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Low-protein diet prevents tissue lipoprotein lipase activity increase in growing rats

Published online by Cambridge University Press:  09 March 2007

A. Boualga ,
M. Bouchenak  and
J. Belleville
A. Boualga
Affiliation:
Laboratoire de Physiologie Animale et de la Nutrition, Facultè des Sciences, Universitè d'Oran, Es-Sènia, 31 000 Oran, Algeria
M. Bouchenak
Affiliation:
Laboratoire de Physiologie Animale et de la Nutrition, Facultè des Sciences, Universitè d'Oran, Es-Sènia, 31 000 Oran, Algeria
J. Belleville*
Affiliation:
Unitè de Nutrition Cellulaire et Mètabolique, Facultè des Sciences Mirande, Universitè de Bourgogne, BP 400, 21011 Dijon Cedex, France
*
*Corresponding author: Dr J. Belleville, fax +33 3 80 39 63 30, email [email protected]
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Abstract

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The time course of changes in tissue lipolytic activities was studied in young rats during the consumption of a low-protein diet containing 50 g protein/kg (40 g wheat gluten +10 g casein/kg) for 28 d followed by balanced refeeding with 200 g protein/kg (160 g wheat gluten +40 g casein/kg) for 28 d. Lipoprotein lipase (LPL) activities were compared with the values of a control group fed a balanced diet containing 200 g protein/kg for 56 d. At the end of protein malnutrition period, the epididymal fat tissue LPL activity represented 36 %, and that of heart and gastrocnemius was 44 %, of those of the control group. These differences were accompanied by lower serum- and VLDL-triacylglycerols (TAG), respectively 47·6 % and 31 % of the control group values, probably resulting from reduced synthesis of VLDL-apolipoproteins (29 % of control group values), concomitant with liver lipid accumulation (4·8-fold) and little lipid storage in epididymal fat tissue. At day 2 of refeeding, there was no significant difference in liver and epididymal fat tissue LPL activities between experimental and control rats. At the end of the refeeding period, LPL activity of epididymal fat and liver lipolytic activity had increased and became similar to control group values. The consumption of a low-protein diet prevented the increase in extrahepatic LPL activities as observed in the control group. The alterations in LPL activity suggest that a low-protein diet limits lipid storage in adipose tissue due to reduced serum VLDL-TAG availability.

Keywords

Lipoprotein lipaseProtein malnutritionBalanced refeedingRat
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 5 , November 2000 , pp. 663 - 671
Copyright
Copyright © The Nutrition Society 2000

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