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Glucose partitioning in the pregnant ewe: Effects of undernutrition and exercise

Published online by Cambridge University Press:  09 March 2007

B. J. Leury ,
A. R. Bird ,
K. D. Chandler  and
A. W. Bell
B. J. Leury
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
A. R. Bird
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
K. D. Chandler
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
A. W. Bell
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
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Abstract

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Maternal whole-body glucose entry rate and uterine and umbilical net uptakes of glucose and oxygen were measured in single-pregnant ewes which were either well-fed throughout, or fed at 0.3–0.4 predicted energy requirement for 7–21 d during late pregnancy. All ewes were studied while standing at rest and then while walking on a treadmill at 0.7 m/s on a 10° slope for 60 min. Underfed ewes suffered significant decreases in live weight and had lower fetal, but not placental, weights at 140–144 d gestation. Undernutrition also caused large decreases in maternal glycaemia and glucose entry rate, which were associated with equally large decreases in uterine and umbilical net uptakes and O2 quotients of glucose, and with a decrease in placental glucose transfer capacity. Exercise caused increases in maternal blood concentration, entry rate and uterine net uptake of glucose, the magnitudes of which were not significantly affected by plane of nutrition. Umbilical glucose uptake and placental glucose transfer capacity increased during exercise in underfed but not fed ewes. The fractional distribution of maternal glucose to the pregnant uterus, and of uterine glucose uptake to the fetus, were unaltered by undernutrition; during exercise, a disproportionately small fraction of the increased maternal glucose supply went to the uterus. The results confirm that the ovine conceptus responds to nutritional reduction in maternal glucose availability in a manner similar to non-uterine maternal tissues. Major reductions in glucose supply appear to override putative glucose-sparing mechanisms which may operate to favour the conceptus in better-nourished animals.

Keywords

GlucosePregnancyUterusFetusSheep
Type
Glucose Metabolism
Information
British Journal of Nutrition , Volume 64 , Issue 2 , September 1990 , pp. 449 - 462
Copyright
Copyright © The Nutrition Society 1990

References

Alexander, G. & Williams, D. (1971). Heat stress and development of the conceptus in domestic sheep. Journal of Agricultural Science, Cambridge 76, 53–72.CrossRefGoogle Scholar
Baird, G. D., van der Walt, J. G. & Bergman, E. N. (1983). Whole-body metabolism of glucose and lactate in productive sheep and cows. British Journal of Nutrition 50, 249–265.CrossRefGoogle ScholarPubMed
Barcroft, J. (1946). Researches on Prenatal Life. Oxford: Blackwell.Google Scholar
Bauman, D. E. & Currie, W. B. (1980). Partitioning of nutrients during pregnancy and lactation: a review of mechanisms involving homeostasis and homeorhesis. Journal of Dairy Science 63, 1514–1529.CrossRefGoogle ScholarPubMed
Bell, A. W., Bassett, J. M., Chandler, K. D. & Boston, R. C. (1983). Fetal and maternal endocrine responses to exercise in the pregnant ewe. Journal of Developmental Physiology 5, 129–141.Google ScholarPubMed
Bergman, E. N., Katz, M. L. & Kaufman, C. F. (1970). Quantitative aspects of hepatic and portal glucose metabolism and turnover in sheep. American Journal of Physiology 219, 785–793.CrossRefGoogle ScholarPubMed
Bergmeyer, H. U. & Bernt, E. (1974). d-Glucose. Determination with glucose oxidase and peroxidase. In Methods of Enzymatic Analysis, pp. 1205–1215 [Bergmeyer, H. U., editor]. Weinheim: Verlag Chemie.Google Scholar
Bird, A. R., Chandler, K. D. & Bell, A. W. (1981). Effects of exercise and plane of nutrition on nutrient utilization by the hind limb of the sheep. Australian Journal of Biological Sciences 34, 541–550.CrossRefGoogle ScholarPubMed
Brockman, R. P. (1984). Validation of an equation for calculation of glucose appearance during nonsteady state in sheep. Canadian Journal of Physiology and Pharmacology 62, 341–344.CrossRefGoogle ScholarPubMed
Brockman, R. P. (1987). Effect of exercise on net hepatic uptake of lactate, pyruvate, alanine, and glycerol in sheep. Canadian Journal of Physiology and Pharmacology 65, 2065–2070.CrossRefGoogle ScholarPubMed
Brockman, R. P. & Halvorson, R. (1982). Glucose, glucagon, and insulin during adrenergic blockade in exercising sheep. Journal of Applied Physiology 52, 315–319.CrossRefGoogle ScholarPubMed
Brodie, B. B., Axelrod, J., Soberman, R. & Levy, B. B. (1949). The estimation of antipyrene in biological materials. Journal of Biological Chemistry 197, 25–31.CrossRefGoogle Scholar
Burd, L. T., Jones, M. D., Simmons, M. A., Makowski, E. L., Meschia, G. & Battaglia, F. C. (1975). Placental production and fetal utilization of lactate and pyruvate. Nature 254, 710–711.CrossRefGoogle ScholarPubMed
Chandler, K. D. (1983). Fetal and maternal responses to exercise in the pregnant ewe. M Agr Sc Thesis, La Trobe University.Google Scholar
Chandler, K. D. & Bell, A. W. (1981). Effects of maternal exercise on fetal and maternal respiration and nutrient metabolism in the pregnant ewe. Journal of Developmental Physiology 3, 161–176.Google ScholarPubMed
Chandler, K. D., Leury, B. J., Bird, A. R. & Bell, A. W. (1985). Effects of undernutrition and exercise during late pregnancy on uterine, fetal and uteroplacental metabolism in the ewe. British Journal of Nutrition 53, 625–635.CrossRefGoogle ScholarPubMed
Crandell, S. S., Palma, P. A. & Morriss, F. H. Jr (1983). Umbilical glucose and lactate extractions during maternal hyperglycemia in sheep. American Journal of Physiology 244, R882–R887.Google ScholarPubMed
de Bodo, R. C., Steele, R., Altszuler, N., Dunn, A. & Bishop, J. S. (1963). On the hormonal regulation of carbohydrate metabolism: studies with 14C glucose. Recent Progress in Hormone Research 19, 445–482.Google Scholar
Everitt, G. C. (1968). Prenatal development in uniparous animals, with particular reference to the influence of maternal nutrition in sheep. In Growth and Development of Mammals, pp. 131–157 [Lodge, G. A. and Lamming, G. E., editors]. London: Butterworths.Google Scholar
Faichney, G. J., Barker, P. J., Setchell, B. P. & Lindsay, D. B. (1981). The utilization of lactic acid by sheep in late pregnancy. Quarterly Journal of Experimental Physiology 66, 195–201.CrossRefGoogle ScholarPubMed
Ford, E. J. H. (1962). The effect of dietary restriction on some liver constituents of sheep during late pregnancy and early lactation. Journal of Agricultural Science, Cambridge 59, 67–75.CrossRefGoogle Scholar
Hay, W. W. Jr, Lin, C.-C. & Meznarich, H. K. (1988). Effect of high levels of insulin on glucose utilization and glucose production in pregnant and nonpregnant sheep. Proceedings of the Society for Experimental Biology and Medicine 189, 275–284.CrossRefGoogle ScholarPubMed
Hay, W. W. Jr, Sparks, J. W., Gilbert, M., Battaglia, F. C. & Meschia, G. (1984 a). Effect of insulin on glucose uptake by the maternal hindlimb and uterus, and by the fetus in conscious pregnant sheep. Journal of Endocrinology 100, 119–124.CrossRefGoogle ScholarPubMed
Hay, W. W. Jr, Sparks, J. W., Quissell, B. J., Battaglia, F. C. & Meschia, G. (1981). Simultaneous measurements of umbilical uptake, fetal utilization rate, and fetal turnover rate of glucose. American Journal of Physiology 240, E662–E668.Google ScholarPubMed
Hay, W. W. Jr, Sparks, J. W., Wilkening, R. B., Battaglia, F. C. & Meschia, G. (1983). Partition of maternal glucose production between conceptus and maternal tissues in sheep. American Journal of Physiology 245, E347–E350.Google ScholarPubMed
Hay, W. W. Jr, Sparks, J. W., Wilkening, R. B., Battaglia, F. C. & Meschia, G. (1984 b). Fetal glucose uptake and utilization as functions of maternal glucose concentration. American Journal of Physiology 246, E237–E242.Google ScholarPubMed
Judson, G. J., Filsell, O. H. & Jarrett, I. G. (1976). Glucose and acetate metabolism in sheep at rest and during exercise. Australian Journal of Biological Sciences 29, 215–222.CrossRefGoogle ScholarPubMed
Judson, G. J. & Leng, R. A. (1972). Estimation of the total entry rate and resynthesis of glucose in sheep using glucoses uniformly labelled with 14C and variously labelled with 3H. Australian Journal of Biological Sciences 25, 1313–1332.CrossRefGoogle Scholar
Leury, B. J. (1987). Effects of undernutrition and exercise on glucose metabolism in the pregnant ewe. PhD Thesis, La Trobe University.Google Scholar
Mellor, D. J. (1983). Nutritional and placental determinants of foetal growth rate in sheep and consequences for the newborn lamb. British Veterinary Journal 139, 307–324.CrossRefGoogle ScholarPubMed
Meschia, G., Battaglia, F. C., Hay, W. W. & Sparks, J. W. (1980). Utilization of substrates by the ovine placenta in vivo. Federation Proceedings 39, 245–249.Google ScholarPubMed
Meschia, G., Cotter, J. R., Makowski, E. L. & Barron, D. H. (1967). Simultaneous measurement of uterine and umbilical blood flows and oxygen uptakes. Quarterly Journal of Experimental Physiology 52, 1–18.CrossRefGoogle Scholar
Meschia, G., Makowski, E. L. & Battaglia, F. C. (1969). The use of indwelling catheters in uterine and umbilical veins of sheep for a description of fetal acid-base balance and oxygenation. Yale Journal of Biology and Medicine 42, 154–165.Google ScholarPubMed
Oddy, V. H., Gooden, J. M., Hough, G. M., Teleni, E. & Annison, E. F. (1985). Partitioning of nutrients in Merino ewes. II. Glucose utilization by skeletal muscle, the pregnant uterus and the lactating mammary gland in relation to whole body glucose utilization. Australian Journal of Biological Sciences 38, 95–108.CrossRefGoogle ScholarPubMed
Pethick, D. W., Harman, N. & Chong, J. K. (1987). Non-esterified long-chain fatty acid metabolism in fed sheep at rest and during exercise. Australian Journal of Biological Sciences 40, 221–234.CrossRefGoogle ScholarPubMed
Petterson, J. A., Dunshea, F. R. & Bell, A. W. (1989). Effects of insulin dose and pregnancy on glucose metabolism in euglycemic sheep. Journal of Animal Science 67, Suppl. 1, 201.Google Scholar
Rankin, J. H. G., Jodarski, G. & Shanahan, M. R. (1986). Maternal insulin and placental 3-O-methyl glucose transport. Journal of Developmental Physiology 8, 247–253.Google ScholarPubMed
Simpson, I. A. & Cushman, S. W. (1986). Hormonal regulation of mammalian glucose transport. Annual Reviews of Biochemistry 55, 1059–1089.CrossRefGoogle ScholarPubMed
Somogyi, M. (1945). A new reagent for the estimation of sugars. Journal of Biological Chemistry 160, 61–68.CrossRefGoogle Scholar
Stacey, T. E., Weedon, A. P., Haworth, C., Ward, R. H. T. & Boyd, R. D. H. (1978). Fetomaternal transfer of glucose analogues by sheep placenta. American Journal of Physiology 234, E32–E37.Google ScholarPubMed
Steel, J. W. & Leng, R. A. (1973 a). Effects of plane of nutrition and pregnancy on gluconeogenesis in sheep. 1. The kinetics of glucose metabolism. British Journal of Nutrition 30, 451–473.CrossRefGoogle ScholarPubMed
Steel, J. W. & Leng, R. A. (1973 b). Effects of plane of nutrition and pregnancy on gluconeogenesis in sheep. 2. Synthesis of glucose from ruminal propionate. British Journal of Nutrition 30, 475–489.CrossRefGoogle ScholarPubMed
Wilkening, R. B., Battaglia, F. C. & Meschia, G. (1985). The relationship of umbilical glucose uptake to uterine blood flow. Journal of Developmental Physiology 7, 313–319.Google ScholarPubMed
Wilson, S., MacRae, J. C. & Buttery, P. J. (1983). Glucose production and utilization in non-pregnant, pregnant and lactating ewes. British Journal of Nutrition 50, 303–316.CrossRefGoogle ScholarPubMed