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β-Galactosidase production by Streptococcus thermophilus is higher in the small intestine than in the caecum of human-microbiota-associated mice after lactose supplementation

Published online by Cambridge University Press:  08 March 2007

Denis D.G. Mater ,
Sophie Drouault-Holowacz ,
Raish Oozeer ,
Philippe Langella ,
Jamila Anba  and
Gérard Corthier
Denis D.G. Mater
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Sophie Drouault-Holowacz
Affiliation:
Arilait Recherches, 42 rue de Châteaudun, 75009 Paris, France
Raish Oozeer
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Philippe Langella
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Jamila Anba
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Gérard Corthier*
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
*
*Corresponding author Dr Gérard Corthier, fax +33 134 652462, email [email protected]
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Abstract

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Transit kinetics and survival rates of a bacterial species from yoghurt (i.e. Streptococcus thermophilus strain FBI3) were examined in different digestive compartments of gnotoxenic and human-microbiota-associated mice. The production of the lactose-hydrolysing enzyme (i.e. β-galactosidase) was also investigated within the digestive tract, using a chromosomal reporter system based on luciferase genes from Photorhabdus luminescens under the control of the plac promoter. In both mice models, S. thermophilus cells transited within 2h from the stomach to the caecum–colon compartment of the digestive tract where they displayed a survival rate of nearly 100%. In gnotoxenic mice, luciferase activity was found to increase in the second half of the small intestine and in the caecum–colon compartment when lactose was added to the drinking water provided to the animals. In human-microbiota-associated mice drinking lactose, luciferase activity was similarly increased in the second half of the small intestine but was drastically reduced in the caecum–colon compartment. This feature could be ascribed to the presence of the resident human microbiota.

Keywords

Digestive tractHuman microbiotaLactose intoleranceStreptococcus thermophilusYoghurt
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 177 - 181
Copyright
Copyright © The Nutrition Society 2006

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