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In vitro Na+, K+-ATPase (EC 3.6.1.3)-dependent respiration and protein synthesis in skeletal muscle of pigs fed at three dietary protein levels

Published online by Cambridge University Press:  09 March 2007

O. Adeola ,
L. G. Young ,
B. W. Mcbride  and
R. O. Ball
O. Adeola
Affiliation:
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
L. G. Young
Affiliation:
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
B. W. Mcbride
Affiliation:
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
R. O. Ball
Affiliation:
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Abstract

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1. Eighteen pigs were offered diets containing 130, 170 or 210 g protein/kg with three barrows and three gilts per diet from 20 to 60 kg live weight. Oxygen consumption, Na1, K1-ATPase (EC 3·6·1· 3)-dependent and -independent respiration and protein synthesis were measured in vitro in intercostal and sartorius muscle preparations from these pigs.

2. Increasing dietary protein concentration increased (P < 0·01) daily gain and dissectible muscle in carcass.

3. O2 consumption and Na+, K+-ATPase-dependent respiration of the intercostal and sartorius muscles increased linearly (P < 0·01) with increase in dietary protein concentration. The requirement for the support of the transport of Na+ and K+ across the cell membrane in these muscles, on average, accounted for 22–25% of the O2 consumption.

4. Synthesis rate (mg/g per d) of protein in the sartorius muscle increased (P < 0·05) from 3·05 to 5·07 and increased (P < 0·1) from 2·57 to 4.06 in the intercostal muscle as dietary protein increased from 130 to 210 g/kg diet.

5. Regression of Na+, K+-ATPase-dependent respiration against protein synthesis in each of intercostal and sartorius muscles showed a linear relation, an attestation of a close link between productive processes and auxiliary energy expenditure.

Type
Energy Metabolism
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 453 - 465
Copyright
Copyright © The Nutrition Society 1989

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