Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-22T21:15:11.113Z Has data issue: false hasContentIssue false
  • English
  • Français

Gastro-jejunal digestion of soya-bean-milk protein in humans

Published online by Cambridge University Press:  09 March 2007

Agnes Baglieri ,
Sylvain Mahe ,
Semia Zidi ,
Jean-Francois Huneau ,
Francois Thuillier ,
Philippe Marteau  and
Daniel Tome
Agnes Baglieri
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Sylvain Mahe
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Semia Zidi
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Jean-Francois Huneau
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Francois Thuillier
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Philippe Marteau
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Daniel Tome
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In order to determine how soya-bean proteins are digested and metabolized in the human intestine before colonic bacterial fermentation and to estimate their true digestibility, the gastro-jejunal behaviour of soya-bean proteins in water and in two other forms (a concentrated soya-bean-protein solution (isolate) and a drink composed of crude soya-bean proteins (soymilk)) was studied in humans. Experiments were carried out in eight healthy volunteers using a double-lumen steady-state intestinal perfusion method with polyethyleneglycol (PEG) as a non-absorbable volume marker. Gastric emptying and N and electrolyte contents of the jejunal digesta were analysed. Gastric half-emptying time (min) of the liquid phase after water ingestion (12·59 (SE 0·12)) was shorter (P < 0.05) than those for soymilk (37·74 (SE 11·57)) and isolate (36·52 (SE 11·23)). Electrolytic balances showed that for all meals, Na+, Cl− and K+ were secreted when Ca2+ was efficiently absorbed from the jejunal lumen. Gastro-jejunal N absorption for isolate and soymilk were 63 and 49% respectively, and were not significantly different from one another; after water ingestion, endogenous N was estimated to be 21 mmol. An estimate of the exogenous: endogenous values for the effluents was obtained from the amino acid compositions of soymilk and effluents after water or soymilk ingestion, indicating that 70% of the total N was exogenous and 30% endogenous. Under these conditions the endogenous fraction represented 31 mmol after soymilk ingestion and the gastro-jejunal N balance indicated that 54% of the soymilk was absorbed. This finding indicates that the true gastro-jejunal digestibility of soya-bean proteins is similar to that of milk proteins.

Keywords

Soya-bean proteinDigestibilityHumans
Type
Digestion of soya bean milk in humans
Information
British Journal of Nutrition , Volume 72 , Issue 4 , October 1994 , pp. 519 - 532
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Beer, W. H., Murray, E., Oh, S. H., Pedersen, H. E., Wolfe, R. R. & Young, V. R. (1989). A long-term metabolic study to assess the nutritional value of and immunological tolerance to two soy-protein concentrates in adult humans. American Journal of Clinical Nutrition 50, 907–1007.CrossRefGoogle ScholarPubMed
Bodwell, C. E., Satterlee, L. D. & Hackler, L. R. (1980). Protein digestibility of the same protein preparations by humans and rat assays and by in vitro enzymic digestion methods. Journal of Nutrition 30, 269–283.Google Scholar
Brener, W., Hendrix, T. R. & McHugh, P. R. (1983). Regulation of the gastric emptying of glucose. Gastroenterology 85, 76–82.CrossRefGoogle ScholarPubMed
Bressani, R. & Elias, L. G. (1977). The problem of legume protein digestibility. In Nutritional Standards and Methods of Evaluation for Food Legume Breeders, pp. 61–72 [Billingsley, L. W., editor]. Ottawa: International Development Research Center.Google Scholar
Cheng, Y. J., Thompson, L. D. & Brittin, H. C. (1990). Sogurt, a yogurt-like soybean product: development and properties. Journal of Food Science 55, 1178–1179.CrossRefGoogle Scholar
Elashoff, J. D., Reedy, T. J. & Meyer, J. H. (1982). Analysis of gastric emptying data. Gastroenterology 83, 1306–1312.CrossRefGoogle ScholarPubMed
Emonts, P., Vidon, N., Bernier, J. J. & Rambaud, J. C. (1979). Etude sur 24 heures des flux liquidiens intestinaux chez I'homme normal par la technique de la perfusion lente d'un marqueur non absorbable. (24-hour intestinal water and electrolyte flow rates in normal man: assessment by the slow marker perfusion technique.) Gastroentirologie Clinique et Biologique 3, 139–146.Google Scholar
Erdman, J. W. & Fordyce, E. J. (1989). Soy products and the human diet. American Journal of Clinical Nutrition 49, 725–737.CrossRefGoogle ScholarPubMed
Fordtran, J. S. (1966). Marker perfusion techniques for measuring intestinal absorption in man. Gastroenterology 51, 1089–1093.Google Scholar
Fordtran, J. S. & Locklear, T. W. (1966). Ionic constituents and osmolality of gastric and small-intestinal fluids after eating. American Journal of Digestive Diseases 11, 503–521.CrossRefGoogle ScholarPubMed
Grant, G. (1989). Anti-nutritional effects of soyabean: A review. Progress in Food and Nutrition Science 13, 317–348.Google ScholarPubMed
Gorimar, T. S., Hernandez, H. A. & Polczynski, M. W. (1984). Rapid protein determination using pyrochemi-luminescence. American Clinical Products Review November, 46–49.Google Scholar
Guilloteau, P., Sauvant, D. & Patureau-Mirand, P. (1983). Methods of comparing amino acid composition of proteins: application to undigested proteins in the prenuminant calf. Annals of Nutrition and Metabolism 27, 457–469.CrossRefGoogle ScholarPubMed
Houghton, L. A., Mangnall, Y. F. & Read, N. W. (1990). Effect of incorporating fat into a liquid test meal on the relation between intragastric distribution and gastric emptying in human volunteers. Gut 31, 1226–1229.CrossRefGoogle ScholarPubMed
Hunt, J. N. & McDonald, I. (1954). The influence of volume on gastric emptying. Journal of Physiology 126, 459–474.CrossRefGoogle ScholarPubMed
Hyden, S. (1955). A turbidimetric method for the determination of higher polyethylene glycols in biological materials. Annals of the Royal Agriculture College of Sweden 21, 139–145.Google Scholar
Liener, I. E. (1981). Factors affecting the nutritional quality of soya products. Journal of the American Oil Chemists' Society 58, 406–415.CrossRefGoogle Scholar
Liener, I. E., Goodale, R. L., Deshmukh, A., Satterberg, T. L., Ward, G., DiPietro, C. M., Bankey, P. E. & Borner, J. W. (1988). Effect of a trypsin inhibitor from soybeans (Bowman-Birk) on the secretory activity of the human pancreas. Gastroenterology 94, 419–427.CrossRefGoogle ScholarPubMed
Mahe´, S., Huneau, J. F., Marteau, P., Thuillier, F. & Tome´, D. (1992). Gastro-ileal and electrolyte movements after bovine milk ingestion in humans. American Journal of Clinical Nutrition 56, 410–416.CrossRefGoogle Scholar
Mahe´, S., Roos, N., Benamouzig, R., Sick, H., Baglieri, A., Huneau, J. F. & Tome´, D. (1994). True exogenous and endogenous nitrogen fractions in the human jejunum after 15N-labeled casein ingestion in low amounts. Journal of Nutrition 124, 548–555.CrossRefGoogle Scholar
Malagelada, J. R., Longstreth, G. F., Summerskill, W. H. J. & Go, V. L. W. (1976). Measurement of gastric functions during digestion of ordinary solid meals in man. Gastroenterology 70, 203–210.CrossRefGoogle ScholarPubMed
Moberg, S. & Calberger, G. (1974). The effect of gastric emptying of test meals with various fat and osmolar concentrations. Scandinavian Journal of Gastroenterology 9, 29–32.CrossRefGoogle ScholarPubMed
Modigliani, R., Rambaud, J. C. & Bernier, J. J. (1973). The method of intraluminal perfusion of the human small intestine. 1. Principle and technique. Digestion 9, 176–192.CrossRefGoogle Scholar
Rackis, J. J. & Anderson, R. L. (1964). Isolation of four trypsin inhibitors by DEAE-cellulose chromatography. Biochemical and Biophysical Research Communications 15, 230–235.CrossRefGoogle ScholarPubMed
Rackis, J. J. & Gumbmann, M. R. (1981). Protease inhibitors: physiological properties and nutritional significance. In Antinutrients and Natural Toxicants in Foods, pp. 203–237 [Ory, R. L., editor]. Westport, CT: Food and Nutrition Press.Google Scholar
Roebuck, B. D. (1987). Trypsin inhibitors: potential concern for humans? Journal of Nutrifion 117, 398–400.CrossRefGoogle ScholarPubMed
Ruskone´, A., Cosnes, J., Vidon, N., Couzigou, P. & Bernier, J. J. (1980). Se´cre´tion et vidange gastrique apre´s differents repas homogeneises chez l' homme. (Gastric emptying and gastric secretion after variable homogenized meals in man.) Gastroenterologie Clinique et Biologique 4, 777–785.Google Scholar
SAS Institute Inc. (1990). SAS/STATTMUser's Guide, Release 6.03. Cary, NC: SAS Institute Inc.Google Scholar
Taylor, S. L., Lemanske, R. F., Bush, R. K. & Busse, W. W. (1987). Chemistry of food allergens. In Food Allergy [Chandra, R. K., editor]. St John's, Newfoundland: Nutrition Research Education Foundation.Google Scholar
Temler, R. S., Dormond, C. A., Simon, E., Morel, B. & Mettraux, C. (1984). Response of rat pancreatic proteases to dietary proteins, their hydrolysates and soybean trypsin inhibitor. Journal of Nutrition 114, 270–278.CrossRefGoogle ScholarPubMed
Torun, B., Viteri, F. E. & Young, V. R. (1981). Nutritional role of soya protein for humans. Journal of the American Oil Chemists' Society 58, 400–405.CrossRefGoogle Scholar
Vidon, N., Muschart, J. M., Cosnes, J., Ruskone, A. & Bernier, J. J. (1979). Etude critique de l'estimation de la vidange gastrique par la methode de perfusion duodenale d'une substance non absorbable a faible debit. (A critical study of gastric emptying by the dilution of a non-absorbable substance perfused in the duodenum at a slow flow rate.) Gastroenterologie Clinique et Biologique 3, 549–552.Google Scholar
Wang, M. F., Kishi, K., Takahashi, T., Komatsu, T., Ohnaka, M. & Inoue, G. (1983). Efficiency of utilization of soy protein isolate in Japanese young men. Journal of Nutritional Science and Vitaminology 29, 201–216.CrossRefGoogle ScholarPubMed
Wayler, A., Queiror, E., Scrimshaw, N. S., Steinke, F. H., Rand, W. M. & Young V. R. (1983). Nitrogen balance studies in young men to assess the protein quality of an isolated soy protein in relation to meat proteins. Journal of Nutrition 113, 2385–2391.CrossRefGoogle ScholarPubMed
Wolf, W. J. (1981). Progress and future needs for research in soya protein utilization and nutrition. Journal of the American Oil Chemists' Society 58, 467–473.CrossRefGoogle Scholar
Young, V. R., Scrimshaw, N. S., Torun, B. & Viteri, F. (1979). Soybean protein in human nutrition: an overview. Journal of the American Oil Chemists' Society 56, 110–120.CrossRefGoogle ScholarPubMed
Young, V. R., Wayler, A., Garza, C., Steinke, F. H., Murray, E., Rand, W. M. & Scrimshaw, N. S. (1984). A long term metabolic balance study in young men to assess the nutritional quality of an isolated soy protein and beef proteins. American Journal of Clinical Nutrition 39, 8–15.CrossRefGoogle ScholarPubMed