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Intestinal degradation in pigs of rye dietary fibre with different structural characteristics

Published online by Cambridge University Press:  09 March 2007

L. V. Glitsø ,
G. Brunsgaard ,
S. Højsgaard ,
B. Sandström  and
K. E. Bach Knudsen
L. V. Glitsø*
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, PO Box 50, Research Centre Foulum, 8830 Tjele, Denmark Research Department of Human Nutrition, The Royal Veterinary and Agricultural University, Rolighedsvej 30, 1958 Frederiksberg C, Copenhagen, Denmark
G. Brunsgaard
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, PO Box 50, Research Centre Foulum, 8830 Tjele, Denmark
S. Højsgaard
Affiliation:
Department of Agricultural Systems, PO Box 23, Research Centre Foulum, 8830 Tjele, Denmark
B. Sandström
Affiliation:
Research Department of Human Nutrition, The Royal Veterinary and Agricultural University, Rolighedsvej 30, 1958 Frederiksberg C, Copenhagen, Denmark
K. E. Bach Knudsen
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, PO Box 50, Research Centre Foulum, 8830 Tjele, Denmark
*
*Corresponding author:Dr Vibe Glitsø, fax +45 8999 1378, email [email protected]
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Abstract

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In order to investigate the effects of dietary fibre (DF) characteristics on carbohydrate degradation and the metabolism in the large intestine, pigs were fed on four rye-bread diets (based on whole rye, pericarp/testa, aleurone or endosperm) with differences in characteristics and amount of DF. The degradability of DF in the large intestine varied greatly between diets. The pericarp/testa DF was hardly degraded in the large intestine, whereas endosperm DF was extensively and rapidly degraded in the caecum. Caecal degradation of aleurone DF was also limited, leaving more material to be degraded in the colon. The undegradable pericarp/testa DF was characterized by high contents of lignin, cellulose, ferulic acids and highly substituted arabinoxylans (the major DF component in rye). Ingestion of this diet resulted in increased faecal bulk and reduced transit time, but with low colonic pH and the lowest concentrations of short-chain fatty acids (SCFA). The aleurone diet, on the other hand, led to a fermentation pattern which may be considered more optimal, with lower colonic pH and higher concentrations of SCFA, in particular butyric acid. Despite the large differences in carbohydrate fermentation only minor significant effects on the presence of protein degradation products and on histological measurements (height and diameter of colonic crypts and thickness of the muscularis externa) were observed.

Keywords

RyeDietary fibreFermentationPigs
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 5 , November 1998 , pp. 457 - 468
Copyright
Copyright © The Nutrition Society 1998

References

Andrieux, C, Gadelle, D, Leprince, C & Sacquet, E (1989) Effects of some poorly digestible carbohydrates on bile acid bacterial transformations in the rat. British Journal of Nutrition 62, 103–119.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (1990) Official Methods of Analysis, 15th ed., p. 777. Washington, DC: AOAC.Google Scholar
Auffret, A, Barry, JL & Thibault, J-F (1993) Effect of chemical treatments of sugar beet fibre on their physico-chemical properties and on their in-vitro fermentation. Journal of the Science of Food and Agriculture 61, 195–203.CrossRefGoogle Scholar
Bach Knudsen KE (1997) Carbohydrate and lignin contents of plant materials used in animal feeding. Animal Feed Science and Technology 67, 319–338.CrossRefGoogle Scholar
Bach Knudsen, KE, Jensen, BB & Hansen, I (1993) Oat bran but not a beta-glucan-enriched oat fraction enhances butyrate production in the large intestine of pigs. Journal of Nutrition 123, 1235–1247.CrossRefGoogle Scholar
Bach Knudsen, KE, Steenfeldt, S, B¢rsting, CF & Eggum, BO (1995) The nutritive value of decorticated mill fractions of wheat. 1. Chemical composition of raw and enzyme treated fractions and balance experiments with rats. Animal Feed Science and Technology 52, 205–225.CrossRefGoogle Scholar
Bingham, SA (1990) Mechanisms and experimental and epidemio-logical evidence relating dietary fibre (non-starch polysaccharides) and starch to protection against large bowel cancer. Proceedings of the Nutrition Society 49, 153–171.CrossRefGoogle Scholar
Bourquin, LD, Titgemeyer, EC, Garleb, KA & Fahey, GC (1992) Short-chain fatty acid production and fiber degradation by human colonic bacteria: effects of substrate and cell wall fractionation. Journal of Nutrition 122, 1508–1520.CrossRefGoogle ScholarPubMed
Brøbech Mortensen, P & Rye Clausen, M (1996) Short-chain fatty acids in the human colon: Relation to gastrointestinal health and disorders. Scandinavian Journal of Gastroenterology 31, Suppl. 1, 132–148.CrossRefGoogle Scholar
Bruce, WR (1987) Recent hypothesis for the origin of colon cancer. Cancer Research 47, 4237–4242.Google Scholar
Brunsgaard, G (1997) Morphological characteristics, epithelial cell proliferation, and crypt fission in caecum and colon of growing pigs. Digestive Diseases and Sciences 41, 2384–2393.CrossRefGoogle Scholar
Carpita, NC & Gibeaut, DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the wall during growth. Plant Journal 3, 1–30.CrossRefGoogle ScholarPubMed
Cornu, A, Besle, JM, Mosoni, P & Grenet, E (1994) Lignin–carbohydrate complexes in forages: structures and consequences in the ruminal degradation of cell wall carbohydrates. Reproduction, Nutrition and Development 34, 385–398.CrossRefGoogle ScholarPubMed
Cummings, JH (1993) Dietary Fibre Intakes in Europe. Cost 92, pp. 11–21. Brussels: Commission of the European Communities.Google Scholar
Cummings, JH, Gibson, GR & Macfarlane, GT (1989) Quantitative estimates of fermentation in the hind gut of man. Acta Veterinaria Scandinavica 86, Suppl., 76–82.Google ScholarPubMed
Cummings, JH, Hill, MJ, Jivraj, T, Houston, H, Branch, WJ & Jenkins, DJA (1979) The effect of meat protein and dietary fibre on colonic function and metabolism. II. Bacterial metabolites in feces and urine. American Journal of Clinical Nutrition 32, 2094–2101.CrossRefGoogle Scholar
Cummings, JH & Macfarlane, GT (1991) The control and consequences of bacterial fermentation in the human colon. Journal of Applied Bacteriology 70, 443–459.CrossRefGoogle ScholarPubMed
Eastwood, MA (1992) The physiological effect of dietary fibre: an update. Annual Review of Nutrition 12, 19–35.CrossRefGoogle ScholarPubMed
Fincher, GB & Stone, BA (1986) Cell walls and their components in cereal grain technology. In Cereal Science and Technology, vol. VIII, pp. 207–295 [Pomeranz,, Y editor]. St. Paul, MN: American Association of Cereal Chemists.Google Scholar
Frankel, WL, Zhang, W, Singh, A, Klurfeld, DM, Dan, S, Sakata, T, Modlin, I & Rombeau, JL (1994) Mediation of the trophic effects of short-chain fatty acids on the rat jejunum and colon. Gastroenterology 106, 375–380.CrossRefGoogle ScholarPubMed
Glitsø, LV (1997) Rye dietary fibre – structure and metabolism in the large intestine of pigs. PhD Thesis, Royal Veterinary and Agricultural University, Denmark.Google Scholar
Graham, H, Hesselman, K, Jonsson, E & Åman, P (1986) Influence of beta-glucanase supplementation on digestion of a barley-based diet in the pig gastro-intestinal tract. Nutrition Reports International 34, 1089–1096.Google Scholar
Hromádková, Z & Ebringerová, A (1992) Relation between extractability and structural heterogeneity of rye bran D-xylan. In Xylans and Xylanases, pp. 395–399 [Visser,, J, Beldman,, G, Kusters-van Someren, MA and Voragen, AGJ, editors]. Amsterdam: Elsevier Science Publishers B.V.Google Scholar
Iiyama, K, Lam, TB-T & Stone, BA (1990) Phenolic acid bridges between polysaccharides and lignin in wheat internodes. Phyto-chemistry 29, 733–737.Google Scholar
Iiyama, K, Lam, TB-T & Stone, BA (1994) Covalent cross-links in the cell wall. Plant Physiology 104, 315–320.CrossRefGoogle ScholarPubMed
Jacobs, LR (1986) Modification of experimental colon carcino-genesis by dietary fibre. Advances in Experimental Medicine and Biology 206, 105–118.Google Scholar
Jensen, MT, Cox, RP & Jensen, BB (1995 a) Microbial production of skatole in the hind gut of pigs given different diets and its relation to skatole deposition in the back fat. Animal Science 61, 293–304.CrossRefGoogle Scholar
Jensen, MT, Cox, RP & Jensen, BB (1995 b) 3-Methylindole (skatole) and indole production by mixed populations of pig fecal bacteria. Applied Environmental Microbiology 61, 3180–3184.CrossRefGoogle ScholarPubMed
Johansen, HN, Bach Knudsen, KE, Sandström, B & Skj¢th, F (1996) Effects of varying content of soluble dietary fibre from wheat flour and oat milling fractions in gastric emptying in pigs. British Journal of Nutrition 75, 339–351.CrossRefGoogle ScholarPubMed
Larsson, K & Bengtsson, S (1983) Bestämning av Lätt Tillgängliga Kolhydrater i Växtmaterial (Determination of Readily Available Carbohydrates in Plant Material). National Laboratory of Agricultural Chemistry, Method Report no. 22. Uppsala: National Laboratory of Agricultural Chemistry.Google Scholar
Leisola, MSA & Garcia, S (1989) The mechanisms of lignin degradation. In Enzyme Systems for Lignocellulose Degradation, pp. 89–99 [Coughlan,, MP editor]. Amsterdam: Elsevier Applied Science.Google Scholar
Lund, EK, Salf, KL & Johnson, IT (1993) Baked rye products modify cholesterol metabolism and crypt cell proliferation rates in rats. Journal of Nutrition 123, 1834–1843.CrossRefGoogle ScholarPubMed
Lundin, E, Zhang, J-X, Huang, C-B, Reuterving, C-O, Hallmans, G, Nygren, C & Stenling, R (1993) Oat bran, rye bran, and soybean hull increase goblet cell volume density in the small intestine of the golden hamster. Scandinavian Journal of Gastroenterology 28, 15–22.CrossRefGoogle ScholarPubMed
McBurney, MI, Van Soest, PJ & Jeraci, JL (1987) Colonic carcino-genesis: the microbial feast or famine mechanism. Nutrition and Cancer 10, 23–28.CrossRefGoogle ScholarPubMed
McCleary, BV & Glennie-Holmes, M (1985) Enzymatic quantification of (1–3)(1–4)-β-D-glucan in barley and malt. Journal of the Institute of Brewing 91, 285–295.CrossRefGoogle Scholar
McDougall, GJ, Morrison, IM, Stewart, D & Hillman, JR (1996) Plant cell walls as dietary fibre: range, structure, processing and function. Journal of the Science of Food and Agriculture 70, 133–150.3.0.CO;2-4>CrossRefGoogle Scholar
McIntyre, A, Gibson, PR & Young, GP (1993) Butyrate production from dietary fibre and protection against large bowel cancer in a rat model. Gut 34, 386–391.CrossRefGoogle ScholarPubMed
Sakata, T (1987) Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects of fermentable fibre, gut microbes and luminal trophic factors. British Journal of Nutrition 58, 95–103.CrossRefGoogle ScholarPubMed
Salvador, V, Cherbut, C, Barry, JL, Bertrand, D, Bonnet, C & Delort-Laval, J (1993) Sugar composition of dietary fibre and short-chain fatty acid production during in vitro fermentation by human bacteria. British Journal of Nutrition 70, 189–197.CrossRefGoogle ScholarPubMed
Schooneveld-Bergmans, MEF (1997) Wheat bran glucuronoarabinoxylans. PhD Thesis, Wageningen Agricultural University, The Netherlands.Google Scholar
Schürch, AF, Lloyd, LE & Crampton, EW (1950) The use of chromic oxide as an index for determining the digestibility of a diet. Journal of Nutrition 50, 628–636.Google Scholar
Searle, SR, Casella, G & McCulloch, CE (1992) Variance Components. New York, NY: Wiley.CrossRefGoogle Scholar
Stephen, AM & Cummings, JH (1980) Mechanism of action of dietary fibre in the human colon. Nature 284, 283–284.CrossRefGoogle ScholarPubMed
Stoldt, W (1952) Vorschlag zur Vereinheitlichung der Fettbestim-mung in Lebensmitteln (Suggestion to standardise the determination of fat in foodstuffs). Fette, Seifen unt Anstrichmittelen 54, 206–207.CrossRefGoogle Scholar
Thornton, JR (1981) High colonic pH promotes colorectal cancer. Lancet 1, 1081–1082.CrossRefGoogle ScholarPubMed
Van Dokkum, W, Pikaar, NA & Thiessen, JTNM (1983) Physiological effects of fibre-rich types of bread. 2. Dietary fibre from bread: digestibility by the intestinal microflora and water-holding capacity in the colon of human subjects. British Journal of Nutrition 50, 61–74.Google ScholarPubMed
Van Munster, IP & Nagengast, FM (1993) The role of carbohydrate fermentation in colon cancer prevention. Scandinavian Journal of Gastroenterology 200, 80–86.CrossRefGoogle ScholarPubMed
Verbruggen, M (1996) Glucuronoarabinoxylans from sorghum grain. PhD Thesis, Wageningen Agricultural University, The Netherlands.Google Scholar
Vinkx, CJA & Delcour, JA (1996) Rye (Secale cereale) arabinoxylans: a critical review. Journal of Cereal Science 24, 1–14.CrossRefGoogle Scholar
Wisker, E, Bach, Knudsen KE, Daniel, M, Feldheim, W & Eggum, BO (1996) Digestibilities of energy, protein, fat and nonstarch polysaccharides in a low fibre diet and diets containing coarse or fine whole meal rye are comparable in rats and humans. Journal of Nutrition 126, 481–488.CrossRefGoogle ScholarPubMed