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Impact of exocrine pancreatic adaptation on in vitro protein digestibility

Published online by Cambridge University Press:  09 March 2007

P. Valette ,
H. Malouin ,
T. Corring  and
L. Savoie
P. Valette
Affiliation:
Département de Nutrition humaine et consommation, FSAA, Université Laval, Québec, GIK7P4, Canada
H. Malouin
Affiliation:
Département de Nutrition humaine et consommation, FSAA, Université Laval, Québec, GIK7P4, Canada
T. Corring
Affiliation:
Département de Nutrition humaine et consommation, FSAA, Université Laval, Québec, GIK7P4, Canada
L. Savoie
Affiliation:
Département de Nutrition humaine et consommation, FSAA, Université Laval, Québec, GIK7P4, Canada
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Abstract

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An in vitro enzymic method was used to study the kinetics of digestion of casein and rapeseed proteins. After a predigestion step with pepsin (EC 3.4.23.1), the protein substrates were submitted to a 24 h hydrolysis either with pancreatin or pancreatic juices of pigs adapted either to casein or rapeseed diets and whose enzyme activities were different. After 3, 6 and 24 h of in vitro digestion, dialysates were collected and analysed for content of nitrogen, amino acids and low-molecular-weight peptides. For a long-term hydrolysis (24 h), overall digestibility of both substrates was not affected by the composition of pancreatic enzyme mixtures. However, at the beginning of hydrolysis a significant effect of pancreatic juices was observed, i.e. individual amino acid digestibility was generally higher when casein pancreatic juice was used for hydrolysis and their relative pattern of release was modified. For both substrates the proportion of amino acids released as low-molecular-weight peptides was not affected by the enzyme mixture used and made up about two-thirds of the total digested material. It is concluded that exocrine pancreatic adaptation to protein sources does not affect the total capacity of protein digestion. However, the changes in initial kinetics of release of amino acids are more dependent on the nature of the protein tested than on the composition of pancreatic enzyme mixtures.

Keywords

In vitro protein digestibilityCaseinRapeseedPancreatic juice
Type
Hormonal Effects on Protein Digestibility
Information
British Journal of Nutrition , Volume 69 , Issue 2 , March 1993 , pp. 359 - 369
Copyright
Copyright © The Nutrition Society 1993

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