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Influence of antibiotics and food intake on liver glutathione and cytochrome P-450 in septic rats

Published online by Cambridge University Press:  09 March 2007

Virginie Colomb ,
Jean Petit ,
Hélène Matheix-Fortunet ,
Bernadette Hecketsweiler ,
Nathalie Kaeffer ,
Eric Lerebours ,
Raymond Colin  and
Jean-François Lemeland
Virginie Colomb
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Jean Petit
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Hélène Matheix-Fortunet
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Bernadette Hecketsweiler
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Nathalie Kaeffer
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Eric Lerebours
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Raymond Colin
Affiliation:
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Université de Rouen, Hôpital Charles Nicolle, 76031, Rouen-Cédex, France
Jean-François Lemeland
Affiliation:
Laboratoire de Bactériologie, Université de Rouen, Hôpital Charles Nicolle, 76031 Rouen-Cédex, France
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Abstract

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Experimental sepsis in rats induces a restriction in spontaneous food intake and a drop in liver glutathione, cytochrome P-450 (P-450) and aminopyrine demethylase (AD) activity. The present study was designed to assess the effects of antibiotics alone or when combined with food deprivation on these variables. Eighty-nine male Sprague-Dawley rats were assigned to six groups: control (C), acute infection (experimental pyelonephritis, I), acute infection with antibiotics and food given ad lib. (IA), control with antibiotics (CA), acute infection with antibiotics pair-fed to I (IAR), and sham-operated pair-fed to I (SR). Liver glutathione, P-450 and AD activities were reduced by 45·2, 79·8 and 41·2% respectively in group I. Glutathione and AD significantly increased only in those infected rats given antibiotics and allowed free access to food. P-450 did not normalize within the study period in infected rats receiving antibiotics and food repletion. The risk of drug hepatotoxicity in acute septic states is therefore closely related to the nutritional status. From this point of view, nutritional support is almost as important as treatment of infection.

Keywords

Experimental sepsisGlutathioneCytochrome P-450Rat
Type
Immunity, nutrition and growth performance
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 99 - 110
Copyright
Copyright © The Nutrition Society 1995

References

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