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Incorporation of carotenoids from paprika oleoresin into human chylomicrons

Published online by Cambridge University Press:  09 March 2007

Antonio Pérez-Gálvez ,
Hans D. Martin ,
Helmut Sies  and
Wilhelm Stahl
Antonio Pérez-Gálvez
Affiliation:
Institute for Physiological Chemistry I, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany
Hans D. Martin
Affiliation:
Institute for Organic Chemistry I, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany
Helmut Sies
Affiliation:
Institute for Physiological Chemistry I, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany
Wilhelm Stahl*
Affiliation:
Institute for Physiological Chemistry I, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany
*
*Corresponding author: Professor Wilhelm Stahl, fax +49 211 811 3029, email [email protected]
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Abstract

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The intake of a carotenoid-rich diet is epidemiologically related to a lower risk for different chronic disorders like cardiovascular disease, some types of cancer or age-related macular degeneration. Red pepper (Capsicum annuum L.) and its dietary products contain a variety of carotenoids, which may contribute to the carotenoid pattern of human blood and tissues. The objective of the present study was to assess the availability of carotenoids from paprika oleoresin, including zeaxanthin, β-cryptoxanthin, β-carotene and the paprika-specific oxocarotenoids capsanthin and capsorubin. After overnight fasting, the volunteers (n 9) ingested a single dose of the paprika oleoresin containing 6·4 mg zeaxanthin, 4·2 mg β-cryptoxanthin, 6·2 mg β-carotene, 35·0 mg capsanthin and 2·0 mg capsorubin. At different time points the carotenoid pattern in the chylomicron fraction was analysed to evaluate carotenoid absorption. From the major carotenoids present in the paprika oleoresin only zeaxanthin, β-cryptoxanthin and β-carotene were detectable in considerable amounts. Although the xanthophylls in paprika oleoresin were mainly present as mono- or di-esters, only free zeaxanthin and β-cryptoxanthin were found in human samples. The bioavailability of the pepper-specific carotenoids capsanthin and capsorubin from paprika oleoresin is very low. However, oleoresin is a suitable source for the provitamin A carotenoids β-carotene and β-cryptoxanthin and the macular pigment zeaxanthin.

Keywords

BioavailabilityPaprika carotenoidsXanthophyll esters
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 6 , June 2003 , pp. 787 - 793
Copyright
Copyright © The Nutrition Society 2003

References

Barua, AB (1999) Intestinal absorption of epoxy-beta-carotenes by humans. Biochemical Journal 339, 359–362.CrossRefGoogle ScholarPubMed
Beutner, S, Bloedom, B, Frixel, S, Hernandez-Blanco, I, Hoffmann, T, Martin, HD, Mayer, B, Noack, P, Ruck, C, Schmidt, M, Schulke, I, Sell, S, Ernst, H, Haremza, S, Seybold, G, Sies, H, Stahl, W & Walsh, R (2001) Quantitative assessment of antioxidant properties of natural colorants and phytochemicals: carotenoids, flavonoids, phenols and indigoids. The role of β-carotene in antioxidant functions. Journal of the Science of Food and Agriculture 81, 559–568.CrossRefGoogle Scholar
Breithaupt, DE, Bamedi, A & Wirt, U (2002) Carotenol fatty acid esters: easy substrates for digestive enzymes? Comparative Biochemistry and Physiology B 132, 721–728.CrossRefGoogle ScholarPubMed
Britton, G (1995) Structure and properties of carotenoids in relation to function. FASEB Journal 9, 1551–1558.CrossRefGoogle ScholarPubMed
Camara, B & Moneger, R (1978) Free and esterified carotenoids in green and red fruits of Capsicum annuum. Phytochemistry 17, 91–93.CrossRefGoogle Scholar
Curl, AL (1962) The carotenoids of red bell pepper. Journal of Agricultural and Food Chemistry 10, 522–524.CrossRefGoogle Scholar
Davies, BH, Matthews, S & Kirk, JTO (1970) The nature and biosynthesis of the carotenoids of different color varieties of Capsicum annuum. Phytochemistry 9, 797–805.CrossRefGoogle Scholar
Eugster, CH (1995) Chemical derivatization: microscale tests for the presence of common functional groups in carotenoids. In Carotenoids: Vol. 1A, Isolation and Analysis, pp. 76–77. [Britton, G, Liaaen-Jensen, S & Pfander, H, editors]. Basel: Birkhäuser-Verlag.Google Scholar
Gärtner, C, Stahl, W & Sies, H (1996) Preferential increase in chylomicron levels of the xanthophylls lutein and zeaxanthin compared to β-carotene in the human. International Journal of Vitamin and Nutrition Research 66, 119–125.Google ScholarPubMed
Govindarajan, VS (1986) Capsicum – Production, technology, chemistry, and quality – part II. Processed products, standards, world production and trade. Critical Reviews in Food Science and Nutrition 23, 207–288.CrossRefGoogle ScholarPubMed
Granado, F, Olmedilla, B, Gil-Martinez, E & Blanco, I (1998) Lutein ester in serum after lutein supplementation in human subjects. British Journal of Nutrition 80, 445–449.CrossRefGoogle ScholarPubMed
King, TJ, Khachik, F, Bortkiewicz, H, Fukushima, LH, Morioka, S & Bertram, JS (1997) Metabolites of dietary carotenoids as potential cancer preventives agents. Pure and Applied Chemistry 69, 2135–2140.CrossRefGoogle Scholar
Mares-Perlman, JA, Millen, AE, Ficek, TL & Hankinson, SE (2002) The body evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview. Journal of Nutrition 132, 518S–524S.CrossRefGoogle ScholarPubMed
Martin, HD, Jager, C, Ruck, C, Schmidt, M, Walsh, R & Paust, J (1999) Anti- and prooxidant properties of carotenoids. Journal für Praktische Chemie 341, 302–308.3.0.CO;2-6>CrossRefGoogle Scholar
Mínguez-Mosquera, MI & Hornero-Méndez, D (1993) Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika, and oleoresin by reversed-phase HPLC. Journal of Agricultural and Food Chemistry 41, 1616–1620.CrossRefGoogle Scholar
Mínguez-Mosquera, MI & Hornero-Méndez, D (1994) Changes in carotenoid esterification during the fruit ripening of Capsicum annuum Cv Bola. Journal of Agricultural and Food Chemistry 42, 640–644.CrossRefGoogle Scholar
Mínguez-Mosquera, MI & Pérez-Gálvez, A (1998 a) Color quality in paprika oleoresins. Journal of Agricultural and Food Chemistry 46, 5124–5127.CrossRefGoogle Scholar
Mínguez-Mosquera, MI & Pérez-Gálvez, A (1998 b) Study of lability and kinetics of the main carotenoid pigments in the de-esterification reaction. Journal of Agricultural and Food Chemistry 46, 566–569.CrossRefGoogle Scholar
Oshima, S, Ojima, F, Sakamoto, H, Ishiguro, Y & Terao, J (1997) Accumulation and clearance of capsanthin in blood plasma after the ingestion of paprika juice in men. Journal of Nutrition 127, 1475–1479.CrossRefGoogle ScholarPubMed
Parker, RS (1997) Bioavailability of carotenoids. European Journal of Clinical Nutrition 51, S86–S90.Google ScholarPubMed
Pérez-Gálvez, A & Mínguez-Mosquera, MI (2002) Degradation of non-esterified and esterified xanthophylls by free radicals. Biochimica et Biophysica Acta 1569, 31–34.CrossRefGoogle ScholarPubMed
Philip, T, Nawar, WW & Francis, FJ (1971) The nature of the acids and capsanthin esters in paprika. Journal of Food Science 36, 98–102.CrossRefGoogle Scholar
Stahl, W, Ale-Agha, N & Polidori, MC (2002 a) Non-antioxidant properties of carotenoids. Biological Chemistry 383, 553–558.CrossRefGoogle ScholarPubMed
Stahl, W, van den Berg, H, Arthur, J, Bast, A, Dainty, J, Faulks, RM, Gärtner, C, Haenen, G, Hollman, P, Holst, B, Kelly, FJ, Polidori, MC, Rice-Evans, C, Southon, S, van Vliet, T, Viña-Ribes, J, Williamson, G & Astley, SB (2002 b) Bioavailability and metabolism. Molecular Aspects of Medicine 23, 39–100.CrossRefGoogle ScholarPubMed
Terao, J (1989) Antioxidant activity of β-carotene-related carotenoids in solutions. Lipids 24, 659–661.CrossRefGoogle Scholar
Terpstra, AHM (1985) Isolation of serum chylomicrons prior to density gradient ultracentrifugation of other serum lipoprotein classes. Analytical Biochemistry 150, 221–227.CrossRefGoogle ScholarPubMed
van den Berg, H & Van Vliet, T (1998) Effect of simultaneous, single oral doses of β-carotene with lutein or lycopene on the β-carotene and retinyl ester responses in the triacylglycerol-rich lipoprotein fraction of men. American Journal of Clinical Nutrition 68, 82–89.CrossRefGoogle ScholarPubMed
van Het Hof, KH, West, CE, Weststrate, JA & Hautvast, JG (2000) Dietary factors that affect the bioavailability of carotenoids. Journal of Nutrition 130, 503–506.CrossRefGoogle ScholarPubMed
Wingerath, T, Stahl, W & Sies, H (1995) β-Cryptoxanthin selectively increases in human chylomicrons upon ingestion of tangerine concentrate rich in β-cryptoxanthin esters. Archives of Biochemistry and Biophysics 324, 385–390.CrossRefGoogle Scholar