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Fermentation of non-starch polysaccharides in mixed diets and single fibre sources: comparative studies in human subjects and in vitro

Published online by Cambridge University Press:  09 March 2007

Elisabeth Wisker ,
Martina Daniel ,
Gerhard Rave  and
Walter Feldheim
Elisabeth Wisker*
Affiliation:
Christian Albrechts-University of Kiel, Institute of Human Nutrition and Food Science, Düsternbrooker Weg 17, D-24105 Kiel, Germany
Martina Daniel
Affiliation:
Christian Albrechts-University of Kiel, Institute of Human Nutrition and Food Science, Düsternbrooker Weg 17, D-24105 Kiel, Germany
Gerhard Rave
Affiliation:
Christian Albrechts-University of Kiel, Variationsstatistik, Olshausenstraβe 40, D-24098 Kiel, Germany
Walter Feldheim
Affiliation:
Christian Albrechts-University of Kiel, Institute of Human Nutrition and Food Science, Düsternbrooker Weg 17, D-24105 Kiel, Germany
*
*Corresponding author: Dr Elisabeth Wisker, fax +49 431 880 1528, email [email protected]
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Abstract

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The present study investigated whether the extent of fermentation of NSP in human subjects could be predicted by an in vitro batch system. Fibre sources studied were five mixed diets containing different amounts and types of fibre and three single fibre sources (citrus fibre concentrate, coarse and fine wholemeal rye bread). Fermentation in human subjects was determined in balance experiments in women who were also donors of the faecal inocula. In vitro fermentations were performed with fibre residues prepared from duplicates of the fibre-containing foods consumed during the balance trials. Fermentation of total NSP in vivo was between 65.8 and 88.6% for the mixed diets and 54.4, 58.0 and 96.9 % for the coarse and fine wholemeal rye breads and the citrus fibre concentrate respectively. For the mixed diets and the citrus fibre concentrate, mean differences between the extent of NSP degradation after 24 h in vitro incubation and that in vivo were between −0.7 and 5.0 %. Differences were significant for one diet (P < 0.05). For the wholemeal rye breads, the fermentation in vitro exceeded that in vivo significantly, but the magnitude of the difference in each case was small and without physiological importance. Particle size of breads had no influence on the extent of NSP degradation. These results indicate that the in vitro batch system used could provide quantitative data on the fermentation in vivo of NSP in mixed diets and some single fibre sources. An in vitro incubation time of 24 h was sufficient to mimic the NSP degradation in vivo.

Keywords

FermentationNon-starch polysaccharidesIn vitro batch system
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 3 , September 1998 , pp. 253 - 261
Copyright
Copyright © The Nutrition Society 1998

References

Adiotomre, J, Eastwood, MA, Edwards, CA & Brydon, WG (1990) Dietary fiber: in vitro methods that anticipate nutrition and metabolic activity in humans. American Journal of Clinical Nutrition 52, 128–134.CrossRefGoogle ScholarPubMed
Bach, Knudsen KE, Wisker, E, Daniel, M, Feldheim, W & Eggum, BO (1994) Digestibility of energy, protein, fat and non-starch polysaccharides in mixed diets: comparative studies between man and the rat. British Journal of Nutrition 71, 471–487.Google Scholar
Barry, J-L, Hoebler, C, Macfarlane, GT, Macfarlane, S, Mathers, JC, Reed, KA, Mortensen, PB, Nordgaard, I, Rowland, IR & Rumney, CJ (1995) Estimation of the fermentability of dietary fibre in vitro: a European interlaboratory study. British Journal of Nutrition 74, 303–322.CrossRefGoogle ScholarPubMed
Bingham, S (1990) Mechanisms and epidemiological evidence relating dietary fibre (non-starch polysaccharides) and starch to protection against large bowel cancer. Proceedings of the Nutrition Society 49, 153–171.CrossRefGoogle ScholarPubMed
Björck, I, Nyman, M, Pedersen, B, Siljeström, M, Asp, N-G & Eggum, BO (1986) On the digestibility of starch in wheat bread - Studies in vitro and in vivo. Journal of Cereal Science 4, 1–11.CrossRefGoogle Scholar
Bourquin, LD, Titgemeyer, EC, Garleb, KA & Fahey, GC (1992) Short-chain fatty acid production and fiber degradation by human colonic bacteria: effects of substrate and cell wall fractionation procedures. Journal of Nutrition 122, 1508–1520.Google Scholar
Bourquin, LD, Titgemeyer, EC & Fahey, GC (1993) Vegetable fiber fermentation by human fecal bacteria: cell wall polysaccharide disappearance and short-chain fatty acid production during in vitro fermentation and water-holding capacity of unfermented residues. Journal of Nutrition 123, 860–869.CrossRefGoogle ScholarPubMed
Daniel, M, Wisker, E, Rave, G & Feldheim, W (1997) Fermentation in human subjects of nonstarch polysaccharides in mixed diets, but not in a barley fiber concentrate, could be predicted by in vitro fermentation using human fecal inocula. Journal of Nutrition 127, 1981–1988.CrossRefGoogle ScholarPubMed
Edwards, CA, Bowen, J & Eastwood, M (1990) The effect of isolated complex carbohydrate on caecal and faecal short chain fatty acids and stool output in the rat. In Dietary Fibre. Chemical and Biological Aspects, pp. 273–276 [Southgate, DAT, Waldron, K, Johnson, IT and GR, Fenwick, editors]. Cambridge, Royal Society of Chemistry.Google Scholar
Edwards, CA & Rowland, IR (1992) Bacterial fermentation in the colon and its measurement. In Dietary Fibre–A Component of Food. Nutritional Function in Health and Disease, pp. 119–136 [Schweizer, TF and Edwards, CA, editors]. London: Springer.CrossRefGoogle Scholar
Englyst, HN, Wiggins, HS & Cummings, JH (1982) Determination of non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107, 307–318.CrossRefGoogle ScholarPubMed
Goering, HK & Van, Soest PJ (1970) Forage Fiber Analyses. Agriculture Handbook no. 379. Washington, DC: United States Department of Agriculture, U.S. Government Printing Office.Google Scholar
Goodlad, JS & Mathers, JC (1991) Digestion by pigs of non-starch polysaccharides in wheat and raw peas (Pisum sativum) fed in mixed diets. British Journal of Nutrition 65, 259–270.Google Scholar
Guillon, F, Barry, J-L & Thibault, J-F (1992) Effect of autoclaving sugar-beet fibre on its physico-chemical properties and its in-vitro degradation by human faecal bacteria. Journal of the Science of Food and Agriculture 60, 69–79.CrossRefGoogle Scholar
Guillon, F, Cloutour, F & Barry, J-L (1996) Dietary fibre: relations between intrinsic characteristics and fermentation pattern. In Dietary Fibre and Fermentation in the Human Colon. Proceedings of the COST Action 92 Workshop, pp. 110–115 [Mälkki, Y and Cummings, JH, editors]. Brussels: Office for Official Publications of the European Communities.Google Scholar
Hoskins, LC & Boulding, ET (1981) Mucin degradation in human colonic ecosystem. Evidence for the existence and role of bacterial subpopulations producing glycosidases as extracellular enzymes. Journal of Clinical Investigation 67, 163–172.CrossRefGoogle ScholarPubMed
Key, FB & Mathers, JC (1993) Complex carbohydrate digestion and large bowel fermentation in rats given wholemeal bread and haricot beans (Phaseolus vulgaris) fed in mixed diets. British Journal of Nutrition 69, 497–509.CrossRefGoogle ScholarPubMed
Lebet, V, Jörger, F, Arrigoni, E, Wenk, C & Amadò, R (1996) Influence of a simulated digestion procedure on in vitro colonic fermentation patterns. In Dietary Fibre and Fermentation in the Human Colon. Proceedings of the COST Action 92 Workshop, pp. 101–109 [Mälkki, Y and Cummings, JH, editors]. Brussels: Office for Official Publications of the European Communities.Google Scholar
Longland, AC & Low, AG (1988) Digestion of diets containing molassed or plain sugar-beet pulp by growing pigs. Animal Feed Science and Technology 23, 67–78.CrossRefGoogle Scholar
McBurney, MI & Thompson, LU (1987) Effect of human faecal inoculum on in vitro fermentation variables. British Journal of Nutrition 58, 233–243.CrossRefGoogle ScholarPubMed
McBurney, MI & Thompson, LU (1989) Effect of human faecal donor on in vitro fermentation variables. Scandinavian Journal of Gastroenterology 24, 359–367.Google Scholar
McNeil, NI (1984) The contribution of the large intestine to energy supplies in humans. American Journal of Clinical Nutrition 39, 338–342.Google Scholar
Marlett, JA, Slavin, MS & Brauer, PM (1981) Comparison of dye and pellet gastrointestinal transit time during controlled diets differing in protein and fiber levels. Digestive Diseases and Sciences 26, 208–213.CrossRefGoogle ScholarPubMed
Metcalf, AM, Phillips, SF, Zinsmeister, AR, MacCarthy, RL, Beart, RW & Wolff, BG (1987) Simplified assessment of segmental colonic transit. Gastroenterology 92, 40–47.CrossRefGoogle ScholarPubMed
Monsma, DJ & Marlett, JA (1996) Fermentation of carbohydrate in rat ileal excreta is enhanced with cecal inocula compared with fecal inocula. Journal of Nutrition 126, 554–563.CrossRefGoogle ScholarPubMed
Prosky, L, Asp, N-G, Furda, I, DeVries, JH, Schweizer, TF & Harland, B (1985) Determination of total dietary fiber in foods and food products: collaborative study. Journal of the Association of Official Analytical Chemists 68, 677–679.Google ScholarPubMed
Roediger, WEW (1982) Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 83, 424–429.Google Scholar
Ruppin, H, Bar-Meir, S, Soergel, KH, Wood, CM & Schmitt, MG Jr (1980) Absorption of short-chain fatty acids by the colon. Gastroenterology 78, 1500–1507.CrossRefGoogle ScholarPubMed
Southgate, DAT & Durnin, JVGA (1970) Calorie conversion factors: an experimental reassessment of the factors used in the calculation of the energy value of human diets. British Journal of Nutrition 24, 517–535.CrossRefGoogle ScholarPubMed
Stephen, AM & Cummings, JH (1980) Mechanism of action of dietary fibre in the human colon. Nature 284, 283–284.CrossRefGoogle ScholarPubMed
Theander, O & Westerlund, EA (1986) Studies on dietary fiber. 3. Improved procedure for analysis of dietary fiber. Journal of Agricultural and Food Chemistry 34, 330–336.CrossRefGoogle Scholar
Titgemeyer, EC, Bourquin, LD, Fahey, GC & Garleb, KA (1991) Fermentability of various fiber sources by human fecal bacteria in vitro. American Journal of Clinical Nutrition 53, 1418–1424.CrossRefGoogle ScholarPubMed
Van Soest, PJ, Jeraci, J, Foose, T, Wrick, K & Ehle, F (1982) Comparative fermentation of fibre in man and other animals. In Fibre in Human and Animal Nutrition, pp. 75–80 [Wallace, G and Bell, L, editors]. Palmerston North: The Royal Society of New Zealand.Google Scholar
Wisker, E, Bach Knudsen, KE, Daniel, M, Eggum, BO & Feldheim, W (1997) Energy values of non-starch polysaccharides: comparative studies in humans and rats. Journal of Nutrition 127, 108–116.Google Scholar
Wisker, E, Daniel, M & Feldheim, W (1996) Particle size of wholemeal rye bread does not affect the digestibility of macro-nutrients and non-starch polysaccharides and the energy value of dietary fibre in humans. Journal of the Science of Food and Agriculture 70, 327–333.3.0.CO;2-0>CrossRefGoogle Scholar
Wyman, JB, Heaton, KW, Manning, AP & Wicks, ACB (1978) Variability of colonic function in healthy subjects. Gut 19, 146–150.CrossRefGoogle ScholarPubMed