Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-22T23:40:42.908Z Has data issue: false hasContentIssue false

Influences of dietary methionine and cysteine on metabolic responses to immunological stress by Escherichia coli lipopolysaccharide injection, and mitogenic response in broiler chickens

Published online by Cambridge University Press:  09 March 2007

K. Takahashi
Affiliation:
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, 981 Japan
N. Ohta
Affiliation:
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, 981 Japan
Y. Akiba
Affiliation:
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, 981 Japan
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present experiments were conducted to investigate influences of dietary methionine and cysteine on metabolic responses to immunological stress induced by Escherichia coli lipopolysaccharide (LPS) injection, and concanavalin A (Con A)-induced mononuclear cell (MNC) proliferation in male broiler chickens. In Expt 1, chicks (12 d of age) were fed on a S amino acid (SAA)-deficient diet (5.6 g SAA/kg diet) or on three kinds of SAA-sufficient diet (9.3 g SAA/kg diet; low-, medium- and high-cysteine diets) which contained 2.8, 4.65 and 6.5 g cysteine/kg diet, respectively. Plasma a-1 acid glycoprotein (AGP) concentration and interleukin (IL)-1-like activity in chicks fed on the SAA-deficient diet were lower following a single injection of LPS than those in chicks fed on the SAA-sufficient diets. At 16 h after LPS injection, plasma Fe and Zn concentrations and body weight were reduced, but AGP concentration and IL-1-like activity in plasma were significantly increased. These changes in body weight, plasma Zn and Fe concentrations following injection of LPS were not affected by dietary methionine:cysteine ratios. Plasma AGP concentration and IL-1-like activity in chicks fed on the high-cysteine diet were, however, greater than those in chicks fed on the other diets following a single injection of LPS. In Expt 2, chicks (7 d of age) were fed on the SAA-sufficient diets as in Expt 1 for 10 d. MNC proliferation in spleen induced by Con A in chicks fed on the high-cysteine diet was greater than that in chicks fed on the low- or medium-cysteine diet. The results suggest that dietary cysteine has an impact on the immune and inflammatory responses.

Type
Animal Nutrition
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Boebel, K. P. & Baker, D. H. (1983) Blood and liver concentrations of glutathione and plasma concentrations of sulfur-containing amino acids in chicks fed deficient, adequate, or excess levels of dietary cysteine. Proceedings of the Society for Experimental Biology and Medicine 172, 498501.CrossRefGoogle ScholarPubMed
Droge, W., Eck, H. P., Gmunder, H. & Mihm, S. (1991) Modulation of lymphocyte functions and immune responses by cysteine and cysteine derivatives. American Journal of Medicine 30, 104S114S.Google Scholar
Enkvetechakul, B. & Bottje, W. G. (1995) Influence of diethylmaleate and cysteine on tissue glutathione and growth in broiler chickens. Poultry Science 74, 864873.CrossRefGoogle Scholar
Franklin, R. A., Li, Y. I., Arkins, S. & Kelley, K. W. (1990) Glutathione augments in-vitro proliferative responses of lymphocytes to concanavalin A to a greater degree in old than in young rats. Journal of Nutrition 120, 17101717.CrossRefGoogle ScholarPubMed
Graber, G. & Baker, D. H. (1971) Sulfur amino acid nutrition of the growing chick: quantitative aspects concentrating the efficiency of dietary methionine. Journal of Animal Science 33, 10051011.CrossRefGoogle Scholar
Grimble, R. F. (1992) Dietary manipulation of the inflammatory response. Proceedings of the Nutrition Society 51, 285294.CrossRefGoogle ScholarPubMed
Grimble, R. F., Jackson, A. A., Persaud, C., Wride, M. J., Delers, F. & Engler, R. (1992) Cysteine and glycine supplementation modulate the metabolic response to tumor necrosis factor alpha in rats fed a low protein diet. Journal of Nutrition 122, 20662073.CrossRefGoogle ScholarPubMed
Hunter, E. A. L. & Grimble, R. F. (1994) Cysteine and methionine supplementation modulate the effect of tumor necrosis factor alpha on protein synthesis, glutathione and zinc concentration of liver and lung in rats fed a low protein diet. Journal of Nutrition 124, 23192338.CrossRefGoogle ScholarPubMed
Klasing, K. C. & Barnes, D. M. (1988) Decreased amino acid requirements of growing chicks due to immunological stress. Journal of Nutrition 118, 11581164.CrossRefGoogle Scholar
National Research Council (1984) Nutrient Requirements of Poultry, 8th revised ed., pp. 3547. Washington, DC: National Academic Press.Google Scholar
Nauss, K. M., Connor, A. M., Kacancaugh, A. & Newbern, P. N. (1982) Alteration in immune function in rats caused by dietary lipotrope deficiency: effect of age. Journal of Nutrition 112, 23332341.CrossRefGoogle ScholarPubMed
Ohta, Y. & Ishibashi, I. (1994) Dietary levels and ratio of methionine and cystine for maximum performance of broilers. Japanese Poultry Science 31, 369380.CrossRefGoogle Scholar
Takahashi, K. & Akiba, Y. (1996) Effect of dietary corticosterone on mononuclear cells proliferation to mitogen in spleen and thymus of broiler chicks. Animal Science and Technology (Japan) 67, 160164.Google Scholar
Takahashi, K., Kaji, S., Akiba, Y. & Tamura, K. (1994) Plasma alpha 1 acid glycoprotein concentration in broilers: influence of age, sex and Escherichia coli lipopolysaccharide. British Poultry Science 35, 427432.CrossRefGoogle ScholarPubMed
Takahashi, K., Yodogawa, S., Akiba, Y. & Tamura, K. (1995) Effect of dietary protein concentration on responses to Escherichia coli endotoxin in broiler chickens. British Journal of Nutrition 74, 173182.CrossRefGoogle ScholarPubMed
Tsiagbe, V. K., Cook, M. E., Harper, A. E. & Sunde, M. L. (1987 a) Efficiency of cysteine in replacing methionine in the immune responses of broiler chicks. Poultry Science 66, 11381146.CrossRefGoogle Scholar
Tsiagbe, V. K., Cook, M. E., Harper, A. E. & Sunde, M. L. (1987 b) Enhanced immune responses in broiler chicks fed methionine supplemented diets. Poultry Science 58, 11471154.CrossRefGoogle Scholar
Zmuda, J. & Friedenson, B. (1983) Changes in intracellular glutathione levels in stimulated and unstimulated lymphocytes in the presence of 2-mercaptoethanol or cysteine. Journal of Immunology 130, 362364.CrossRefGoogle ScholarPubMed