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Insulin response and changes in composition of non-esterified fatty acids in blood plasma of middle-aged men following isoenergetic fatty and carbohydrate breakfasts

Published online by Cambridge University Press:  09 March 2007

D. L. Frape ,
N. R. Williams ,
K. L. H. Carpenter ,
M. A. Freeman ,
C. R. Palmer  and
R. J. Fletcher
D. L. Frape*
Affiliation:
NS Research, The Priory, Churchyard, Mildenhall, Suffolk IP28 7EE, UK Pathology Department, Papworth Hospital, Cambridge CB3 8RE, UK
N. R. Williams
Affiliation:
Pathology Department, Papworth Hospital, Cambridge CB3 8RE, UK
K. L. H. Carpenter
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
M. A. Freeman
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
C. R. Palmer
Affiliation:
Centre for Applied Medical Statistics, University of Cambridge, Robinson Way, Cambridge CB2 2SR, UK
R. J. Fletcher
Affiliation:
The Kellogg Company of Great Britain Ltd, The Kellogg Building, Talbot Road, Manchester M16 0PU, UK
*
*Corresponding author:Dr David Frape, fax +44 1638 712192, email [email protected]
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Abstract

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It was previously shown that a high plasma concentration of non-esterified fatty acids (NEFA) persisted after a fatty breakfast, but not after an isoenergetic carbohydrate breakfast, adversely affecting glucose tolerance. The higher concentration after the fatty breakfast may in part have been a result of different mobilization rates of fatty acids. This factor can be investigated as NEFA mobilized from tissues are monounsaturated to a greater extent than those deposited from a typical meal. Twenty-four middle-aged healthy Caucasian men were given oral glucose tolerance tests (OGTT), and for 28 d isoenergetic breakfasts of similar fat composition but of low (L) or moderate (M) fat content. The composition of NEFA in fasting and postprandial plasma was determined on days 1 and 29. No significant treatment differences in fasting NEFA composition occurred on day 29. During the OGTT and 0–1 h following breakfast there was an increase in plasma long-chain saturated NEFA but a decrease in monounsaturated NEFA (μg/100 μg total NEFA; P<0·001). Between 1 and 3 h following breakfast treatment differences occurred for total saturated and total monounsaturated fatty acids (μg/100 μg total NEFA; P<0·05), expressed as an increase in 18 : 1 and decreases in 16 : 0 and 17 : 0 in treatment M relative to treatment L (P<0·05). Serum insulin attained 35 and 65 mU/l in treatments M and L respectively during this period. Negative correlations were found between 16 : 0 in fasting plasma and both waist:hip circumference (P=0·0009) and insulin response curve area during OGTT (within treatment M, P=0·0001). It is concluded that a normal postprandial insulin response is associated with a rapid change in plasma saturated:monounsaturated NEFA. It is proposed that this change is the result of a variable suppression of fat mobilization, which may partly account for a large difference in postprandial total plasma NEFA between fatty and carbohydrate meals.

Keywords

Non-esterified fatty acidsInsulinDietary fatDietary carbohydrate
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 5 , November 2000 , pp. 737 - 745
Copyright
Copyright © The Nutrition Society 2000

References

Altman, DG (1991) Practical Statistics for Medical Research. London: Chapman and Hall.Google Scholar
Arner, P (1995) Differences in lipolysis between human subcutaneous and omental adipose tissues. Annals of Medicine 27, 435–438.CrossRefGoogle ScholarPubMed
Arner, P, Einarsson, KES and Livingstone, J (1986) Studies of the human liver insulin receptor in non-insulin-dependent diabetes mellitus. Journal of Clinical Investigation 77, 1717–1718.CrossRefGoogle Scholar
Bligh, EG and Dyer, WJA (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.CrossRefGoogle ScholarPubMed
Brook, CGD (1971) Composition of human adipose tissue from deep and subcutaneous sites. British Journal of Nutrition 25, 377–380.CrossRefGoogle ScholarPubMed
Byrne, CD, Maison, P, Halsall, D, Martensz, N, Hales, CN and Wareham, NJ (1998) Factors predicting capacity to suppress plasma non esterified fatty acid levels differ between men and women. Atherosclerosis 141, 186.Google Scholar
Connor, WE, Lin, DS and Colvis, C (1996) Differential mobilization of fatty acids from adipose tissue. Journal of Lipid Research 37, 290–298.CrossRefGoogle Scholar
Coon, PJ, Rogus, EM and Goldberg, AP (1992) Time course of plasma free fatty acid concentration in response to insulin: effect of obesity and physical fitness. Metabolism 41, 711–716.CrossRefGoogle ScholarPubMed
Ferrannini, E, Camastra, S, Coppack, SW, Fliser, D, Golay, A and Mitrakou, A (1997) Insulin action and non-esterified fatty acids. Proceedings of the Nutrition Society 56, 753–761.CrossRefGoogle ScholarPubMed
Fielding, BA and Frayn, KN (1998) Lipoprotein lipase and the disposition of dietary fatty acids. British Journal of Nutrition 80, 495–502.CrossRefGoogle ScholarPubMed
Frape, DL, Williams, NR, Rajput-Williams, J, Maitland, BW, Scriven, AJ, Palmer, CR and Fletcher, RJ (1998) Effect of breakfast fat content on glucose tolerance and risk factors of atherosclerosis and thrombosis. British Journal of Nutrition 80, 323–331.CrossRefGoogle ScholarPubMed
Frape, DL, Williams, NR, Scriven, AJ, Palmer, C, O'Sullivan, K and Fletcher, RJ (1997) The effects of high- and low-fat meals on the diurnal response of plasma lipid metabolite concentrations in healthy middle-aged volunteers. British Journal of Nutrition 77, 375–390.CrossRefGoogle Scholar
Frayn, KN (1993) Insulin resistance and lipid metabolism. Current Opinion in Lipidology 4, 197–204.CrossRefGoogle Scholar
Frayn, KN, Summers, LKM and Fielding, BA (1997) Regulation of plasma non-esterified fatty acid concentration in the postprandial state. Proceedings of the Nutrition Society 56, 713–721.CrossRefGoogle ScholarPubMed
Friderickson, DS and Gordon, RS (1958) Transport of fatty acids. Physiological Reviews 38, 602–604.Google Scholar
Friderickson, DS and Gordon, RS (1958) The metabolism of albumin-bound C14-labeled unesterified fatty acids in normal human subjects. Journal of Clinical Investigation 37, 1504–1514.CrossRefGoogle Scholar
Griffiths, AJ, Humphreys, SM, Clark, ML, Fielding, BA and Frayn, KN (1994) Immediate metabolic availability of dietary fat in combination with carbohydrate. American Journal of Clinical Nutrition 59, 53–59.CrossRefGoogle ScholarPubMed
Han, TS, Feskens, EJM, Lean, MEJ and Seidell, JC (1999) Associations of body composition with Type 2 diabetes mellitus. Diabetic Medicine 15, 129–135.3.0.CO;2-2>CrossRefGoogle Scholar
Heffernan, AGA (1963) The fatty acid composition of human adipose tissue. Clinical Science 25, 423–429.Google ScholarPubMed
Jensen, MD, Haymond, MW, Rizza, RA, Cryer, PE and Miles, JM (1989) Influence of body fat distribution on free fatty acid metabolism in obesity. Journal of Clinical Investigation 83, 1168–1173.CrossRefGoogle ScholarPubMed
Jones, AE, Stolinski, M, Smith, RD, Murphy, JL and Wootton, SA (1999) Effect of fatty acid chain length and saturation on gastrointestinal handling and metabolic disposal of dietary fatty acids in women. British Journal of Nutrition 81, 37–43.CrossRefGoogle ScholarPubMed
Kokatnur, MG, Oalmann, MC, Johnson, WD, Malcom, GT and Strong, JP (1979) Fatty acid composition of human adipose tissue from two anatomical sites in a biracial community. American Journal of Clinical Nutrition 32, 2198–2205.CrossRefGoogle Scholar
Malcom, GT, Bhattacharyya, AK, Velez-Duran, M, Guzman, MA, Oalmann, MC and Strong, JP (1989) Fatty acid composition of adipose tissue in humans: differences between subcutaneous sites. American Journal of Clinical Nutrition 50, 288–291.CrossRefGoogle ScholarPubMed
Medina, I, Auborg, S, Gallardo, JM and Perez-Martin, R (1992) Comparison of six methylation methods for analysis of the fatty acid composition of albacore lipid. International Journal of Food Science and Technology 27, 597–601.CrossRefGoogle Scholar
Pittet, PG, Halliday, D and Bateman, PE (1979) Site differences in the fatty acid composition of subcutaneous adipose tissue of obese women. British Journal of Nutrition 42, 57–61.CrossRefGoogle ScholarPubMed
Raclot, T and Groscolas, R (1993) Differential mobilization of white adipose tissue fatty acids according to chain length, unsaturation and positional isomerism. Journal of Lipid Research 34, 1515–1526.CrossRefGoogle ScholarPubMed
Smedman, AEM, Gustafsson, I-B, Berglund, LGT and Vessby, BOH (1999) Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. American Journal of Clinical Nutrition 69, 22–29.CrossRefGoogle ScholarPubMed
Svedberg, J, Björntorp P, Smith, U and Lönnroth, P (1990) Free-fatty acid inhibition of insulin binding, degradation, and action in isolated rat hepatocytes. Diabetes 39, 570–574.CrossRefGoogle ScholarPubMed
Wolfe, RR (1998) Metabolic interactions between glucose and fatty acids in humans. American Journal of Clinical Nutrition 67 (Suppl. 3), 519S–526S.CrossRefGoogle ScholarPubMed
Wolfe, RR and Peters, EJ (1987) Lipolytic response to glucose infusion in human subjects. American Journal of Physiology 252, E218–E223.Google ScholarPubMed
Wolk, A, Vessby, B, Ljung, H and Barrefors, P (1998) Evaluation of a biological marker of dairy fat intake. American Journal of Clinical Nutrition 68, 291–295.CrossRefGoogle ScholarPubMed