Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-29T16:51:45.233Z Has data issue: false hasContentIssue false
  • English
  • Français

The hydrogenation of some cis- and trans-octadecenoic acids to stearic acid by a rumen Fusocillus sp.

Published online by Cambridge University Press:  09 March 2007

Patrick Kemp ,
David J. Lander  and
Frank D. Gunstone
Patrick Kemp
Affiliation:
AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
David J. Lander
Affiliation:
AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
Frank D. Gunstone
Affiliation:
Department of Chemistry, The University, St Andrews, Fife, Scotland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The hydrogenation of a range of double-bond positional and configurational octadecenoic acid isomers (cis (δ2 and δ4 to δ13) and trans (δ2 and δ5 to δ13)) to stearic acid by a rumen Fusocillus sp. were examined.

2. The cis and trans δS to δ13 isomers were all hydrogenated to some extent by late-log-phase cultures added to suspensions of individual isomers and incubated for a further 3 h. Of the cis-isomers, δ5 to δll (79–73% conversion to stearic acid) were the preferred substrates. δ24s- (30%) and δ13-cis-isomers ( 5 % ) were poorly hydrogenated. Of the trans-isomers, δ8, δ9 and δ10 were 45% converted to stearic acid, the other isomers were poorly hydrogenated. These results are in agreement with less extensive studies using sheep rumen micro-organisms.

3. When cultures were grown from small inocula in media containing individual isomers more extensive hydrogenation was found than with late-log-phase cultures. At 24 h, cis δ2, δ4 and δ5 gave the highest conversions to stearic acid (90%) followed by the cis δ6 to δ12 and trans δ8 to δ10 isomers (approximately 75%), although at 6 and 12 h δ9-trans gave higher yields of stearic acid than δ9-cis, probably because the growth of the cis cultures showed a longer log-phase.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 52 , Issue 1 , July 1984 , pp. 165 - 170
Copyright
Copyright © The Nutrition Society 1984

References

REFERENCES

Body, D. R. (1976). Biochemical Journal 157, 741–744.CrossRefGoogle Scholar
Dawson, R. M. C. & Kemp, P. (1970). In physiology of digestion and metabolism in the Ruminant, pp. 504–518 [Phillipson, A. t., editor] Newcastle upon tyne: Oriel press.Google Scholar
Gunstone, F. D. & Ismail, I. A. (1967 a). Chemistry and Physics of Lipids 1, 209–226.CrossRefGoogle Scholar
Gunstone, F. D. & Ismail, I. A. (1967 b). Chemistry and Physics of Lipids 1, 264–269.Google Scholar
Hazlewood, G. P., Kemp, P., Lander, D. J. & Dawson, R. M. C. (1976). British Journal of Nutrition 35, 293–297.Google Scholar
Hazlewood, G. P., Reynolds, M. J., Dawson, R. M. C. & Gunstone, F. D. (1979). Journal of Applied Bacteriology 47, 321–325.Google Scholar
Hungate, R. E. (1969). In methods in microbiology, vol. 3B, pp.117–132. [Norris, J. R. and Ribbons, D. W., editors]. London: Academic press.Google Scholar
Katz, I. & Keeney, M. (1966). Journal of Dairy Science 49, 962–966.CrossRefGoogle Scholar
Kemp, P., Lander, D. J. & Gunstone, F. D. (1977). Abstracts 11th FEBS Meeting, Copenhagen A5–1 750.Google Scholar
Kemp, P., White, R. W. & Lander, D. J. (1975). Journal of General Microbiology 90, 100–114.Google Scholar
Latham, M. J. & Sharpe, E. M. (1971). In Isolation of Anaerobes, pp. 133–147 [Shapton, D. A. and Board, R. G., editors]. London: Academic Press.Google Scholar
Leat, W. M. F., Kemp, P., Lyons, R. J. & Alexander, T. J. L. (1977). Journal of Agricultural Science, Cambridge 88, 175–179.CrossRefGoogle Scholar
Morris, L. J. (1970). Biochemical Journal 118, 681–693.Google Scholar
Nieman, C. (1954). Bacteriological Reviews 18, 147–167.CrossRefGoogle Scholar
Roché, C., Albertyn, H., van Gylswyk, N. O. & Kestner, A. (1973). Journal of Microbiology 78, 253–260.Google Scholar
von Rudloff, E. (1956). Canadian Journal of Chemistry 34, 1413–1418.Google Scholar