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Low-protein diet prevents tissue lipoprotein lipase activity increase in growing rats

Published online by Cambridge University Press:  09 March 2007

A. Boualga ,
M. Bouchenak  and
J. Belleville
A. Boualga
Affiliation:
Laboratoire de Physiologie Animale et de la Nutrition, Facultè des Sciences, Universitè d'Oran, Es-Sènia, 31 000 Oran, Algeria
M. Bouchenak
Affiliation:
Laboratoire de Physiologie Animale et de la Nutrition, Facultè des Sciences, Universitè d'Oran, Es-Sènia, 31 000 Oran, Algeria
J. Belleville*
Affiliation:
Unitè de Nutrition Cellulaire et Mètabolique, Facultè des Sciences Mirande, Universitè de Bourgogne, BP 400, 21011 Dijon Cedex, France
*
*Corresponding author: Dr J. Belleville, fax +33 3 80 39 63 30, email [email protected]
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Abstract

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The time course of changes in tissue lipolytic activities was studied in young rats during the consumption of a low-protein diet containing 50 g protein/kg (40 g wheat gluten +10 g casein/kg) for 28 d followed by balanced refeeding with 200 g protein/kg (160 g wheat gluten +40 g casein/kg) for 28 d. Lipoprotein lipase (LPL) activities were compared with the values of a control group fed a balanced diet containing 200 g protein/kg for 56 d. At the end of protein malnutrition period, the epididymal fat tissue LPL activity represented 36 %, and that of heart and gastrocnemius was 44 %, of those of the control group. These differences were accompanied by lower serum- and VLDL-triacylglycerols (TAG), respectively 47·6 % and 31 % of the control group values, probably resulting from reduced synthesis of VLDL-apolipoproteins (29 % of control group values), concomitant with liver lipid accumulation (4·8-fold) and little lipid storage in epididymal fat tissue. At day 2 of refeeding, there was no significant difference in liver and epididymal fat tissue LPL activities between experimental and control rats. At the end of the refeeding period, LPL activity of epididymal fat and liver lipolytic activity had increased and became similar to control group values. The consumption of a low-protein diet prevented the increase in extrahepatic LPL activities as observed in the control group. The alterations in LPL activity suggest that a low-protein diet limits lipid storage in adipose tissue due to reduced serum VLDL-TAG availability.

Keywords

Lipoprotein lipaseProtein malnutritionBalanced refeedingRat
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 5 , November 2000 , pp. 663 - 671
Copyright
Copyright © The Nutrition Society 2000

References

Agbedana, EO (1980) Changes in serum post heparin and tissue lipoprotein lipase activities in protein malnourished rats.Nutrition Reports International 32, 157–165.Google Scholar
Barker, DJP (1995) Fetal origins of coronary heart disease.British Medical Journal 311, 544–550.CrossRefGoogle ScholarPubMed
Barker, DJP, Gluckman, PD, Godfrey, KM, Harding, JE, Owens, JA and Robinson, JS (1993) Fetal nutrition and cardiovascular disease in adult life.Lancet 341, 938–941.CrossRefGoogle ScholarPubMed
Ben-Zeev, O, Lusis, AJ, Laboeuf, RC, Nikazy, J and Schotz, MC (1983) Evidence for independent genetic regulation of heart and adipose lipoprotein lipase activity.Journal of Biological Chemistry 258, 13632–13640.CrossRefGoogle ScholarPubMed
Borensztajn, J, Samols, DR and Rubenstein, AH (1972) Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue.American Journal of Physiology 223, 1271–1275.CrossRefGoogle ScholarPubMed
Brunzell, JD,(1989) Familial lipoprotein lipase deficiency and other causes of chylomicronemia syndrome In The Metabolic Basis of Inherited Disease pp.1165–1180[Scriver, CRBaudet, ALSly, WS and Valle, D, editors]. New York, NY: Mc Graw Hill.Google Scholar
Council of European Communities(1986) Directives of the Council concerning the animal protection for the use of living animals in scientific investigations.Official Journal of European Communities L358, 1–28.Google Scholar
Doolittle, MH, Ben-Zeev, O, Elovson, J, Martin, D and Kirchgessner, TG (1990) The response of lipoprotein lipase to feeding and fasting.Journal of Biological Chemistry 265, 4570–4578.CrossRefGoogle ScholarPubMed
Duncan, DB (1955) Multiple range and multiple F-tests.Biometrics 15, 1–42.CrossRefGoogle Scholar
Eckel, RH (1989) Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases.New England Journal of Medicine 320, 1060–1068.Google ScholarPubMed
Eisenberg, S (1984) High density lipoprotein metabolism.Journal of Lipid Research 25, 1017–1058.CrossRefGoogle ScholarPubMed
Faergeman, O and Havel, RJ (1975) Metabolism of cholesteryl esters of rat very low density lipoproteins.Journal of Clinical Investigation 55, 1210–1218.CrossRefGoogle ScholarPubMed
Fidge, NH and Poulis, P (1975) Studies on the metabolism of rat serum very low density lipoprotein.Journal of Lipid Research 16, 367–378.CrossRefGoogle Scholar
Flores, H, Pak, N, Macioni, A and Monckeberg, F (1970) Lipid transport in kwashiorkor.British Journal of Nutrition 24, 1005–1011.CrossRefGoogle ScholarPubMed
Flores, H, Sierralta, W and Monckeberg, F (1970) Triglyceride transport in protein depleted rats.Journal of Nutrition 100, 375–379.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M and Sloane Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues.Journal of Biological Chemistry 26, 497–509.CrossRefGoogle Scholar
Ginsberg, HN (1991) Lipoprotein physiology in nondiabetic and diabetic states. Relationship to atherogenesis.Diabetes Care 14, 839–855.CrossRefGoogle ScholarPubMed
Goldberg, IJ, Scheraldi, CA, Yacoub, LK, Saxena, U and Bisgaier, CL (1990) Lipoprotein apoC-II activation of lipoprotein lipase.Journal of Biological Chemistry 265, 4266–4271.CrossRefGoogle ScholarPubMed
Hamosh, M, Clary, TR, Chernick, SS and Scow, RD (1970) Lipoprotein lipase activity of adipose and mammary tissue and plasma triglycerides in pregnant and lactating rats.Biochimica et Biophysica Acta 210, 473–482.CrossRefGoogle ScholarPubMed
Hills, MJ and Mukherjee, KD (1988) Assay for triacylglycerol-lipase: A rapid thin layer chromatographic technique.Journal of Lipid Research 29, 1397–1399.CrossRefGoogle ScholarPubMed
Ikeda, Y, Atsuko, T and Yamamoto, A (1989) Purification and characterization of lipoprotein lipase and hepatic triglyceride lipase from human postheparin plasma: Production of monospecific antibody to the individual lipase.Biochimica et Biophysica Acta 1003, 254–269.CrossRefGoogle Scholar
Kadowaki, H, Patton, GM and Robins, SJ (1992) Metabolism of high density lipoprotein lipids by the rat liver: evidence for the participation of hepatic lipase in the uptake of cholesteryl esters.Journal of Lipid Research 33, 1689–1698.CrossRefGoogle Scholar
Kuusi, T, Kinnunen, PKS and Nikkila, EA (1979) Hepatic endothelial lipase antiserum influences rat plasma low and high density lipoprotein in vivo.FEBS Letters 104, 384–388.CrossRefGoogle ScholarPubMed
Kuusi, T, Nikkita, EA, Virtamen, J and Kinnunen, PK (1979) Localization of the heparin-releasable lipase in situ in the rat liver.Biochemistry Journal 181, 245–246.CrossRefGoogle ScholarPubMed
Lamri, MY, Meghelli-Bouchenak, M, Boualga, A, Belleville, J and Prost, J (1995) Rat plasma VLDL composition and concentration and hepatic lipase and lipoprotein lipase activities are impaired during two types of protein malnutrition and unaffected by balanced refeeding.Journal of Nutrition 125, 2425–2434.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL and Randall, RJ (1951) Protein measurement with the Folin phenol reagent.Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Lunn, PG and Austin, S (1983) Difference in nitrogen metabolism between protein deficiency and energy deficient rats with similarly restricted growth rates.Annals of Nutrition and Metabolism 27, 242–251.CrossRefGoogle ScholarPubMed
Meghelli-Bouchenak, M, Belleville, J and Boquillon, M (1989) Hepatic steatosis and serum very low density lipoproteins during two types of low protein diets followed by a balanced refeeding.Nutrition 5, 321–329.Google ScholarPubMed
Meghelli-Bouchenak, M, Belleville, J, Boquillon, M, Scherrer, B and Prost, J (1991) Removal rates of rat serum 3H-labeled very low lipoproteins after consumption of two different low protein diets.Nutrition Research 11, 265–275.Google Scholar
Meghelli-Bouchenak, M, Boquillon, M and Belleville, J (1989) Serum lipoproteins composition and amount during the consumption of different low protein diets followed by a balanced diet.Nutrition Reports International 39, 323–343.Google Scholar
Meghelli-Bouchenak, M, Boquillon, M and Belleville, J (1987) Time course of changes in rat serum apolipoproteins during the consumption of different low protein diets followed by a balanced diet.Journal of Nutrition 117, 641–649.CrossRefGoogle ScholarPubMed
Mohamed-Benkada, K, Belleville, J and Prost, J (1993) Comparative changes between pancreas and pancreatic juice digestive enzyme contents during nutritional rehabilitation following severe protein malnutrition in the rat.British Journal of Nutrition 69, 83–90.CrossRefGoogle ScholarPubMed
Okabe, T, Yorifuji, H, Murase, T and Takaku, F (1984) Pulmonary macrophage: a major source of lipoprotein lipase in the lung.Biochemical and Biophysical Research Communications 125, 273–278.CrossRefGoogle Scholar
Olivecrona, T and Bengtsson-Olivecrona, G (1990) Lipoprotein lipase and hepatic lipase.Current Opinion in Lipidology 1, 222–230.CrossRefGoogle Scholar
Onitri, AC and Boyo, AE (1975) Serum lipids and lipoproteins in children with kwashiorkor.British Medical Journal 5984, 630–636.CrossRefGoogle Scholar
Seakins, AL and Waterlow, JC (1972) Effect of a low protein diet in incorporation of amino acids into rat serum lipoproteins.Biochemistry Journal 129, 793–795.CrossRefGoogle ScholarPubMed
Soteriou, A and Cryer, A (1993) Purification and characterization of lipoprotein lipase from the white adipose, skeletal muscle, cardiac muscle, mammary gland and lung tissues of the rat.International Journal of Biochemistry 25, 1483–1490.CrossRefGoogle ScholarPubMed
Truswell, AS, Hansen, JD, Watson, CE and Wannenburg, P (1969) Relation of serum lipids and lipoprotein to fatty liver in kwashiorkor.American Journal of Clinical Nutrition 22, 568–576.CrossRefGoogle ScholarPubMed
Waterlow, JC (1975) Amount and rate of disappearance of liver fat in malnourished infants in Jamaica.American Journal of Clinical Nutrition 2, 1330–1336.CrossRefGoogle Scholar