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Linoleic acid and arachidonic acid metabolism in human peripheral blood leucocytes: comparison with the rat

Published online by Cambridge University Press:  09 March 2007

Stephen C. Cunnane ,
P. W. Napoleon Keeling ,
Richard P. H. Thompson  and
Michael A. Crawford
Stephen C. Cunnane
Affiliation:
Department of Nutritional Biochemistry, Nuffield Laboratories of Comparative Medicine, Institute of Zoology, London NW 1 4RY
P. W. Napoleon Keeling
Affiliation:
Gastrointestinal Laboratory, The Rayne Institute, St Thomas's Hospital, London SE1 7EH
Richard P. H. Thompson
Affiliation:
Gastrointestinal Laboratory, The Rayne Institute, St Thomas's Hospital, London SE1 7EH
Michael A. Crawford
Affiliation:
Department of Nutritional Biochemistry, Nuffield Laboratories of Comparative Medicine, Institute of Zoology, London NW 1 4RY
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Abstract

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1. Peripheral blood leucocytes from human male volunteers and from male rats were incubated in vitro in the presence of 14C-labelled linoleic acid ([14C]LA) or 3H-labelled arachidonic acid ([3H]AA). The time-course of [14C]LA and [3H]AA incorporation into human leucocyte total lipids was maximal at 80–90% of the initial dose within 20–30 min of dosing the cells.

2. Compared with mixed leucocytes, isolated polymorphonuclear leucocytes were only marginally different in the differential incorporation of [14C]LA and [3H]AA into total lipids.

3. In human leucocytes, [14C]LA was incorporated initially into triglycerides but predominantly into phosphatidylcholine thereafter. In the rat, [14C]LA remained as the free acid (63%), with lesser amounts entering the phospholipids (9%), monoglycerides-diglycerides (12%) and triglycerides (< 1%).

4. Utilization of [14C]LA by the Δ6-desaturase was only a minor route of its metabolism in both human and rat leucocytes.

5. 3H-labelled prostaglandins E2 and F2α accounted for up to 30% of the radioactivity released into the incubation medium from human leucocytes incubated with [3H]AA for 60 min.

6. Stimulation of phagocytosis in the human leucocytes with latex beads or with unopsonized zymosan did not alter the differential incorporation of [14C]LA or [3H]AA into the leucocyte lipid fractions.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 51 , Issue 2 , March 1984 , pp. 209 - 217
Copyright
Copyright © The Nutrition Society 1984

References

Bills, T. K., Smith, J. B. & Silver, M. J. (1977). Journal of Clinical Investigation 60, 1–6.CrossRefGoogle Scholar
Bonney, R. J., Wightman, P. D., Dalgren, M. E., Humes, J. L. & Davies, P. (1981). Scandinavian Journal of Rheumatology, Supplement, 40, 53–57.CrossRefGoogle Scholar
Bonney, R. J., Wightman, P. D., Davies, P., Sadowski, S. J., Kuehl, F. A. Jr & Humes, J. L. (1978). Biochemical Journal 176, 433–442.CrossRefGoogle Scholar
Borgeat, P., Hamberg, M. F. & Samuelsson, B. (1976). Journal of Biological Chemistry 251, 7816–7820.CrossRefGoogle Scholar
Cook, H. W., Clarke, J. T. R. & Spence, M. W. (1983). Prostaglandins, Leukotrienes and Medicine 10, 39–52.CrossRefGoogle Scholar
Cunnane, S. C. & Huang, Y–S. (1982). Proceedings of the Fifth International Conference on Prostaglandins, Florence, Abstract 702.Google Scholar
Cunnane, S. C. & Wahle, K. W. J. (1981). Lipids 16, 771–774.CrossRefGoogle Scholar
Elsbach, P. (1963). Biochimica Biophysica Acta 70, 157–167.CrossRefGoogle Scholar
Elsbach, P. (1964). Biochimica Biophysica Acta 84, 8–17.Google Scholar
Elsbach, P. (1972). Seminars in Haematology 9, 227–239.Google Scholar
Hansen, H. E., Knott, E. M. & Weise, H. F. (1947). American Journal of Diseases of Childhood 73, 1–18.Google Scholar
Keeling, P. W. N., Jones, R. B., Hilton, P. J. & Thompson, R. P. H. (1981). Gut 21, 561–564.CrossRefGoogle Scholar
Morley, J. (1981). In Lymphokines, Vol. 4, A Forum for Immunoregulatory Cell Products, pp. 377–394 [Pike, E., editor]. New York: Academic Press.Google Scholar
Scott, W. A., Zrike, J. M., Hamill, A. L., Kempe, J. & Cohn, Z. A. (1980). Journal of Experimental Medicine 152, 324–335.CrossRefGoogle Scholar
Stone, K. J., Willis, A. L., Hart, M., Kirtland, S. J., Kernoff, P. B. A. & McNichol, P. B. (1979). Lipids 14,174–180.CrossRefGoogle Scholar