal short-distance running. Acta Physiol Scand 137, 341–345.CrossRefGoogle ScholarPubMed
Wu, HJ, Chen, KT, Shee, BW, Chang, HC, Huang, YJ & Yang, RS (2004) Effects of 24 h ultra-marathon on biochemical and hematological parameters. World J Gastroenterol 10, 2711–2714.CrossRefGoogle ScholarPubMed
Yamada, M, Suzuki, K, Kudo, S, Totsuka, M, Simoyama, T, Nakaji, S & Sugawara, K (2000) Effect of exhaustive exercise on human neutrophils in athletes. Luminescence 15, 15–20.3.0.CO;2-O>CrossRefGoogle ScholarPubMed
Zajac, A, Waskiewicz, Z & Pilis, W (2001) Anaerobic power, creatine kinase activity, lactate concentration, and acid-base equilibrium changes following bouts of exhaustive strength exercises. J Strength Cond Res 15, 357–361.Google ScholarPubMed