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Endogenous purine and pyrimidine derivative excretion in pregnant sows

Published online by Cambridge University Press:  09 March 2007

S. M. Martin Orue ,
J. Balcells ,
J. A. Guada  and
C. Castrillo
S. M. Martin Orue
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
J. Balcells
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
J. A. Guada
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
C. Castrillo
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
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Abstract

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The present experiment was carried out to study the endogenous losses of purine and pyrimidine derivatives from pregnant sows. Three pregnant and three non-pregnant Large White × Landrace sows were fed on a purine-free diet composed of starch, glucose, sucrose and vegetable oil, with casein as the protein source. The experiment began, for the six animals, after diagnosis of pregnancy and was divided into six 12 d periods. Urine was collected during the first 3 d of each experimental period by means of a urethral catheter for determination of allantoin, uric acid, xanthine, hypoxanthine and pseudouridine concentrations. In the absence of dietary nucleic acids (N A), allantoin and, as a consequence, excretion of total purine derivatives (PD) decreased significantly to a constant value (128·3 (se 7·07) μmol/kg metabolic live weight (W0·7) per d), an amount assumed to represent endogenous excretion. Excretion of uric acid (38·7 (SE 2·15) μmol/kg W0·75 per d), hypoxanthine (21·0 (SE 2·58) μmol/kg W0·75 per d) and xanthine (11·2 (SE 0·83) μmol/kg W0·75 per d) were not affected by the experimental treatment, although there was a significant decrease in hypoxanthine excretion in pregnant sows (from 25·5 to 5·2 μmol/kg W0·75 per d) compared with non-pregnant sows (from 26·7 to 44·8 μmol/kg W0·75 per d). Creatinine excretion was not affected by pregnancy and was used as an internal urinary marker. Purine excretion, either expressed as μmol/kg W0·75 per d or as the ratio PD:creatinine, was not affected by experimental treatment, although an apparent increase in pseudouridine excretion, a modified unsalvageable catabolite of RNA-pyrimidine, was found in late pregnancy (3·6 ν 52 mol/100 mol creatinine in on-pregnant sows compared with pregnant sows at 102 d collection).

Keywords

Purine derivativesPseudouridinePregnancySow
Type
Pregnancy and purine metabolism in sows
Information
British Journal of Nutrition , Volume 73 , Issue 3 , March 1995 , pp. 375 - 385
Copyright
Copyright © The Nutrition Society 1995

References

al-Khalidi, U. A. S. & Chaglassian, T. H. (1965) The species distribution of xanthine oxidase. Biochemical Journal 97, 318–320.CrossRefGoogle ScholarPubMed
Agricultural Research Council (1981) The Nutrient Requirements of Pigs. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. & Gasa, J. (1991) Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116, 309–317.CrossRefGoogle Scholar
Balcells, J., Guada, J. A., Castrillo, C. & Gasa, J. (1993) Rumen digestion and urinary excretion of purine derivatives in response to urea supplementation of sodium-treated straw fed to sheep. British Journal of Nutrition 69, 721–732.CrossRefGoogle ScholarPubMed
Balcells, J., Guada, J. A., Peiró, J. M. & Parker, D. S. (1992) Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 575, 153–157.CrossRefGoogle ScholarPubMed
BMDP (1990)In Statistical Software Manual, [Dixon, W.J., editor]. Berkeley, Los Angeles and Oxford: University of California Press.Google Scholar
Borek, E., Baliga, B. S., Gehrke, Ch. W., Kuo, C. W., Belman, S., Troll, W. & Waalkes, T. P. (1977) High turnover rate of transfer RNA in tumor tissue. Cancer Research 37, 3362–3366.Google ScholarPubMed
Borek, E. & Kerr, S. J. (1972) Atypical transfer RNA's and their origin in neoplastic cells. Advances in Cancer Research 16, 163–190.CrossRefGoogle Scholar
Chen, X. B. (1989)Excretion of purine derivatives by sheep and cattle and its use for the estimation of absorbed microbial protein. PhD Thesis, University of Aberdeen.Google Scholar
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. & Brown, D. S. (1990 a) Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1990 b) Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition 63, 121–129.CrossRefGoogle ScholarPubMed
Condon, R. J., Hall, G. & Hatfield, E. E. (1970) Metabolism of abomasally infused 14C labeled ribonucleic acid, adenine, uracil, and glycine. Journal of Animal Science 31, 1037–1038.Google Scholar
Faulkner, A. (1983)Foetal and neonatal metabolism. In Nutritional Physiology of Farm Animals. 1st ed., pp. 203–207 [Rook, J.A. F. and Thomas, P. C., editors]. New York: Longman Inc.Google Scholar
Folin, O., Berglund, H. & Derick, C. (1924) The uric acid problem. An experimental study on animals and man, including gouty subjects. Journal of Biological Chemistry 60, 361–479.CrossRefGoogle Scholar
Free, A. H. & Free, H. M. (1974)Laboratory interrelations of amniotic fluid and urine. In Current Topics in Clinical Chemistry, Vol. 1. Amniotic Fluid: Physiology, Biochemistry and Clinical Chemistry, pp. 37–46 [NatelsonS., S.,, Scommegna, A. and Epstein, M. B., editors]. New York and London: John Wiley and Sons.Google Scholar
Fujihara, T., Ørskov, E. R., Reeds, P. J. & Kyle, D. J. (1987) The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.CrossRefGoogle Scholar
Fujihara, T., Chen, X. B., Ørskov, E. R. & Hovell, F. D. (1988)The possible use of purine derivatives in urine to estimate rumen microbial protein production. In Proceedings of 5th International Symposium on Protein Metabolism and Nutrition. European Association of Animal Production Publication no. 35, pp. 17–18. [Browen, W., editor]. Rostock, Germany: Wilhelm-Pieck University of Rostock.Google Scholar
Gehrke, C. W., Kuo, K. C, Waalkes, T. P. & Borek, E. (1979) Patterns of urinary excretion of modified nucleosides. Cancer Research 39, 1150–1153.Google ScholarPubMed
Giesecke, D., Stangassinger, M. & Tiemeyer, W. (1984) Nucleic acid digestion and urinary purine metabolites in sheep nourished by intragastric infusions. Canadian Journal of Animal Science 64, Suppl., 144–145.CrossRefGoogle Scholar
Greife, H. A. (1980)Nitrogen utilisation of microbial nucleic acids in the growing rat. In Proceedings of the 3rd European Association of Animal Production Symposium on Protein Metabolism and Nutrition. European Association of Animal Production Publication no. 27, pp. 159–167 [Oslage, H.J. and Rohr, K., editors]. Braunschweig, GermanyInstitute of Animal Nutrition.Google Scholar
Hitchings, G. H. (1978)Uric acid: chemistry and synthesis. In Uric Acid, pp. 1–20 [Kelly, W.N. and Weiner, J. M., editors], Berlin, Heidelberg and New York: Springer-Verlag.Google Scholar
Khan, M. S. N., Salim, M. & Maden, B. E. H. (1978) Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal ribonucleic acids. Biochemical Journal 169, 531–542.CrossRefGoogle ScholarPubMed
Lindberg, J. E. (1991) Nitrogen and purine metabolism in preruminant and ruminant goat kids given increasing amounts of ribonucleic acids. Animal Feed Science and Technology 35, 213–226.CrossRefGoogle Scholar
Lindberg, J. E. & Jacobsson, K.-G. (1990) Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion. British Journal of Nutrition 64, 359–370.CrossRefGoogle ScholarPubMed
Martin, S. M., Balcells, J., Guada, J. A. & Castrillo, C. (1993) Endogenous purine and pyrimidine derivative excretion in pregnant sows. Proceedings of the Nutrition Society 52, 211 A.Google Scholar
Murray, A. W. (1971) The biological significance of purine salvage. Annual Review of Biochemistry 40, 811–826.CrossRefGoogle ScholarPubMed
Ørskov, E. R., Grubb, D. A., Wenham, G. & Corrigall, W. (1979) The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acid and protein. British Journal of Nutrition 41, 553–558.CrossRefGoogle ScholarPubMed
Pitkin, R. M. (1974)Changes in osmolality, nonprotein nitrogen, and bilirubin in amniotic fluid during the course of pregnancy. In Current Topics in Clinical Chemistry, Vol. 1. Amniotic Fluid: Physiology, Biochemistry and Clinical Chemistry, pp. 81–94 [Natelson, S., Scommegna, A. and Epstein, M. B., editors]. New York and London: John Wiley and Sons.Google Scholar
Puchala, R., Shelford, J. A., Barej, W., Kulasek, G. W., Piór, H., Keyserlingk, M. V. & Makoni, N. (1993) Urinary excretion of pseudouridine and purine metabolites in ruminants. Journal of Animal Physiology and Animal Nutrition 69, 186–193.CrossRefGoogle Scholar
Razzaque, M. A., Topps, J. H., Kay, R. N. B. & Brockway, J. M. (1981) Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs. British Journal of Nutrition 45, 517–527.CrossRefGoogle ScholarPubMed
Roth, F. X. & Kirchgessner, M. (1979) Utilisation of alimentary ribonucleic acid in calves, N-metabolism. Archiv für Tierernährung 29, 275–283.Google ScholarPubMed
Sander, G., Hülsemann, J., Topp, H., Heller-Schöch, G. & Schöch, G. (1986 a) Protein and RNA turnover in preterm infants and adults: A comparison based on urinary excretion of 3-methylhistidine and of modified one-way RNA catabolites. Annals of Nutrition and Metabolism 30, 137–142.CrossRefGoogle ScholarPubMed
Sander, G., Topp, H., Heller-Schöch, G., Wieland, J. & Schöch, G. (1986 b) Ribonucleic acid turnover in man: RNA catabolites in urine as measure for the metabolism of each of the three major species of RNA. Clinical Science 71, 367–374.CrossRefGoogle Scholar
Schöch, G., Heller-Schöch, G., Müller, J., Heddrich, M. & Grüttner, R. (1982) Determination of RNA metabolism as indicator of nutritional status. Klinische Pädiatrie 194, 317–319.CrossRefGoogle ScholarPubMed
Seeds, A. E. (1974)Dynamics of amniotic fluid. In Current Topics in Clinical Chemistry, Vol. 1. Amniotic Fluid: Physiology, Biochemistry and Clinical Chemistry, pp. 23–36 [Natelson, S., Scommegna, A. and Epstein, M. B., editors]. New York and London: John Wiley and Sons.Google Scholar
Smith, R. C, Moussa, N. M. & Hawkins, G. E. (1974) Utilization of the nucleic acids of Escherichia coli and rumen bacteria by sheep. British Journal of Nutrition 32, 529–537.CrossRefGoogle ScholarPubMed
Steel, R. G. D. & Torrie, J. H. (1980)Principles and Procedures of Statistics, 2nd ed. New York: McGraw-Hill. Technicon Instruments Co. Inc. (1989). Creatinine Technicon Method no. SM4-0141F89. Tarrytown, New York: Technicon Instruments Co. Inc.Google Scholar
Tritsch, G. L., Luch, J. M., Evans, J. T. & Mittelman, A. (1979) Age dependence of human urinary pseudouridine excretion. Biochemical Medicine 22, 387–390.CrossRefGoogle ScholarPubMed
Waterlow, J. C. (1984) Protein turnover with special reference to man. Quarterly Journal of Experimental Physiology 69, 409–438.CrossRefGoogle ScholarPubMed
Weissman, S., Eisen, A. Z., Lewis, M., Karon, M. & Clark, P. (1962) Pseudouridine metabolism. III. Studies with isotopically labeled pseudouridine. Journal of Laboratorial and Clinical Medicine 60, 40–47.Google ScholarPubMed
Zöllner, N. (1982) Purine and pyrimidine metabolism. Proceedings of the Nutrition Society 41, 329–342.CrossRefGoogle ScholarPubMed