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Excretion of benzoic acid derivatives in urine of sheep given intraruminal infusions of 3-phenylpropionic and cyclohexanecarboxylic acids

Published online by Cambridge University Press:  24 July 2007

J. H. Pagella ,
X. B. Chen ,
N. A. Macleod ,
E. R. Ørskov  and
P. J. S. Dewey
J. H. Pagella
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
X. B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
N. A. Macleod
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
P. J. S. Dewey
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
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Abstract

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The quantitative relationship between the urinary excretion of benzoic acid (BA)and the uptake of 3-phenylpropionic (PPA) and cyclohexanecarboxylic (CHCA) acids was assessed.PPA and CHCA are produced in the rumen by microbial fermentation of lignocellulosic feeds and metabolized, after absorption, to BA which is excreted in the urine mainly as its glycine conjugate hippuric acid (HA). Four sheep nourished by intragastric infusions of all nutrients weregiven continuous ruminal infusions of PPA (8,16 or 24 mmol/d) either alone or with CHCA (8 or 16 mmol/d) in a factorial experiment. The treatments were allocated to ten consecutive 6 d periods, with a control being repeated at periods 1, 5 and 10. PPA and CHCA ruminal absorption rates, estimated using the liquid-phase marker Cr-EDTA, were 0·78 (SD 0·29)/h and 0·88 (SD 0·28)/h respectively. For the control, HA excretion was only 0·22 (SD 0·33) mmol/d and free BA was absent. For the other treatments, both HA and free BA were present and HA accounted for 0·85 (SD 0·05) of total BA. The urinary excretion of total BA showed a significant linear correlation (r = 0·997, P<0·001) with the amounts of PPA and CHCA infused. The urinary recovery of infused PPA and CHCA as total BA was 0·79 (SE 0·01). Faecal excretion of BA and its precursors was negligible. Results of this study show that urinary total BA is a potential estimator of the absorption of PPA + CHCA produced in the rumen

Keywords

Benzoic acidHippuric acid3-Phenylpropionic acidCyclohexanecarboxylic acidSheep
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 4 , April 1997 , pp. 577 - 592
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Technical Review by an Agricultural Research Council Working Party. Farnham Royal: Commonwealth Agricultural Bureau.Google Scholar
Binnerts, W. T., van' Klooster, A. T. & Frens, A. M. (1968). Soluble chromium indicator measured by atomic absorption in digestion experiments. Veterinary Record 82, 470.Google Scholar
Brewster, D., Jones, R. R. & Parke, D. V. (1977). The metabolism of cyclohexanecarboxylate in the rat. Biochemical Journal 164, 595–600.CrossRefGoogle Scholar
Caldwell, J. (1989). Xenobiotic metabolism: an introduction. In Intermediary Xenobiotic Metabolism in Animals: Methodology, Mechanisms and Significance, pp. 3–12 [Hutson, D. H., Caldwell, J. and Paulson, G. D. editors]. London: Taylor and Francis.Google Scholar
Chen, X. B., Mejía, A. T., Pagella, J. H., Kyle, D. J. & Ørskov, E. R. (1993). Urinary excretion of purine derivatives and benzoic acid as an indication of microbial protein supply and cellulosic feed intake in sheep. In Proceedings of the 22 Rumen Function Conference, vol. 22, p. 15. Chicago, IL: Agricultural Research Service and US Department of Agriculture.Google Scholar
Chen, X. B., Pagella, J. H., Bakker, M. L. & Parra, O. (1996). Determination of aromatic metabolites in ruminant urine by high performance liquid chromatography. Journal of Chromatography B 682, 201–208.CrossRefGoogle ScholarPubMed
Czerkawski, J. W. (1986). Introduction to Rumen Studies. Oxford: Pergamon Press.Google Scholar
Genstat 5 Committee (1993). Genstat 5 Release Reference Manual. Oxford: Oxford University Press.Google Scholar
Giráldez, F. J., Zorita, E. & Peláez, R. (1993). Urinary hippuric acid excretion as related to changes in cell wall digestibility due to variation in degradable protein supply. In Animal Production in Developing Countries, Occasional Publication no. 16, pp. 192–193 [Gill, M., Owen, E., Pollot, G. E. and Lawrence, T. L. J. editors]. Midlothian: British Society of Animal Production.Google Scholar
Goodall, E. D. & Kay, R. N. B. (1973). The use of the chromium complex of ethylenediaminetetra-acetic acid for studies of digestion in sheep. Proceedings of the Nutrition Society 32, 22A–23A.Google ScholarPubMed
Grümer, H. D. (1961). Formation of hippuric acid from phenylalanine labelled with carbon-14 in phenylketonuric subjects. Nature 189, 63–64.CrossRefGoogle Scholar
Hansen, R. P. & Gerson, T. (1967). The isolation of 11-cyclohexylundecanoic acid from sheep perinephric fat. Journal of the Science of Food and Agriculture 18, 225–227.CrossRefGoogle ScholarPubMed
Hedrick, H. B., Bailey, M. E., Dupuy, H. P. & Legendre, M. G. (1980). Relationship between volatile compounds in fat from forage and grain-fed beef and sensory characteristics of steaks and roasts. In Proceedings of the 26 European Meeting of Meat Research Workers Volume of Abstracts, p. 307. Colorado Springs: American Meat Science Association.Google Scholar
Hirom, P. C., Millburn, P., Smith, R. L. & William, R. T. (1972). Species variation in the threshold molecularweight factor for the biliary excretion of organic anions. Biochemical Journal 129, 1071–1077.CrossRefGoogle ScholarPubMed
Hutt, J. H. & Caldwell, J. (1990). Amino acid conjugation. In Conjugation Reactions in Drug Metabolism: An Integrated Approach, pp. 273–305 [Mulder, G. J. editor]. London: Taylor and Francis.Google Scholar
Jenkins, W. L., Davis, L. E. & Boulos, B. M. (1975). Transfer of drugs across the ruminal wall in goats. American Journal of Veterinary Research 36, 1771–1776.Google ScholarPubMed
Kreula, M., Raumaramaa, A. & Tengengren, M. (1970). On the metabolism of benzoic acid by cows on purified protein-free and low-protein feed. Journal of the Scientific Agricultural Society of Finland 50, 177–181.Google Scholar
Larick, D. K. & Turner, B. E. (1990). Headspace volatile and sensory characteristics of ground beef from foragefed and grain-fed heifers. Journal of Food Science 54, 649–654.CrossRefGoogle Scholar
MacLeod, N. A., Comgal, W., Stirton, R. A. & Ørskov, E. R. (1982). Intragastric infusion of nutrients in cattle. British Journal of Nutrition 47, 547–552.CrossRefGoogle ScholarPubMed
Martin, A. K. (1966). Metabolism of benzoic acid by sheep. Journal of the Science of Food and Agriculture 17, 496–500.CrossRefGoogle ScholarPubMed
Martin, A. K. (1969). Urinary excretion of aromatic acids by sheep given diets containing different amounts of protein and roughage. British Journal of Nutrition 23, 389–399.CrossRefGoogle ScholarPubMed
Martin, A. K. (1973). Urinary aromatic excretion by fed and fasted sheep in relation to protein metabolism in the rumen. British Journal of Nutrition 30, 251–267.CrossRefGoogle ScholarPubMed
Martin, A. K. (1978). The metabolism of aromatic compounds in ruminants. In The Hannah Research Institute, 1928–1978, Essays of the Scientific Work of the Institute, pp. 148–163 [Moore, J. H. and Rook, J. A. F. editors]. Ayr: Hannah Research Institute.Google Scholar
Martin, A. K. (1982 a). The origin of urinary aromatic compounds excreted by ruminants 1. The metabolism of quinic, cyclohexanecarboxylic and non-phenolic aromatic acids to benzoic acid. British Journal of Nutrition 47, 139–154.CrossRefGoogle Scholar
Martin, A. K. (1982 b). The origin of urinary aromatic compounds excreted by ruminants 2. The metabolism of phenolic cinnamic acids to benzoic acid. British Jouml of Nutrition 47, 155–164.CrossRefGoogle Scholar
Ørskov, E. R., Grub, D. A., Wenham, G. & Conigall, W. (1979). The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acids and protein. British Journal of Nutrition 41, 553–558.CrossRefGoogle Scholar
Ringer, A. I. (1911). On the maximum production of hippuric acid in animals with consideration of the origin of glycocoll in the animal body. Journal of Biological Chemistry 10, 327–338.CrossRefGoogle Scholar
Ross, G. J. S. (1987). Maximum Likelihood Program User Manual. Oxford: The Numerical Algorithms Group.Google Scholar
Scheline, R. R. (1978). Mammalian Metabolism of Plant Xenabiotics. London: Academic Press.Google Scholar
Schogt, J. C. M. & Haverkamp Begemann, P. (1965). Isolation of 11-cyclohexylundecanoic acid from butter. Journal of Lipid Research 6, 466–470.CrossRefGoogle ScholarPubMed
Scott, T. W., Ward, P. F. V. & Dawson, R. M. C. (1964). The formation and metabolism of phenyl-substituted fatty acids in the ruminant. Biochemical Journal 90, 12–24.CrossRefGoogle ScholarPubMed
Weekes, T. E. C. (1976). Effects of infusion of volatile fatty acids into the rumen on insulin release in sheep. Journal of Physiology 254, 8OP–81P.Google ScholarPubMed