Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-25T04:40:25.687Z Has data issue: false hasContentIssue false
  • English
  • Français

Energy balance and liver respiratory activity in rats fed on an energy-dense diet

Published online by Cambridge University Press:  07 September 2009

Susanna Iossa ,
Maria P. Mollica ,
Lillà Lionetti ,
Antonio Barletta  and
Giovanna Liverini
Susanna Iossa
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Maria P. Mollica
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Lillà Lionetti
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Antonio Barletta
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Giovanna Liverini
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In the present study energy balance and liver respiratory activity were studied in rats fed on either a control diet or an energy-dense diet. Liver respiration was assessed both without added substrates and after the addition of hexanoate, glycerol, or sorbitol. The effect of ouabain on hexanoate-supported respiration was also determined. Metabolizable energy intake and energy expenditure increased in rats fed on an energy dense diet, but body-weight gain, as well as lipid and protein content, remained unchanged. When net energy expenditure, obtained excluding the total cost of storage, was expressed as a percentage of metabolizable energy, significant differences were found between the two groups of rats. This finding supports the presence of regulatory mechanisms in rats fed on an energy-dense diet, which are useful to counteract development of obesity. In addition, a significant increase in liver respiratory activity was found in rats fed on an energy-dense diet, both in the basal state and in that stimulated by added substrates. Na/K-pump-dependent O2 consumption also increased in rats fed on an energy-dense diet. The results indicate that a greater production of metabolic heat by the liver can contribute to the increased energy expenditure found in rats fed on an energy-dense diet.

Keywords

Energy balanceLiver respirationFat intake
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 1 , January 1997 , pp. 99 - 105
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Allard, M. & LeBlanc, J. (1988). Effects of cold acclimation, cold exposure and palatability on postprandial thermogenesis in rats. International Journal of Obesity 12, 169–178.Google ScholarPubMed
American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.Google Scholar
American Institute of Nutrition (1980). Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.Google Scholar
Barr, H. G. & McCracken, K. J. (1984). High efficiency of energy utilization in “cafeteria” and force-fed rats kept at 29°C. British Journal of Nutrition 51, 379–387.Google Scholar
Berry, M. N., Clark, D. G., Grivell, A. R. & Wallace, P. G. (1985). The contribution of hepatic metabolism to diet-induced thermogenesis. Metabolism 34, 141–147.CrossRefGoogle ScholarPubMed
Berry, M. N., Kun, E. & Werner, H. V. (1973). Regulatory role of reducing-equivalent transfer from substrate to oxygen in the hepatic metabolism of glycerol and sorbitol. European Journal of Biochemistry 33, 407–417.CrossRefGoogle ScholarPubMed
Dulloo, A. G. & Girardier, L. (1993). 24 hour energy expenditure several months after weight loss in the underfed rat: evidence for a chronic increase in whole body metabolic efficiency. International Journal of Obesity 11, 115–123.Google Scholar
Garrow, J. S. (1986). Chronic effects of over and under-nutrition on thermogenesis. International Journal of Vitamin and Nutrition Research 56, 201–204.Google ScholarPubMed
Griggio, M. A., Luz, J. & Carvalho, S. M. T. (1992). The effect of fasting and refeeding on oxygen consumption by rats. Brazilian Journal of Medicine and Biological Research 25, 205–208.Google ScholarPubMed
Hill, J. O., Latiff, A. & DiGirolamo, M. (1988). Effects of variable caloric restriction on utilization of ingested energy in rats. American Journal of Physiology 248, 549–559.Google Scholar
Iossa, S., Liverini, G. & Barletta, A. (1991). Physiological changes due to cold adaptation in rat liver. Cellular Physiology and Biochemistry 1, 226–236.CrossRefGoogle Scholar
Iossa, S., Mollica, M. P., Lionetti, L., Barletta, A. & Liverini, G. (1995). Hepatic mitochondrial respiration and transport of reducing equivalents in rats fed an energy dense diet. International Journal of Obesity 19, 539–543.Google Scholar
LeBlanc, J., Lupien, D., Diamond, P., Macari, M. & Richard, D. (1986). Thermogenesis in response to various intakes of palatable food. Canadian Journal of Physiology and Pharmacology 64, 976–982.CrossRefGoogle ScholarPubMed
Liverini, G., Iossa, S. & Barletta, A. (1994). Hepatic mitochondrial respiratory capacity in hyperphagic rats. Nutrition Research 11, 1671–1682.Google Scholar
Liverini, G., Iossa, S., Lionetti, L., Mollica, M. P. & Barletta, A. (1995). Sympathetically-mediated thermogenic response to food in rats. International Journal of Obesity 19, 87–91.Google Scholar
Ma, S. W. Y. & Foster, D. O. (1986). Starvation-induced changes in metabolic rate, blood flow, and regional energy expenditure in rats. Canadian Journal of Physiology and Pharmacology 64, 1252–1258.Google Scholar
Ma, S. W. Y., Nadeau, B. A. & Foster, D. O. (1987). Evidence for liver as the major site of diet-induced thermogenesis of rats fed a “cafeteria” diet. Canadian Journal of Physiology and Pharmacology 65, 1802–1804.CrossRefGoogle ScholarPubMed
Naim, M., Brand, J. G., Kare, M. R. & Carpenter, R. G. (1985). Energy intake, weight gain, and fat deposition in rats fed nutritionally controlled diets in a multichoice (“cafeteria”) design. Journal of Nutrition 115, 1447–1458.CrossRefGoogle Scholar
Nobes, C. D., Hay, W. W. & Brand, M. D. (1990). The mechanism of stimulation of respiration by fatty acids in isolated hepatocytes. Journal of Biological Chemistry 265, 12910–12915.CrossRefGoogle ScholarPubMed
Pullar, J. D. & Webster, J. F. (1977). The energy cost of fat and protein deposition in the rat. British Journal of Nutrition 37, 355–363.Google Scholar
Rothwell, N. J. & Stock, M. J. (1982). Energy expenditure of “cafeteria-fed” rats determined from measurements of energy balance and indirect calorimetry. Journal of Physiology 382, 371–377.Google Scholar
Rothwell, N. J. & Stock, M. J. (1986), Brown adipose tissue and diet induced thermogenesis. In Brown Adipose Tissue, pp. 269–283 [Trayhurn, P. and Nicholls, D. G., editors]. London: Edward Arnold.Google Scholar
Rothwell, N. J., Stock, M. J. & Tyzbir, R. S. (1982). Energy balance and mitochondrial function in liver and brown fat of rats fed “cafeteria” diet of varying protein content. Journal of Nutrition 112, 1663–1672.CrossRefGoogle Scholar
Schwenke, W. D., Soboll, S., Seitz, H. G. & Seis, H. (1981). Mitochondrial and cytosolic ATP/ADP ratios in rat liver in vivo. Biochemical Journal 200, 405–408.CrossRefGoogle ScholarPubMed
Seglen, P. O. (1974). Preparation of isolated rat liver cells. Methods in Cell Biology 8, 29–83.Google Scholar
Sherratt, H. S. A. & Spurway, T. D. (1994). Regulation of fatty acid oxidation in cells. Biochemical Society Transactions 22, 423–427.CrossRefGoogle ScholarPubMed
Soboll, S. & Stucki, J. (1985). Regulation of the degree of coupling of oxidative phosphorylation in intact rat liver. Biochimica et Biophysica Acta 807, 245–254.Google Scholar
Tyzbir, R. S., Kunin, A. S., Sims, N. M. & Danforth, E. (1981). Influence of diet composition on serum triiodothyronine (T3) concentration, hepatic mitochondrial metabolism and shuttle system activity in rats. Journal of Nutrition 111, 252–259.Google Scholar