Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-05T21:00:40.464Z Has data issue: false hasContentIssue false
  • English
  • Français

Lactococcus lactis is capable of improving the riboflavin status in deficient rats

Published online by Cambridge University Press:  08 March 2007

Jean Guy LeBlanc ,
Catherine Burgess ,
Fernando Sesma ,
Graciela Savoy de Giori  and
Douwe van Sinderen
Jean Guy LeBlanc*
Affiliation:
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, Tucumán, T4000ILC, Argentina
Catherine Burgess
Affiliation:
Department of Microbiology and Biosciences Institute, National University of Ireland Cork, Western Road, Cork, Republic of Ireland
Fernando Sesma
Affiliation:
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, Tucumán, T4000ILC, Argentina
Graciela Savoy de Giori
Affiliation:
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, Tucumán, T4000ILC, Argentina Cátedra de Microbiología Superior, Universidad Nacional de Tucumán (UNT), Tucumán, T4000ILC, Argentina
Douwe van Sinderen
Affiliation:
Department of Microbiology and Biosciences Institute, National University of Ireland Cork, Western Road, Cork, Republic of Ireland Alimentary Pharmabiotic Centre, Biosciences Institute, National University of Ireland Cork, Western Road, Cork, Republic of Ireland
*
*Corresponding author: Jean Guy LeBlanc, fax +54 0381 400 5600, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 ‘factory’ by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion–repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.

Keywords

RiboflavinLactic acid bacteriaAriboflavinosisGenetically modified micro-organisms
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 2 , August 2005 , pp. 262 - 267
Copyright
Copyright © The Nutrition Society 2005

References

Adelekan, DA & Thurnham, DI (1986) The influence of riboflavin deficiency on absorption and liver storage of iron in the growing rat. Br J Nutr 56, 171–179.CrossRefGoogle ScholarPubMed
Bailey, AL, Maisey, S, Southon, S, Wright, AJ, Finglas, PM & Fulcher, RA (1997) Relationships between micronutrient intake and biochemical indicators of nutrient adequacy in a free-living elderly UK population. Br J Nutr 77, 225–242.CrossRefGoogle Scholar
Bates, C (1993) Riboflavin.Int J Vitam Nutr Res 63, 274–277.Google ScholarPubMed
Benton, D, Haller, J & Fordy, J (1997) The vitamin status of young British adults. Int J Vitam Nutr Res 67, 34–40.Google ScholarPubMed
Boisvert, WA, Castaneda, C, Mendoza, I, Langeloh, G, Solomons, NW, Gershoff, SN & Russell, RM (1993) Prevalence of riboflavin deficiency among Guatemalan elderly people and its relationship to milk intake. Am J Clin Nutr 58, 85–90.CrossRefGoogle ScholarPubMed
Burgess, C, O'Connell-Motherway, M, Sybesma, W, Hugenholtz, J & Van Sinderen, D (2004) Riboflavin production in Lactococcus lactis: potential for in situ production of vitamin-enriched foods. Appl Environ Microbiol 70, 5769–5777.CrossRefGoogle ScholarPubMed
Butler, BF & Topham, RW (1993) Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. Biochem Mol Biol Int 30, 53–61.Google ScholarPubMed
Capo-Chichi, CD, Gueant, JL, Feillet, F, Namour, F & Vidailhet, M (2000) Analysis of riboflavin and riboflavin cofactor levels in plasma by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 739, 219–224.CrossRefGoogle ScholarPubMed
Glatzle, D, Körner, WF, Christeller, S & Wiss, O (1970) Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH2-dependent glutathione reductase from human erythrocytes by FAD in vitro. Investigations on the vitamin B2 status in healthy people and geriatric patients. Int Z Vitaminforsch 40, 166–183.Google Scholar
Glatzle, D, Weber, F & Wiss, O (1968) Enzymatic test for the detection of a riboflavin deficiency. NADPH-dependent glutathione reductase of red blood cells and its activation by FAD in vitro. Experientia 24 1122.CrossRefGoogle ScholarPubMed
Greene, HL, Specker, BL, Smith, R, Murrell, J & Swift, L (1990) Plasma riboflavin concentrations in infants fed human milk versus formula: comparison with values in rats made riboflavin deficient and human cord blood J Pediatr 117, 916–920.CrossRefGoogle ScholarPubMed
Hustad, S, McKinley, MC, McNulty, H, Schneede, J, Strain, JJ, Scott, JM & Ueland, PM (2002) Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clin Chem 48, 1571–1577.CrossRefGoogle ScholarPubMed
Hustad, S, Ueland, PM, Vollset, SE, Zhang, Y, Bjørke-Monsen, AL & Schneede, J (2000) Riboflavin as a determinant of plasma total homocysteine: effect modification by the methylenetetrahydrofolate reductase C677T polymorphism. Clin Chem 46, 1065–1071.CrossRefGoogle ScholarPubMed
Lakshmi, R, Lakshmi, AV, Divan, PV & Bamji, MS (1991) Effect of riboflavin or pyridoxine deficiency on inflammatory response. Indian J Biochem Biophys 28, 481–484.Google ScholarPubMed
LeBlanc, JG, Burgess, C, Sesma, F, Savoy de Giori, G & Van Sinderen, D (2005) Ingestion of milk fermented by genetically modified Lactococcus lactis improves the riboflavin status of deficient rats. J Dairy Sci (In the Press).CrossRefGoogle ScholarPubMed
Madigan, SM, Tracey, F, McNulty, H, Eaton-Evans, J, Coulter, J, McCartney, H & Strain, JJ (1998) Riboflavin and vitamin B-6 intakes and status and biochemical response to riboflavin supplementation in free-living elderly people. Am J Clin Nutr 68, 389–395.CrossRefGoogle ScholarPubMed
Institute for Laboratory Animal Research (1995) Nutrient Requirements of Laboratory Animals 4th revised ed. Washington, DC: National Academy Press.Google Scholar
Ogunleye, AJ & Odutuga, AA (1989) The effect of riboflavin deficiency on cerebrum and cerebellum of developing rat brain. J Nutr Sci Vitaminol (Tokyo) 35, 193–197.CrossRefGoogle ScholarPubMed
Pangrekar, J, Krishnaswamy, K & Jagadeesan, V (1993) Effects of riboflavin deficiency and riboflavin administration on carcinogen-DNA binding. Food Chem Toxicol 31, 745–750.CrossRefGoogle ScholarPubMed
Powers, HJ (1987) A study of maternofetal iron transfer in the riboflavin-deficient rat. J Nutr 117, 852–856.CrossRefGoogle ScholarPubMed
Powers, HJ, Weaver, LT, Austin, S & Beresford, JK (1993) A proposed intestinal mechanism for the effect of riboflavin deficiency on iron loss in the rat. Br J Nutr 69, 553–561.CrossRefGoogle ScholarPubMed
Powers, HJ, Weaver, LT, Austin, S, Wright, AJ & Fairweather-Tait, SJ (1991) Riboflavin deficiency in the rat: effects on iron utilization and loss. Br J Nutr 65, 487–496.CrossRefGoogle ScholarPubMed
Powers, HJ, Wright, AJ & Fairweather-Tait, SJ (1988) The effect of riboflavin deficiency in rats on the absorption and distribution of iron. Br J Nutr 59, 381–387.CrossRefGoogle Scholar
Webster, RP, Gawde, MD & Bhattacharya, RK (1996) Modulation of carcinogen-induced DNA damage and repair enzyme activity by dietary riboflavin. Cancer Lett 98, 129–135.CrossRefGoogle ScholarPubMed
Williams, EA, Powers, HJ & Rumsey, RD (1995) Morphological changes in the rat small intestine in response to riboflavin depletion. Br J Nutr 73, 141–146.CrossRefGoogle ScholarPubMed
Williams, EA, Rumsey, RD & Powers, HJ (1996) Cytokinetic and structural responses of the rat small intestine to riboflavin depletion. Br J Nutr 75, 315–324.CrossRefGoogle ScholarPubMed
Yates, CA, Evans, GS, Pearson, T & Powers, HJ (2003) Absence of luminal riboflavin disturbs early postnatal development of the gastrointestinal tract. Dig Dis Sci 48, 1159–1164.CrossRefGoogle ScholarPubMed
Yates, CA, Evans, GS & Powers, HJ (2001) Riboflavin deficiency: early effects on post-weaning development of the duodenum in rats. Br J Nutr 86, 593–599.CrossRefGoogle ScholarPubMed