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Effects of specific dietary sugars on the incorporation of 13C label from dietary glucose into neutral sugars of rat intestine and serum glycoproteins

Published online by Cambridge University Press:  09 March 2007

Corinne Rambal ,
Christiane Pachiaudi ,
Sylvie Normand ,
Jean-Paul Riou ,
Pierre Louisot  and
Ambroise Martin
Corinne Rambal
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: INSERM U197, Alexis Carrel Medical School, rue Guillaume Paradin, 69008, Lyon, France
Christiane Pachiaudi
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: Department of Biochemistry, INSERM-CNRS U189, Lyon-Sud Medical School, BP12, 69921, Oullins Cedex, France
Sylvie Normand
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: Department of Biochemistry, INSERM-CNRS U189, Lyon-Sud Medical School, BP12, 69921, Oullins Cedex, France
Jean-Paul Riou
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: Department of Biochemistry, INSERM-CNRS U189, Lyon-Sud Medical School, BP12, 69921, Oullins Cedex, France
Pierre Louisot
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: INSERM U197, Alexis Carrel Medical School, rue Guillaume Paradin, 69008, Lyon, France
Ambroise Martin
Affiliation:
Centre de Recherches en Nutrition Humaine de Lyon: INSERM U197, Alexis Carrel Medical School, rue Guillaume Paradin, 69008, Lyon, France
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Abstract

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Although theoretically all glycoprotein sugars can be derived from glucose, it may be hypothesized that specific dietary sugars could be preferential substrates for glycoprotein synthesis. To test this hypothesis, groups of rats received either continuously (continuous-labelling experiment) or for a single nutritional period (pulse-labelling experiment) a 13C-rich diet containing either maize starch or artificially labelled [13C]glucose. Some groups of rats were also provided during a single nutritional period with low amounts (20–200 mg/animal) of low-13C dietary sugars (mannose, galactose, fucose or fructose). If specific dietary sugars were preferentially incorporated into glycoproteins instead nf glucose-derived labelled sugars, a decrease would be expected in the intestinal or serum glycoprotein-sugar 13C enrichment monitored by gas chromatography-isotope-ratio mass spectrometry (GC-IRMS). Contrary to this hypothesis the results showed no significant decrease with any of the specific dietary sugars. Furthermore, with dietary low-13C mannose or galactose, a significant increase in 13C enrichment of glycoprotein-sugars was observed compared with some other nutritional groups. Moreover, in the pulse-labelling experiment, dietary mannose and galactose induced similar patterns of 13C enrichment in intestinal and serum glycoprotein-sugars. Therefore, although specific dietary sugars do not appear to be preferential substrates for glycosylation under conditions and doses relevant to current concepts of nutrition, regulatory roles of some specific dietary sugars in relation to glycoprotein-sugar metabolism might be hypothesized. These findings could lead to similar studies using stable-isotope methodology in man which could have practical consequences, especially in parenteral nutrition where glucose is the only sugar provided to the metabolism.

Keywords

GlycosylationDietary sugarsStable-isotope techniqueGC-IRMS
Type
Glycosylation and specific dietary sugars
Information
British Journal of Nutrition , Volume 73 , Issue 3 , March 1995 , pp. 443 - 454
Copyright
Copyright © The Nutrition Society 1995

References

Bekesi, J. G. & Winzler, R. J. (1967). The metabolism of plasma glycoproteins. Studies on the incorporation of L-fucose-l-14C into tissue and serum in the normal rat. Journal of Biological Chemistry 242, 3873–3879.CrossRefGoogle Scholar
Biol, M. C, Martin, A. & Louisot, P. (1972). Nutritional and developmental regulation of glycosylation processes in digestive organs. Biochimie 74, 13–24.CrossRefGoogle Scholar
Blakeney, A. B., Harris, P. J., Henry, R. J. & Stone, B. A. (1983). A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydrate Research 113, 291–299.CrossRefGoogle Scholar
Bocci, V. & Winzler, R. J. (1969). Metabolism of L-fucose-l-14C and of fucose glycoproteins in the rat. American Journal of Physiology 216, 1337–1342.CrossRefGoogle Scholar
Brossard, N., Pachiaudi, C, Croset, M., Normand, S., Lecersg, J., Chirouze, V., Riou, J. P., Tayot, J. L. & Lagarde, M. (1994). Stable isotope tracer and gas chromatography-combustion isotope ratio mass spectrometry to study the in vivo compartmental metabolism of docosahexaenoic acid. Analytical Biochemistry 220,192–199.CrossRefGoogle Scholar
Brydon, W. G., Merrick, M. V. & Hannan, J. (1987). Absorbed dose from 14C xylose and 14C mannose. British Journal of Radiology 60, 563–566.CrossRefGoogle ScholarPubMed
Capps, J. C, Shetlar, M. R. & Bradford, R. H. (1966). Hexosamine metabolism. The absorption and metabolism in vivo of orally administered D-glucosamine and N-acetyl-D-glucosamine in the rat. Biochimica et Biophysica Acta 127, 194–204.CrossRefGoogle Scholar
Coffey, J. W., Neal Miller, O. & Sellinger, O. Z. (1964). The metabolism of L-fucose in the rat. Journal of Biological Chemistry 239, 4011–4017.CrossRefGoogle Scholar
Craig, G. H. (1957). Isotopic standards for C and O and correction factors for mass spectrometry analysis of carbon dioxide. Geochimica Cosmochimica Acta 12, 133–149.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane-Stanley, G. M. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Garlick, P. J., Wenerman, J., McNurlan, M. A., Essen, P., Lobley, G. E., Milne, E., Calder, G. A. & Vinnars, E. (1989). Measurement of the rate of protein synthesis in muscle of post-absorptive young men by injection of a flooding dose of 1–13C leucine. Clinical Science 77, 329–336.CrossRefGoogle Scholar
Ginsburg, V. (1961). Studies on the biosynthesis of guanosine diphosphate L-fucose. Journal of Biological Chemistry 236, 2389–2393.CrossRefGoogle ScholarPubMed
Goodman, K. J. & Brenna, J. T. (1992). High sensitivity tracer detection using high precision gas chromatography-combustion isotope ratio mass spectrometry and highly enriched [U13C] labeled precursors. Analytical Chemistry 64, 1088–1095.CrossRefGoogle ScholarPubMed
Henry, R. J., Blakeney, A. B., Harris, P. J. & Stone, B. A. (1983). Detection of neutral and aminosugars from glycoproteins and polysaccharides as their alditol acetates. Journal of Chromatography 256, 419–427.CrossRefGoogle ScholarPubMed
Kornfeld, R. & Kornfeld, S. (1985). Assembly of asparagine-linked oligosaccharides. Annual Review of Biochemistry 54, 631–664.CrossRefGoogle ScholarPubMed
McClain, D. A., Paterson, A. J., Roos, M. D. & Kudlow, J. E. (1992). Glucose and glucosamine regulate growth factor gene expression in vascular smooth muscle cells. Proceedings of the National Academy of Sciences, USA 89, 8150–8154.CrossRefGoogle ScholarPubMed
Miyamoto, K., Hase, K., Takagi, T., Fujii, T., Taketani, Y., Minami, H., Oka, T. & Nakabou, Y. (1993). Differential responses of intestinal glucose transporter mRNA transcripts to levels of dietary sugars. Biochemical Journal 295, 211–215.CrossRefGoogle ScholarPubMed
Normand, S., Pachiaudi, C, Khalfallah, Y., Guilluy, R., Mornex, R. & Riou, J. P. (1992). 13C appearance in plasma glucose and breath C02 during feeding with naturally 13C enriched starchy foods in normal humans. American Journal of Clinical Nutrition 55, 430–435.CrossRefGoogle Scholar
Rambal, C, Pachiaudi, C, Normand, S., Riou, J. P., Louisot, P. & Martin, A. (1992). Use of compounds naturally enriched with stable isotopes for the study of the metabolism of glycoprotein neutral sugars by gas chromatography-isotope ratio mass spectrometry. Technical validation in the rat. Carbohydrate Research 236, 29–37.CrossRefGoogle Scholar
Robinson, G. B. (1967). The assimilation and metabolism of glucosamine. Biochemical Journal 104, 61P.Google ScholarPubMed
Robinson, G. B. (1968). Distribution of isotopic label after the oral administration of free and bound 14C-labeled glucosamine in rats. Biochemical Journal 108, 275–280.CrossRefGoogle Scholar
Shetlar, M. R., Capps, J. C. & Hern, D. L. (1964). Incorporation of radioactive glucosamine into serum proteins of intact rats and rabbits. Biochimica et Biophysica Acta 83, 93–101.Google ScholarPubMed
Tauber, R., Park, C. S., Becker, A., Geyer, R. & Reutter, W. (1989). Rapid intramolecular turn-over of N-linked glycans in plasma membrane glycoproteins. European Journal of Biochemistry 186, 55–62.CrossRefGoogle Scholar
Tissot, S., Normand, S., Guilluy, R., Pachiaudi, C, Beylot, M., Laville, M., Cohen, R., Mornex, R. & Riou, J. P. (1990). Use of a new gas chromatograph isotope ratio mass spectrometer to trace exogenous 13C labelled glucose at a very low level of enrichment in man. Diabetologia 33, 449–456.CrossRefGoogle Scholar
West, C. M. (1986). Current ideas on the significance of protein glycosylation. Molecular and Cellular Biochemistry 72, 3–20.CrossRefGoogle ScholarPubMed
White, B. N., Shetlar, M. R., Shurley, H. M. & Schilling, J. A. (1965). Incorporation of D-[l-14C]galactosamine into serum proteins and tissues of the rat. Biochimica et Biophysica Acta 101, 259–266.CrossRefGoogle Scholar
Wood, F. C. & Cahill, G. F. (1963). Mannose utilization in man. Journal of Clinical Investigation 42, 1300–1311.CrossRefGoogle ScholarPubMed