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Functional capacity of the residual lymphocytes from zinc-deficient adult mice

Published online by Cambridge University Press:  09 March 2007

Joan M. Cook-Mills
Affiliation:
Department of Biochemistry, Michigan State University, East Lansing, MI 48824, USA
Pamela J. Fraker
Affiliation:
Department of Biochemistry, Michigan State University, East Lansing, MI 48824, USA
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Abstract

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Zn deficiency has been shown to reduce host defence drastically. It was of interest to determine the capacity of the residual lymphocytes from Zn-deficient mice to proliferate and produce lymphokines in response to stimulation since there are many Zn-dependent metalloenzymes that might be altered by the deficiency. To address this question, young adult A/J mice were provided Zn-deficient or Zn-adequate diets or restricted amounts of a Zn-adequate diet for 30 d. Splenocytes from moderately or severely Zn- deficient adult A/J mice gave normal proliferative responses and generated adequate interleukin II (IL- 2) activity when stimulated with the mitogen Concanavalin A. However, splenocytes from deficient mice exhibited a higher degree of proliferation (about 150%) and production of IL-2 in response to foreign target cells compared with T-cells prepared from mice provided a Zn-adequate diet. B-cells from deficient mice stimulated in vivo with sheep erythrocytes produced fewer total numbers of plaque-forming cells (PFC) per spleen. Nevertheless, the proportion or number of PFC/106 viable splenocytes and the amounts of IgM and IgG antibody produced per PFC were equivalent to those of adequately-fed and restricted-fed controls. The previously described responses were not significantly affected by whether the level of Zn in the culture medium was adequate or limiting. Based on these tests it appeared that the residual splenic lymphocytes of Zn-deficient mice were able to carry out many fundamental immune processes.

Type
Mineral Metabolism
Copyright
Copyright © The Nutrition Society 1993

References

Allen, J. I., Korchik, W., Kay, N. E. & Mcclain, C. J. (1982). Zinc and T-lymphocyte function in hemodialysis patients. American Journal of Clinical Nutrition 36, 410415.CrossRefGoogle ScholarPubMed
American Institute of Nutrition (1977). Report of the AIN ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 13401348.CrossRefGoogle Scholar
Blomgren, H. & Svedmyr, E. (1971). In vitrostimulation of mouse thymus cells by phytohemaglutinin and allogeneic cells. Cellular Immunology 2, 285299.CrossRefGoogle Scholar
Carlomagno, M. A., Coghlan, L. G. & McMurray, D. N. (1986). Chronic zinc deficiency and listeriosis in rats: acquired cellular resistance and response to vaccination. Medicine, Microbiology and Immunology 175, 271280.CrossRefGoogle ScholarPubMed
Carlomagno, M. A. & McMurray, D. N. (1983). Chronic zinc deficiency in rats: its influence on some parameters of humoral and cell mediated immunity. Nutrition Research 3, 6978.CrossRefGoogle Scholar
Chesters, J. K., Petrie, L., Boyne, R. & Allen, G. (1988). Role of zinc in regulating ribosomal RNA synthesis vivo and in vitro. Journal of Trace Elements in Experimental Medicine 1, 117127.Google Scholar
Depasquale-Jardieu, P. & Fraker, P. J. (1980). Further characterization of the role of corticosterone in the loss of humoral immunity in zinc deficient A/J as determined by adrenalectomy. Journal of lmmunology 124, 26502655.Google ScholarPubMed
Dowd, P. S., Kelleher, J. & Guillou, P. J. (1986). T-lymphocyte subsets and interleukin II production in zinc deficient rats. British Journal of Nutrition 55, 5969.CrossRefGoogle ScholarPubMed
Fauci, A. S., Dale, D. C. & Balow, J. E. (1976). Glucocorticosteroid therapy: mechanisms of action and clinical consideration. Annals of Internal Medicine 84, 315339.CrossRefGoogle Scholar
Fernandes, G., Nair, M., Onoe, K., Tanaka, T., Floyd, R. & Good, R. (1979). Impairment of cell mediated immunity function by dietary zinc deficiency in mice. Proceedings of the National Academy of Sciences, USA 76, 457461.CrossRefGoogle ScholarPubMed
Fraker, P. J., Gershwin, M. E., Good, R. A. & Prasad, A. (1986). Interrelationships between zinc and immune function. Federution Proceedings 45, 14741479.Google ScholarPubMed
Fraker, P. J., Haas, S. M. & Luecke, R. W. (1977). Effect of zinc deficiency on the immune response of the young adult A/J mouse. Journal of Nutrition 107, 18891895.CrossRefGoogle ScholarPubMed
Fraker, P. J., Jardieu, P. & Cook, J. (1987). Zinc deficiency and immune function. Archives ofDermatology 123, 16991701CrossRefGoogle ScholarPubMed
Fraker, P. J. & Speck, J. C. Jr (1978). Protein and cell membrane iodinations with a sparingly soluble chloramide, 1,3,4,6-tetrachloro-3a, 6a-diphenyl-glycoluril. Biochemical and Biophysical Research Communications 80, 849857.CrossRefGoogle Scholar
Fraker, P. J., Zwicki, C. M. & Luecke, R. W. (1982). Delayed type hypersensitivity in zinc deficient adult mice: impairment and restoration of responsivity to dinitrofluorobenzene. Journal of Nutrition 112, 309313.CrossRefGoogle ScholarPubMed
Frost, P., Chen, J. C., Rabbani, I., Smith, J. & Prasad, A. (1977). The effect of zinc deficiency on the immune response. In Zinc Metabolism: Current Aspects in Health and Disease, pp. 143150 [Brewer, G. J. and Prasad, A., editors]. New York: A. R. Liss.Google Scholar
Gillis, S., Fern, M. M., Ou, W. & Smith, K. S. (1978). T-cell growth factor: parameters of production and a quantitative microassay for activity. Journal of Immunology 120, 20272031.CrossRefGoogle Scholar
Gross, R., Osdin, N., Fong, L. & Newberne, P. (1979). Depressed immunological function in zinc-deprived rats measured by mitogen response of spleen, thymus and peripheral blood. American Journnl of Clinical Nurrition 32, 12601265.CrossRefGoogle ScholarPubMed
King, L. & Fraker, P. (1991). Flow cytometric analysis of the phenotypic distribution of splenic lymphocytes in zinc-deficient adult mice. Journal of Nutrition 121, 14331438.CrossRefGoogle ScholarPubMed
Kramer, T. (1984). Re-evaluation of zinc deficiency on Concanavalin A induced rat spleen lymphocyte proliferation. Journal of Nutrition 114, 953963.CrossRefGoogle Scholar
Lee, K. C. (1977). Cortisone as a probe for cell interactions in the generation of cytotoxic T-cells. Journal of Immunology 119, 18361845.CrossRefGoogle ScholarPubMed
Luecke, R. W., Simonel, C. & Fraker, P. J. (1978). The effect of restricted dietary intake on the antibody mediated response of the zinc deficient A/J mouse. Journal of Nutrition 108, 881887.CrossRefGoogle ScholarPubMed
Mishell, B. B. & Shiigi, S. M. (1980). Cell proliferation. In Selected Methods in Cellular Immunology, pp. 153172. San Francisco: W. H. Freeman Co.Google Scholar
Pekarek, R. S., Sandstead, H. H., Jacob, R. A. & Barcome, D. F. (1979). Abnormal cellular immune responses during acquired zinc deficiency. American Journal of Clinical Nutrition 32, 14661471.CrossRefGoogle ScholarPubMed
Prasad, A. (1984). Discovery and importance of zinc in human nutrition. Federation Proceedings 43, 28292834.Google ScholarPubMed
Wirth, J. J., Fraker, P. J. & Kierszenbaum, F. (1984). Changes in the levels of marker expression by mononuclear phagocytes in zinc deficient mice. Journal of Nutrition 114, 18261833.CrossRefGoogle ScholarPubMed