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Energy balance and liver respiratory activity in rats fed on an energy-dense diet

Published online by Cambridge University Press:  07 September 2009

Susanna Iossa ,
Maria P. Mollica ,
Lillà Lionetti ,
Antonio Barletta  and
Giovanna Liverini
Susanna Iossa
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Maria P. Mollica
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Lillà Lionetti
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Antonio Barletta
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
Giovanna Liverini
Affiliation:
Department of General and Environmental Physiology, University of Naples, “FEDERICO II”, Naples, Italy
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Abstract

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In the present study energy balance and liver respiratory activity were studied in rats fed on either a control diet or an energy-dense diet. Liver respiration was assessed both without added substrates and after the addition of hexanoate, glycerol, or sorbitol. The effect of ouabain on hexanoate-supported respiration was also determined. Metabolizable energy intake and energy expenditure increased in rats fed on an energy dense diet, but body-weight gain, as well as lipid and protein content, remained unchanged. When net energy expenditure, obtained excluding the total cost of storage, was expressed as a percentage of metabolizable energy, significant differences were found between the two groups of rats. This finding supports the presence of regulatory mechanisms in rats fed on an energy-dense diet, which are useful to counteract development of obesity. In addition, a significant increase in liver respiratory activity was found in rats fed on an energy-dense diet, both in the basal state and in that stimulated by added substrates. Na/K-pump-dependent O2 consumption also increased in rats fed on an energy-dense diet. The results indicate that a greater production of metabolic heat by the liver can contribute to the increased energy expenditure found in rats fed on an energy-dense diet.

Keywords

Energy balanceLiver respirationFat intake
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 1 , January 1997 , pp. 99 - 105
Copyright
Copyright © The Nutrition Society 1997

References

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