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The influence of guar gum on intestinal cholesterol transport in the rat

Published online by Cambridge University Press:  09 March 2007

Jennifer M. Gee ,
N. A. Blackburn  and
I. T. Johnson
Jennifer M. Gee
Affiliation:
ARC Food Research Institute, Colney Lane, Norwich NR4 7UA
N. A. Blackburn
Affiliation:
ARC Food Research Institute, Colney Lane, Norwich NR4 7UA
I. T. Johnson
Affiliation:
ARC Food Research Institute, Colney Lane, Norwich NR4 7UA
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Abstract

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1. Everted sacs of rat proximal small intestine were used to determine the effect of guar gum (5 g/l) on the uptake of cholesterol (0·1 mM) from a solution of micelles.

2. The uptake of cholesterol was found to be linear both in the presence and absence of guar gum. When guar was present throughout the whole of the incubation medium, the uptake of cholesterol was reduced to approximately 40% of control values. Sacs which had been pre-incubated in guar gum before exposure to cholesterol in a guar-free medium also showed a reduction in cholesterol uptake but this was less pronounced.

3. A two-stage perfusion technique, previously described (Blackburn & Johnson, 1981), was used to determine the effect of a guar layer adsorbed to the mucosal surface on cholesterol absorption in vivo. Such a layer leads to a reduction of approximately 36%: itwas concluded that guar slows the absorption of cholesterol from micelles by a mechanism, or mechanisms, involving an increased resistance to diffusion in the aqueous medium.

4. Groups of rats were meal-fed for at least 30 d on semi-synthetic diets with or without the inclusion of guar gum (20 g/kg). Rates of intestinal absorption of cholesterol, glucose and fluid were then determined by the perfusion technique in vivo. There was no reduction in absorption in the test animals compared with the controls.

5. It is proposed that guar gum is able to slow the intestinal transport of cholesterol from a suspension of pre-formed micelles, but only when both are present in the lumen together. No evidence was obtained to suggest that the consumption by rats of a diet containing guar gum, at a level similar to that used in human studies, leads to any adaptive reduction in their rates of cholesterol or glucose absorption.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 50 , Issue 2 , September 1983 , pp. 215 - 224
Copyright
Copyright © The Nutrition Society 1983

References

REFERENCES

Anderson, J. W. & Chen, W. (1979). American Journal of Clinical Nutrition 32, 346–363.CrossRefGoogle Scholar
Blackburn, N. A. & Johnson, I. T. (1981). British Journal of Nutrition 46, 239–246.CrossRefGoogle Scholar
Blackburn, N. A., Johnson, I. T. & Gee, J. M. (1982). Gastroenterology 82, 821.CrossRefGoogle Scholar
Chow, S. L. & Hollander, D. (1978). Lipids 13, 239–245.CrossRefGoogle Scholar
Ecknauer, R., Sircar, B. & Johnson, L. R. (1981). Gastroenterology 81, 781–786.CrossRefGoogle Scholar
Jenkins, D. J. A., Reynolds, D., Slavin, B., Leeds, A. R., Jenkins, A. L. & Jepson, E. M. (1980). American Journal of Clinical Nutrition 33, 575–581.CrossRefGoogle Scholar
Johnson, I. T. & Gee, J. M. (1981). Gut 22, 398–403.CrossRefGoogle Scholar
Khan, A. R., Ghazala, Y. K., Mitchel, A. & Qadeer, M. A. (1981). American Journal of Clinical Nutrition 34, 2446–2449.CrossRefGoogle Scholar
Miyamoto, Y., Hanano, M., Iga, T. & Ishikawa, M. (1982). Journal of Pharmacobio-Dynamics 5, 445–447.CrossRefGoogle Scholar
National Research Council (1972). Nutrient Requirements of Laboratory Animals, 2nd ed. p. 64. Washington DC: National Academy of Sciences.Google Scholar
Parsons, D. S. & Wingate, D. L. (1961). Biochimica et Biophysica Acta 46, 170–183.CrossRefGoogle Scholar
Schwartz, S. E. & Levine, G. D. (1980). Gastroenterology 79, 833–836.CrossRefGoogle Scholar
Thomson, A. B. R. (1980). Journal of Lipid Research 21, 687–698.CrossRefGoogle Scholar
Vahouny, G. V., Roy, T., Gallo, L. L., Story, J. A., Kritchevsky, D. & Cassidy, M. (1980 a). American Journal of Clinical Nutrition 33, 2182–2191.CrossRefGoogle Scholar
Vahouny, G. V., Roy, T., Gallo, L. L., Story, J. A., Kritchevsky, D., Cassidy, M., Grund, B. M. & Treadwell, C. R. (1978). American Journal of Clinical Nutrition 31, S208–S212.CrossRefGoogle Scholar
Vahouny, G. V., Tombes, R., Cassidy, M. M., Kritchevsky, D. & Gallo, L. L. (1980 b). Lipids 15, 1012–1018.CrossRefGoogle Scholar
Wilson, J. D., Lindsey, C. A. & Dietschy, J. M. (1968). Annals of the New York Academy of Sciences 149, 808–821.CrossRefGoogle Scholar