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Influence of dietary fat on postprandial glucose metabolism (exogenous and endogenous) using intrinsically 13C-enriched durum wheat

Published online by Cambridge University Press:  09 March 2007

Sylvie Normand ,
Yadh Khalfallah ,
Corinne Louche-Pelissier ,
Christiane Pachiaudi ,
Jean-Michel Antoine ,
Stéphane Blanc ,
Michel Desage ,
Jean Paul Riou  and
Martine Laville
Sylvie Normand*
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France INSERM U449, Faculté de Médecine RTH Laënnec, 8 rue G. Paradin, 69372 Lyon Cedex 08, France
Yadh Khalfallah
Affiliation:
INSERM U449, Faculté de Médecine RTH Laënnec, 8 rue G. Paradin, 69372 Lyon Cedex 08, France
Corinne Louche-Pelissier
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France
Christiane Pachiaudi
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France
Jean-Michel Antoine
Affiliation:
Danone Vitapole, 15 Avenue Galilée-92350-Le Plessis Robinson, France
Stéphane Blanc
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France
Michel Desage
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France
Jean Paul Riou
Affiliation:
INSERM U449, Faculté de Médecine RTH Laënnec, 8 rue G. Paradin, 69372 Lyon Cedex 08, France
Martine Laville
Affiliation:
Human Nutrition Research Centre (HRNC), Batiment 1, Edouard Herriot Hospital, Lyon, France INSERM U449, Faculté de Médecine RTH Laënnec, 8 rue G. Paradin, 69372 Lyon Cedex 08, France
*
*Corresponding author: Dr. S. Normand, fax +33 4 78 77 87 62, email [email protected]
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Abstract

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The present study evaluates the influence of different amounts of fat added to starch on postprandial glucose metabolism (exogenous and endogenous). Nine women (24 (SE 2) YEARS OLD, BMI 20·4 (se 0·7) kg/m2) ingested 1 week apart 75 g glucose equivalent of 13C-labelled starch in the form of pasta without (low fat; LF) or with 15 (medium fat; MF) or 40 (high fat; HF) g sunflower oil. During the 7 h following meal consumption, plasma glucose, non-esterified fatty acids, triacylglycerols (TG) and insulin concentrations, and endogenous (using [6,6-2H2]glucose) and exogenous glucose turnover were determined. With MF and HF meals, a lower postprandial glucose peak was observed, but with a secondary recovery. A decrease in exogenous glucose appearance explained lower glycaemia in HF. At 4 h after the HF meal the insulin, insulin:glucose and postprandial blood TG were higher than those measured after the LF and MF meals. Despite higher insulinaemia, total glucose disappearance was similar and endogenous glucose production was suppressed less than after the LF and MF meals, suggesting insulin resistance. Thus, the addition of a large amount of fat appears to be unfavourable to glucose metabolism because it leads to a feature of insulin resistance. On the contrary, the MF meal did not have these adverse effects, but it was able to decrease the initial glycaemic peak.

Keywords

13C-labelled starchPolyunsaturated fatBioavailabilityPostprandial glucose metabolismOral load
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 1 , July 2001 , pp. 3 - 11
Copyright
Copyright © The Nutrition Society 2001

References

Acheson, KJ, Thelin, A, Ravussin, E, Arnaud, M & Jequier, MD (1985) Contribution of 500 g naturally labelled 13C dextrin maltose to total carbohydrate utilization and the effect of the antecedent diet in man. American Journal of Clinical Nutrition 41, 881–890.CrossRefGoogle ScholarPubMed
Barter, PJ, Nestel, PJ & Carroll, KF (1972) Precursors of plasma triglyceride fatty acid in humans: effects of glucose consumption, clofibrate administration and alcoholic fatty liver. Metabolism 21, 117–124.Google Scholar
Beck-Nielsen, H, Pedersen, O & Sorensen, NS (1978) Effects of diet on the cellular insulin binding and the insulin sensitivity in young healthy subjects. Diabetologia 15, 289–296.CrossRefGoogle ScholarPubMed
Bergmeyer, HU (editor) (1974 a) In Methods of Enzymatic Analysis, 3rd ed., vol. 3, p. 1228D-glucose New York: Acadamic Press.Google Scholar
Bergmeyer, HU (editor) (1974 b) In Methods of Enzymatic Analysis, 3rd ed., vol. 4, p. 1831Triglicerides Ney York: Acadamic Press.Google Scholar
Bevilacqua, S, Buzzigoli, G, Bonadonna, R, Brandi, LS, Oleggini, M, Boni, C, Geloni, M & Ferrannini, E (1990) Operation of Randle's cycle in patients with NIDDM. Diabetes 39, 383–389.CrossRefGoogle ScholarPubMed
Bier, DM, Leake, RD, Haymond, MW, Arnold, KJ, Gruenke, LD, Sperling, MA & Kipnis, DA (1977) Measurement of 'true' glucose production rates in infancy and childhood with 6-6 dideuteroglucose. Diabetes 26, 1016–1023.CrossRefGoogle ScholarPubMed
Binnert, C, Pachiaudi, C, Beylot, M, Croset, M, Cohen, R, Riou, JP & Laville, M (1996) Metabolic fate of an oral long-chain triglyceride load in humans. American Journal of Physiology 270, E445–E450.Google ScholarPubMed
Boden, G, Chen, X, Ruiz, J, White, JV & Rossetti, L (1994) Mechanisms of fatty acid-induced inhibition of glucose uptake. Journal of Clinical Investigation 93, 2438–2446.Google Scholar
Cecil, JE, Francis, J & Read, NW (1999) Comparison of the effects of a high-fat and high-carbohydrate soup delivered orally and intragastrically on gastric emptying. Physiology and Behavior 67, 299–306.CrossRefGoogle ScholarPubMed
Cohen, JC & Berger, GM (1990) Effects of glucose ingestion on post-prandial lipemia and triglyceride clearance in humans. Journal of Lipid Research 31, 597–602.Google Scholar
Cohen, JC, Noakes, TD & Benade, AJ (1988) Serum triglycerides responses to fatty meals: effect of meal fat content. American Journal of Clinical Nutrition 47, 825–827.CrossRefGoogle ScholarPubMed
Collier, GR, Greenberg, GR, Wolever, TMS & Jenkins, DJA (1988) The acute effect of fat on insulin secretion. Journal of Clinical Endocrinology and Metabolism 66, 323–326.CrossRefGoogle ScholarPubMed
Collier, G, McLean, A & O'Dea, K (1984) Effect of co-ingestion of fat on the metabolic response to slowly and rapidly absorbed carbohydrates. Diabetologia 26, 50–54.Google Scholar
Collier, GR, Wolever, TH & Jenkins, DJA (1987) Concurrent ingestion of fat and reduction in starch content impairs carbohydrate tolerance to subsequent meals. American Journal of Clinical Nutrition 45, 963–969.Google Scholar
Cunningham, KM & Read, NW (1989) The effect of incorporating fat into different components of a meal on gastric emptying and postprandial blood glucose and insulin responses. British Journal of Nutrition 61, 285–290.CrossRefGoogle ScholarPubMed
De Bodo, RC, Steele, R, Altszuler, N, Dunn, A & Bishop, JS (1963) On the hormonal regulation of carbohydrate metabolism: studies with 14C glucose. Recent Progress in Hormone Research 19, 445–448.Google Scholar
Dejongh, DC (1965) Mass spectrometry in carbohydrate chemistry. A cyclic peracetates of pentoses and hexoses. Journal of American Chemical Society 30, 453–458.Google Scholar
Dubois, C, Armand, M, Azais-Braesco, V, Portugal, H, Pauli, AM, Bernard, PM, Latge, C, Lafont, H, Borel, P & Lairon, D (1994) Effects of moderate amounts of emulsified dietary fat on postprandial lipemia and lipoproteins in normolipidic adults. American Journal of Clinical Nutrition 60, 374–382.Google Scholar
Dubois, C, Beaumier, G, Juhel, C, Armand, M, Portugal, H, Pauli, AM, Borel, P, Latge, C & Lairon, D (1998) Effects of graded amounts (0–50g) of dietary fat on postprandial lipemia and lipoproteins in normolipidic adults. American Journal of Clinical Nutrition 67, 31–38.CrossRefGoogle Scholar
Estrich, D, Ravnik, A, Schlierf, G, Fukayama, G & Kinsell, L (1967) Effects of coingestion of fat and protein upon carbohydrate induced hyperglycemia. Diabetes 16, 232–237.Google Scholar
Ferrannini, E, Barrett, EJ, Bevilacqua, S & DeFronzo, RA (1983) Effect of free fatty acids on glucose production and utilization in man. Journal of Clinical Investigation 72, 1737–1747.Google Scholar
Ferrannini, E, Bjorkman, O, Reichard, GA, Alessandro, JR, Olsson, M, Wahren, J & DeFronzo, RA (1985) The disposal of an oral glucose load in healthy subjects. Diabetes 34, 580–588.CrossRefGoogle ScholarPubMed
Ferrannini, E, Wahren, J, Felig, P & DeFronzo, RA (1980) The role of fractional glucose extraction in the regulation of splanchnic glucose metabolism in normal and diabetic man. Metabolism 29, 28–35.CrossRefGoogle ScholarPubMed
Flatt, JP, Ravussin, E, Achreson, KJ & Jequier, E (1985) Effects of dietary fat on postprandial substrate oxidation and on carbohydrate and fat balances. Journal of Clinical Investigation 76, 1019–1024.Google Scholar
Fraze, E, Donner, CC, Swislocki, ALM, Chiou, YAM, Chen, YDI & Reaven, GM (1985) Ambient plasma free fatty acid concentrations in noninsulin-dependent diabetes mellitus: evidence for insulin resistance. Journal of Clinical Endocrinology and Metabolism 61, 807–811.Google Scholar
Gannon, MC, Nuttall, FQ, Westphal, SA & Seaquist, ER (1993) The effect of fat and carbohydrate on plasma glucose, insulin, C-peptide, and triglycerides in normal male subjects. Journal of the American College of Nutrition 12, 36–41.CrossRefGoogle ScholarPubMed
Gatti, E, Noe, D, Pazzucconi, F, Gianfranceschi, G, Porrini, M, Testolin, G & Sirtori, CR (1992) Differential effect of unsaturated oils and butter on blood glucose and insulin response to carbohydrate in normal volunteers. European Journal of Clinical Nutrition 46, 161–166.Google Scholar
Griffiths, AJ, Humphreys, SM, Clark, ML, Fielding, BA & Frayn, KN (1994) Immediate metabolic availability of dietary fat in combination with carbohydrate. American Journal of Clinical Nutrition 59, 53–59.CrossRefGoogle ScholarPubMed
Gulliford, MC, Bicknell, EJ & Scarpello, JH (1989) Differential effect of protein and fat ingestion on blood glucose responses to high-and low-glycemic-index carbohydrates in non-insulin-dependant diabetic subjects. American Journal of Clinical Nutrition 50, 773–777.Google Scholar
Havel, RJ, Kane, JP, Balasse, O, Segel, N & Basso, LV (1970) Splanchnic metabolism of free fatty acids and the production of triglycerides of VLDL in normotriglyceridemic and hypertri-glyceridemic humans. Journal of Clinical Investigation 49, 2017–2035.Google Scholar
Himaya, A, Fantino, M, Antoine, JM, Brondel, L & Louis-Sylvestre, J (1997) The satiety power of dietary fat: a new appraisal. American Journal of Clinical Nutrition 65, 1410–1418.Google Scholar
Himsworth, HP (1935) The dietetic factor determining the glucose tolerance and sensitivity to insulin in healthy men. Clinical Science 2, 67–94.Google Scholar
Houghton, LA, Mangnall, YF & Read, NW (1990) Effect of incorporating fat into a liquid test meal on the relation between intragastric distribution and gastric emptying in human volunteers. Gut 31, 1226–1229.Google Scholar
Jackson, RA, Roshania, RD & Hava, MI (1986) Impact of glucose ingestion on hepatic and peripheral glucose metabolism in man: an analysis based on simultaneous use of the forearm and double isotope techniques. Journal of Clinical Endocrinology and Metabolism 63, 541–549.CrossRefGoogle Scholar
Jones, P, Pencharz, P & Clandinin, M (1985) Absorption of 13C-labeled stearic, oleic and linoleic acids in humans: application to breath tests. Journal of Laboratory and Clinical Medicine 105, 647–652.Google Scholar
Kelley, D, Mitrakou, A, Marsh, H, Schwenk, F, Benn, J, Sonnenberg, G, Arcangeli, M, Aoki, T, Sorensen, J, Berger, M, Sonksen, P & Gerich, J (1988) Skeletal muscle glycolysis, oxidation and storage of oral glucose load. Journal of Clinical Investigation 81, 1563–1571.CrossRefGoogle ScholarPubMed
Latgé, C, Thouvenot, P & Kedzierewicz, F (1994) The influence of a lipid loading on gastric emptying and glycemia. American Journal of Clinical Nutrition 59 Suppl., S782.Google Scholar
Laville, M, Riou, JP, Beylot, M, Perrot, L, Odeon, M, Berthezene, F & Mornex, R (1983) Mesure des acides gras libres par méthode enzymatique (Measurement of free fatty acids by enzymic method). Lyon Medical 249, 67–70.Google Scholar
Normand, S, Pachiaudi, C, Khalfallah, Y, Guilluy, R, Mornex, R & Riou, JP (1992) 13C appearance in plasma glucose and breath CO2 during feeding with naturally 13C-enriched starchy food in normal humans. American Journal of Clinical Nutrition 55, 430–435.Google Scholar
Olefsky, JM, Farquar, JW & Reaven, GM (1974) Reappraisal of the role of insulin in hypertriglyceridemia. American Journal of Medicine 57, 551–560.CrossRefGoogle ScholarPubMed
Proietto, J, Rohner-Jeannenaud, F, Ionescu, E & Terretaz, J (1987) Non steady state measurement of glucose turnover in rats by a one-compartment model. American Journal of Physiology 252, E77–E84.Google Scholar
Rasmussen, O (1993) Dose-dependency of the glycemic response to starch-rich meals in insulin-dependant diabetic subjects: studies at constant insulinemia. Metabolism 42, 710–713.CrossRefGoogle Scholar
Rasmussen, O, Lauszus, FF, Christiansen, C, Thomsen, C & Hermansen, K (1996) Differential effects of saturated and monounsaturated fat on blood glucose and insulin responses in subjects with NIDDM. American Journal of Clinical Nutrition 63, 249–253.Google Scholar
Reaven, GM (1988) Role of insulin resistance in human disease. Diabetes 37, 1595–1607.Google Scholar
Thivend, P, Mercier, C, Guilbot, A & Whisler, RL (1972) Determination of starch with glucoamylase. with glucoamylase. In Methods in Carbohydrate Chemistry, vol. 6, pp. 100–105 [Whisler, RL, editor]. New York and London: Academic Press.Google Scholar
Tissot, S, Normand, S, Guilluy, R, Pachiaudi, C, Beylot, M, Laville, M, Cohen, R, Mornex, R & Riou, JP (1990) Use of a new gas chromatograph isotope ratio mass spectrometer to trace the exogenous 13C labelled glucose at a very low level of enrichment in man. Diabetologia 33, 449–456.Google Scholar
Tobey, TA, Greenfield, M, Kramer, F & Reaven, GM (1981) Relationship between insulin resistance, insulin secretion, VLDL kinetics, and plasma TG levels in normotriglyceridemic man. Metabolism 30, 165–171.Google Scholar
Van Amelsvoort, JMM, Van Stratum, P, Kraal, JH, Lussenburg, RN & Dubbelman, GP (1990) Minor difference in postprandial responses of men between starch and sugar when replacing fat in a normal meal. British Journal of Nutrition 63, 37–51.CrossRefGoogle Scholar
Van Amelsvoort, JMM, Van Stratum, P, Kraal, JH, Lussenburg, RN & Houtsmuller, UMT (1989) Effect of varying the carbohydrate:fat ratio in hot lunch on postprandial variables in male volunteers. British Journal of Nutrition 61, 267–283.Google Scholar
Wolever, TM (2000) Dietary carbohydrates and insulin action in humans. British Journal of Nutrition 83 (Suppl. 1), S97–S102.CrossRefGoogle ScholarPubMed