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Hypoglycaemic and anorexigenic activities of an α-amylase inhibitor from white kidney beans (Phaseolus vulgaris) in Wistar rats

Published online by Cambridge University Press:  09 March 2007

M. A. Tormo ,
I. Gil-Exojo ,
A. Romero de Tejada  and
J. E. Campillo
M. A. Tormo*
Affiliation:
Department of Physiology, Faculty of Medicine, University of Extremadura, Apartado de Correos 108, 06071, Badajoz, Spain
I. Gil-Exojo
Affiliation:
Department of Physiology, Faculty of Medicine, University of Extremadura, Apartado de Correos 108, 06071, Badajoz, Spain
A. Romero de Tejada
Affiliation:
Department of Physiology, Faculty of Medicine, University of Extremadura, Apartado de Correos 108, 06071, Badajoz, Spain
J. E. Campillo
Affiliation:
Department of Physiology, Faculty of Medicine, University of Extremadura, Apartado de Correos 108, 06071, Badajoz, Spain
*
*Corresponding author: Dr M. A. Tormo, fax +34 924 289437, email, [email protected]
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Abstract

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An inhibitor of α-amylase was isolated and purified from an extract of white kidney beans (Phaseolus vulgaris). The acute oral administration of the inhibitor (50 mg/kg body weight) to adult Wistar rats together with a starch load (2 g/kg body weight suspended in NaCl (9 g/l)) reduced the increase in glycaemia over the basal value (NaCl, 222 (SEM 49); inhibitor, 145 (SEM 16) mmol/l×180 min; P<0.05) without modifying the insulin response. On administering the inhibitor orally (50 mg/kg body weight dissolved in NaCl (9 g/l)) for 21 d to rats fed on a standard diet, a decline was observed in the glycaemia values on day 0 (NaCl, 5.53 (SEM 0.12); inhibitor, 5.25 (SEM 0.16) mmol/l) relative to those obtained on days 10 (NaCl, 5.00 (SEM 0.14); inhibitor, 4.60 (SEM 0.08) mmol/l; P<0.05) and 21 (NaCl, 5.22 (SEM 0.22); inhibitor, 4.50 (SEM 0.12) mmol/l; P<0.01) of treatment, without modifying the plasma concentration of insulin. There was found to be a significant anorexigenic action of the inhibitor; there was reduced food intake (NaCl, 23.07 (SEM 0.31); inhibitor, 19.50 (SEM 0.49) g/d; P<0.01), a reduced weight gain (NaCl, 52 (SEM 3); inhibitor, −1.33 (SEM 8.9) g/21 d; P<0.01), as well as changes in the activity of some intestinal enzymes such as maltase (NaCl, 87 (SEM 7); inhibitor, 127 (SEM 11) U/g proteins; P<0.05). The present study has shown, for the first time, that the prolonged administration of an α-amylase inhibitor reduces blood glucose levels and body-weight gain in Wistar rats.

Keywords

α-Amylase inhibitorGlycaemiaWeightFood intakeDisaccharidases
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 5 , November 2004 , pp. 785 - 790
Copyright
Copyright © The Nutrition Society 2004

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