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Impairment of cysteine synthesis from methionine in rats exposed to surgical stress

Published online by Cambridge University Press:  09 March 2007

José Viña
Affiliation:
Departamento de Fisiología, Facultad de Medicina, Av. Blasco Ibañez 17, Valencia-46010, Spain
Angel Gimenez
Affiliation:
Servicio de Cirugía, Hospital de la Marina Alta, Denia, Spain
Inmaculada R. Puertes
Affiliation:
Departamento de Bioquímica y Biologia Molecular, Facultad de Medicina y Farmacia, Av. Blasco Ibañez 15, 17 Valencia-46010, Spain
Esperanza Gasco
Affiliation:
Departamento de Fisiología, Facultad de Medicina, Av. Blasco Ibañez 17, Valencia-46010, Spain
Juan R. Viña
Affiliation:
Departamento de Bioquímica y Biologia Molecular, Facultad de Medicina y Farmacia, Av. Blasco Ibañez 15, 17 Valencia-46010, Spain
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Abstract

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The activity of liver cystathionase (EC 4.4.1.1) was decreased after 3 d of stress induced by surgery. The rate of l-cysteine synthesis from l-methionine was significantly higher in isolated hepatocytes from controls than in hepatocytes from rats suffering from surgical stress. The half-life of l-[2(n)-3H]methionine was significantly higher in rats submitted to surgical stress than in controls. Plasma l-methionine: l-cystine ratio was higher in stressed rats than in controls. l-cystine uptake was significantly increased in the surgically-stressed rats when compared with the controls. All these facts are consistent with the hypothesis that the observed inhibition of cystathionase is physiologically important and thatl-cysteine might be considered as an essential amino acid in cases of surgical stress.

Type
Amino Acid Metabolism
Copyright
Copyright © The Nutrition Society 1992

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