Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-18T18:01:16.411Z Has data issue: false hasContentIssue false
  • English
  • Français

Influence of Camembert consumption on the composition and metabolism of intestinal microbiota: a study in human microbiota-associated rats

Published online by Cambridge University Press:  09 March 2007

Christophe Lay ,
Malène Sutren ,
Pascale Lepercq ,
Catherine Juste ,
Lionel Rigottier-Gois ,
Evelyne Lhoste ,
Riwanon Lemée ,
Pascale Le Ruyet ,
Joël Doré  and
Claude Andrieux
Christophe Lay
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Malène Sutren
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Pascale Lepercq
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Catherine Juste
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Lionel Rigottier-Gois
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Evelyne Lhoste
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Riwanon Lemée
Affiliation:
Lactalis Recherche et Développement, 53089 Laval Cedex, France
Pascale Le Ruyet
Affiliation:
Lactalis Recherche et Développement, 53089 Laval Cedex, France
Joël Doré
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
Claude Andrieux*
Affiliation:
UEPSD, Centre de Recherche de Jouy en Josas, INRA, 78352 Jouy en Josas Cedex, France
*
*Corresponding author: fax +33 1 34 652492, Email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The objective of the present study was to evaluate the consequence of Camembert consumption on the composition and metabolism of human intestinal microbiota. Camembert cheese was compared with milk fermented by yoghurt starters and Lactobacillus casei as a probiotic reference. The experimental model was the human microbiota-associated (HM) rat. HM rats were fed a basal diet (HMB group), a diet containing Camembert made from pasteurised milk (HMCp group) or a diet containing fermented milk (HMfm group). The level of micro-organisms from dairy products was measured in faeces using cultures on a specific medium and PCR–temporal temperature gradient gel electrophoresis. The metabolic characteristics of the caecal microbiota were also studied: SCFA, NH3, glycosidase and reductase activities, and bile acid degradations. The results showed that micro-organisms from cheese comprised 105–108 bacteria/g faecal sample in the HMCp group. Lactobacillus species from fermented milk were detected in HMfm rats. Consumption of cheese and fermented milk led to similar changes in bacterial metabolism: a decrease in azoreductase activity and NH3 concentration and an increase in mucolytic activities. However, specific changes were observed: in HMCp rats, the proportion of ursodeoxycholic resulting from chenodeoxycholic epimerisation was higher; in HMfm rats, α and β-galactosidases were higher than in other groups and both azoreductases and nitrate reductases were lower. The results show that, as for fermented milk, Camembert consumption did not greatly modify the microbiota profile or its major metabolic activities. Ingested micro-organisms were able to survive in part during intestinal transit. These dairy products exert a potentially beneficial influence on intestinal metabolism.

Keywords

CheeseCamembertHuman microbiota-associated ratsIntestinal microbiotaProbiotics
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 429 - 438
Copyright
Copyright © The Nutrition Society 2004

References

Andrieux, C, Lory, S, Dufour-Lescoat, Cde Baynast, R & Szylit, OPhysiological effects of inulin in germ-free rats and in heteroxenic rats inoculated with a human flora. Food Hydrocolloid (1991) 5, 49–56CrossRefGoogle Scholar
Andrieux, C, Membre, JM, Cayuela, C & Antoine, JMMetabolic characteristics of the fecal microflora in humans from three age groups. Scand J Gastroenterol (2002) 7, 792–798Google Scholar
Bartram, HP, Sheppach, W, Gerlach, SRuckdeschel, G, Kelber, E & Kasper, HDoes yogurt enriched with Bifidobacterium longum affect colonic microbiology and fecal metabolism in healthy subjects?. Am J Clin Nutr (1994) 59, 428–432Google Scholar
Bezkorovainy, AProbiotics: determinants of survival and growth in the gut. Am J Clin Nutr (2001) 73, 399S–405SGoogle Scholar
Blaut, M, Collins, MD, Welling, GW, Doré, Jvan Loo, J & de Vos, WMolecular biological methods for studying the gut microbiota: the EU human gut flora project. Br J Nutr (2002) 87, S203–S211CrossRefGoogle ScholarPubMed
Cohen, BI, Raicht, RF, Deschner, EETakahashi, M, Sarwal, AN & Fazzini, EEffect of cholic acid feeeding on N -methyl- N -nitrosourea-induced colon tumors and cell kinetics in rats. J Natl Cancer Inst (1980) 64, 573–578Google Scholar
De Roos, NM & Katan, MBEffects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers between 1988 and 1998. Am J Clin Nutr (2000) 71, 405–411CrossRefGoogle ScholarPubMed
Djouzi, Z, Andrieux, C, Degivry, MC, Bouley, C & Szylit, OThe association of yogurt starters with Lactobacillus casei DN 114.001 in fermented milk alters the composition and metabolism of intestinal microflora in Germ-free rats and in human flora-associated rats. J Nutr (1997) 127, 2260–2266Google Scholar
Drospy, G & Boy, JDétermination de l'ammoniémie (méthode automatique par dialyse) (Determination of ammonia in the blood (automatic method using dialysis). Ann Biol Clin (Paris) (1961) 19, 313–318Google Scholar
Earnest, DL, Holubec, H, Wali, RK, Jolley, CS, Bissonette, M, Bhattacharyya, AK, Roy, H, Khare, S & Brasitus, TAChemoprevention of azoxymethane-induced colonic carcinogenesis by supplemented dietary ursodeoxycholic acid. Cancer Res (1994) 54,5071–5074Google Scholar
Godon, JJ, Zumstein, E, Dabert, P, Habouzit, F & Moletta, RDiversity of microbial genes encoding small-subunit rRNA in an anerobic digestor. Appl Environ Microbiol (1997) 63, 2802–2813CrossRefGoogle Scholar
Goldin, BRHealth benefits of probiotics. Br J Nutr (1998) 80, S203–S207Google Scholar
Guérin-Danan, C, Chabanet, C, Pedone, C, Popot, F, Vaissade, P, Bouley, C, Szylit, O & Andrieux, CMilk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk: influence on intestinal microflora in healthy infants. Am J Clin Nutr (1998) 67, 111 –117Google Scholar
Heyman, M & Ménard, SProbiotic microorganisms: how they affect intestinal pathophysiology. Cell Mol Life Sci (2002) 59, 1151–1165.Google Scholar
Lenoir, J, Lamberet, G & Schmidt, JLL'élaboration d'un fromage: l'exemple du Camembert (Cheese making: example of Camembert). Pour la Science, Dossier hors série, March, (1995) pp. 64–73Google Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJProtein measurement with the Folin-phenol reagent. J Biol Chem (1951) 193, 265–275Google Scholar
Macfarlane, GT & Cummings, JH (1991) The colonic flora, fermentation and large bowel digestive function. In The Large Intestine: Physiology, Pathophysiology and Disease, pp 51–92 [Phillips, SF, Pemberton, JH & Shorter, RG editors]. New York: Raven Press.Google Scholar
Marteau, Ph, Pochard, Ph, Bouhnik, Y & Rambaud, JCSurvie et effets de lactobacilles acidophiles et bifidobactéries de produits laitiers fermentés dans le tube digestif de l'homme (Survival and effects of acidophilic lactobacilli and bifidobacteria from fermented milk products in the human digestive tract). Cah Nutr Diét (1994) 6, 336–340Google Scholar
Marteau, P, Seksik, P & Jian, RProbiotics and intestinal health effects: a clinical perspective. Br J Nutr (2002) 88, S51–S57.Google Scholar
Martinez, JD, Stratagoules, ED, LaRue, JM, Powell, AA, Gause, PR, Craven, MT, Payne, CM, Powell, MBGerner, EW & Earnest, DLDifferent bile acids exhibit distinct biological effects: the tumor promoter deoxycholic acid induces apoptosis and the chemopreventive agent ursodeoxycholic acid inhibits cell proliferation. Nutr Cancer (1998) 3, 111–118Google Scholar
Milkiewicz, P, Roma, MG, Elias, E & Coleman, RHepatoprotection with tauroursodeoxycholate and betammuricholate against taurolithocholate induced cholestasis: involvement of signal transduction pathways. Gut 2002) 51, 113–119Google Scholar
Milovic, V, Teller, IC, Murphy, GM, Caspary, WF & Stein, JDeoxycholic acid stimulates migration in colon cancer cells. Eur J Gastroenterol Hepatol (2001) 13, 945–949CrossRefGoogle ScholarPubMed
Reid, GThe scientific basis for probiotics strains of Lactobacillus. Appl Environ Microbiol (1999) 65, 3763–3768Google Scholar
Rumney, CJ & Rowland, IRIn vivo and in vitro models of the human colonic flora. Crit Rev Food Sci Nutr (1992) 31, 299–331CrossRefGoogle ScholarPubMed
Satokari, RM, Vaughan, EE, kkermans, ADL, Saarela, M & De Vos, WMBifidobacterial diversity in human feces detected by genus-specific PCR and denaturating gradiant gel electrophoresis. Appl Environ Microbiol (2001) 67, 504–513Google Scholar
Salminen, S, Bouley, C, Boutron-Ruault, MC, Cummins, JH, Franck, A, Gibson, GR, Isoluari, E, Moreau, MC, Roberfroid, M & Rowland, IFunctional food science and gastrointestinal physiology and function. Br J Nutr (1998) 80, S147–S171Google Scholar
Sanders, MESummary of conclusions from a consensus panel of experts on health attributes of lactic cultures: significance to fluid milk products containing cultures. J Dairy Sci (1993) 76, 1819–1828Google Scholar
Seksik, P, Rigottier-Gois, L, Gramet, G, Sutren, M, Pochart, P, Marteau, P, Jian, R & Doré, JAlterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon. Gut (2003) 52, 237–242Google Scholar
Stanton, C, Gardiner, G, Lynch, PB, Collins, JK, Fitzgerald, G & Ross, RPProbiotic cheese. Int Dairy J (1998) 8, 491–496Google Scholar
Suau, A, Bonnet, R, Sutren, M, Godon, JJ, Gibson, GR, Collins, MD & Doré, JDirect analysis of genes endocoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol (1999) 65, 4799–4807CrossRefGoogle ScholarPubMed
Tannock, GW, Munro, K, Harmsen, HJM, Welling, GM, Smart, J & Gopal, PKAnalysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl Environ Microbiol (2000) 66, 2578–2588Google Scholar
Walter, J, Hertel, C, Tannock, GW, Lis, CM, Munro, K & Hammes, WPDetection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-pecific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol (2001) 67, 2578–2585CrossRefGoogle ScholarPubMed
Zoetendal, EG, Akkermans, ADL & De Vos, WM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and hosts specific communities of active bacteria. Appl Environ Microbiol 64, 3854–3859Google Scholar