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Interactive effects of increased intake of saturated fat and cholesterol on atherosclerosis in the Japanese quail (Coturnix japonica)

Published online by Cambridge University Press:  22 August 2007

Yvonne V. Yuan ,
David D. Kitts  and
David V. Godin
Yvonne V. Yuan*
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of British Columbia, 6650 NW Marine Drive, Vancouver, British Columbia, Canada V6T 1Z4
David D. Kitts
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of British Columbia, 6650 NW Marine Drive, Vancouver, British Columbia, Canada V6T 1Z4
David V. Godin
Affiliation:
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, 2176 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3
*
†Corresponding author: Dr David Kitts, fax +1 604 822 3959, email [email protected]
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Abstract

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Increasing the energy value of diets with dietary fat, particularly fats rich in saturated fatty acids, can result in the elevation of plasma total and lipoprotein cholesterol. In the present study, experimental diets were designed to examine the effects of increasing the energy content of diets with a saturated fat source and cholesterol in a non-purified diet on hyperlipoproteinaemia and aortic plaque composition in the atherosclerosis-susceptible Japanese quail (Coturnix japonica) model of human atherosclerosis. Commercial poultry diets containing two levels (i.e. 60 or 120 g/kg) of beef tallow as the primary source of saturated fat were balanced for endogenous cholesterol or supplemented with cholesterol (i.e. 0·5 or 5·0 g/kg) and fed to quail for 9 weeks to examine the effects on whole plasma, lipoprotein and aortic plaque lipid composition in relation to aortic plaque formation. Hypercholesterolaemia (P<0·001) was confirmed in birds fed on high-cholesterol (HC) diets only. An interaction (P=0·05) between dietary cholesterol and fat intake level was observed for plasma triacylglycerols (TG) and was specific to changes observed in VLDL composition. Diet-induced changes in lipoprotein total cholesterol, TG and phospholipid composition were greatest in the portomicron and VLDL fractions in birds fed on atherogenic diets. Hyperlipoproteinaemia induced by the 60 g/kg added beef tallow–HC diet resulted in significant (P<0·001) aortic plaque deposition, which was further enhanced in birds fed on the 120 g/kg beef tallow–HC diet. Quail fed on 120 g/kg beef tallow-HC diets exhibited the most severe aortic plaque formation, with marked increases in aortic tissue cholesterol content and quantifiable amounts of several cholesterol oxides (5,6α-epoxy-5α-cholesterol, 7β-hydroxycholesterol, cholestanetriol, 7-ketocholesterol and 25-hydroxycholesterol). In summary, hyperlipoproteinaemia associated with HC diets with a greater proportion of energy from saturated fat produced a combined effect in altering plasma and lipoprotein lipid composition as well as aortic tissue cholesterol and cholesterol oxide content in the Japanese quail.

Keywords

AtherosclerosisJapanese quailLipoproteinsCholesterol
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 1 , July 1998 , pp. 89 - 100
Copyright
Copyright © The Nutrition Society 1998

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