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Lipid metabolism of liquid-associated and solid-adherent bacteria gin rumen contents of dairy cows offered lipid-supplemented diets

Published online by Cambridge University Press:  09 March 2007

D. Bauchart ,
F. Legay- Carmier ,
M. Doreau  and
B. Gaillard
D. Bauchart
Affiliation:
Laboratoire d'Etude du Métabolisme EnergétiqueINRA CRZV, Theix, 63122 Saint-Genés CharnpanelleFrance
F. Legay- Carmier
Affiliation:
Laboratoire d'Etude du Métabolisme EnergétiqueINRA CRZV, Theix, 63122 Saint-Genés CharnpanelleFrance
M. Doreau
Affiliation:
Laboratoire de la LactationINRA CRZV, Theix, 63122 Saint-Genés CharnpanelleFrance
B. Gaillard
Affiliation:
Laboratoire de Microbiologie, INRA CRZV, Theix, 63122 Saint-Genés Champanelle, France
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Abstract

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The lipid distribution and fatty acid (FA) composition of total lipids, polar lipids and free fatty acids (FFA) were determined in liquid-associated bacteria (LAB) and solid-adherent bacteria (SAB) isolated from the rumen contents of seven dairy cows fitted with rumen fistulas. Two experiments, arranged according to a 4 x 4 and 3 x 3 Latin Square design, were performed using two basal diets consisting of one part hay and one part barley-based concentrate, and five lipid-supplemented diets consisting of the basal diet plus (g/kg dry matter): 53 or 94 rapeseed oil, 98 tallow, 87 soya-bean oil or 94 palmitostearin. For all diets used, total lipids were 1.7–2.2 times higher in SAB than in LAB (P < 005); this probably resulted from a preferential incorporation of dietary FA adsorbed onto food particles. Addition of oil or fat to the diets did not modify the polar lipid content but increased the FFA content of SAB and LAB by 150%. Lipid droplets were observed in the cytoplasm in SAB and LAB using transmission electron microscopy, which suggested that part of the additional FFA was really incorporated into the intracellular FFA rather than associated with the cell envelope by physical adsorption. Linoleic acid was specifically incorporated into the FFA of SAB, which emphasized the specific role of this bacterial compartment in the protection of this acid against rumen biohydrogenation.

Keywords

Rumen bacteriaLipid metabolismLipid-supplemented diets
Type
Lipid Metebolism
Information
British Journal of Nutrition , Volume 63 , Issue 3 , May 1990 , pp. 563 - 578
Copyright
Copyright © The Nutrition Society 1990

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