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Effects of solvent extraction on the hypocholesterolaemic action of oat bran in the rat

Published online by Cambridge University Press:  09 March 2007

Richard J. Illman ,
David L. Topping ,
Kerin Dowling ,
Rodney P. Trimble ,
Gordon R. Russell  and
Gerald B. Storer
Richard J. Illman
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
David L. Topping
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
Kerin Dowling
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
Rodney P. Trimble
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
Gordon R. Russell
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
Gerald B. Storer
Affiliation:
CSIRO (Australia) Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, South Australia 5158, Australia
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Abstract

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In adult male rats fed on a cholesterol-free synthetic diet, plasma cholesterol concentrations were lowest with oat bran, intermediate with cellulose and highest with wheat bran. Plasma triacylglycerols (TAG) were similar with wheat bran and cellulose but higher with oat bran. The concentrations and pools of caecal volatile fatty acids (VFA) were lowest with cellulose and equally higher with oat bran and wheat bran. Plasma VFA concentrations in the hepatic portal vein reflected those in caecal digesta and were unrelated to plasma cholesterol. Feeding oat bran after extraction with n-pentane gave plasma cholesterol concentrations similar to that found with wheat bran. Reconstitution of oat bran with extracted lipids did not restore the cholesterol-lowering effect. Addition of the extracted material to a wheat-bran diet had no effect on plasma cholesterol. Plasma TAG were higher with the oat bran and reconstituted-oatbran diets than with wheat-bran or cellulose diets. However, extracted oat bran+safflower oil gave similar TAG concentrations to that with wheat bran. These extractions and additions did not change caecal bile acid or neutral sterol concentrations. Effects of these diets on plasma cholesterol were unrelated to their tocotrienol or tocopherol content. Addition of n-pentane to oat bran followed by evaporation of solvent gave plasma cholesterol concentrations that were significantly higher than untreated oat bran but lower than similarly treated wheat bran. It is concluded that oat bran affects cholesterol metabolism through a pentane-soluble component as well as non-starch polysaccharides. It appears that the activity of this lipid is not transferable by simple addition of the solvent extract to the whole diet.

Keywords

HypocholesterolaemiaOat branRat
Type
Effects of Dietry Fibre on Lipid Metabolism
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 435 - 443
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Anderson, J. W., Story, L., Seiling, B., Chen, W.-J. L., Petro, M. & Story, S. (1984). Hypocholesterolemic effects of oat-bran or bean intake for hypocholesterolemic men. American Journal of Clinical Nutrition 40, 1146–1155.CrossRefGoogle ScholarPubMed
Arbeeny, C. M., Rifici, V. A. & Eder, H. A. (1987). The uptake of the apoprotein and cholesteryl ester of high-density lipoproteins by the perfused rat liver. Biochimica et Biophysica Acta 917, 9–17.CrossRefGoogle ScholarPubMed
Asp, N.-G., Bauer, H. G., Nilsson-Ehle, P., Nyman, M. & Oste, R. (1981). Wheat bran increases high-density-lipoprotein cholesterol in the rat. British Journal of Nutrition 46, 385–393.CrossRefGoogle ScholarPubMed
Chen, W.-J., Anderson, J. W. & Gould, M. R. (1981). Effects of oat bran, oat gum and pectin on lipid metabolism of cholesterol-free rats. Nutrition Reports International 24, 1093–1098.Google Scholar
Cheng, B.-Q., Trimble, R. P., Illman, R. J., Stone, B. A. & Topping, D. L. (1987). Comparative effects of dietary wheat bran and its morphological components (aleurone and pericarp-seed coat) on volatile fatty acid concentrations in the rat. British Journal of Nutrition 57, 69–76.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Cummings, J. H. (1988). Improved method of measurement of dietary fibre as non-starch polysaccharides in plant food. Journal of the Association of Official Analytical Chemists 71, 808–814.Google Scholar
Hatam, L. J. & Kayden, H. J. (1979). A high-performance liquid chromatographic method for the determination of tocopherol in plasma and cellular elements of the blood. Journal of Lipid Research 20, 639–645.CrossRefGoogle ScholarPubMed
Illman, R. J. & Topping, D. L. (1985). Effects of dietary oat bran on faecal steroid excretion, plasma volatile acids and lipid synthesis in rats. Nutrition Research 5, 839–846.CrossRefGoogle Scholar
Illman, R. J., Topping, D. L., McIntosh, G. H., Trimble, R. P., Storer, G. B., Taylor, M. N. & Cheng, B.-Q. (1988). Hypocholesterolaemic effects of dietary propionate: studies in whole animals and perfused rat liver. Annals of Nutrition and Metabolism 32, 97–107.CrossRefGoogle ScholarPubMed
Judd, P. & Truswell, A. S. (1981). The effect of rolled oats on blood lipids and fecal steroid excretion in man. American Journal of Clinical Nutrition 34, 2061–2067.CrossRefGoogle ScholarPubMed
Kestin, M., Moss, R., Clifton, P. M. & Nestel, P. J. (1990). The comparative effects of three cereal brans on plasma lipids, blood pressure and glucose metabolism in mildly hypercholesterolemic men. American Journal of Clinical Nutrition 52, 661–666.CrossRefGoogle ScholarPubMed
Kirby, R. W., Anderson, J. W., Sieling, B., Rees, E. D., Chen, W.-J., Miller, R. & Kay, R. M. (1981). Oat bran selectively lowers serum low density lipoprotein cholesterol concentrations of hypercholesterolemic men. American Journal of Clinical Nutrition 34, 824–829.CrossRefGoogle ScholarPubMed
Klopfenstein, C. F. & Hoseney, R. C. (1987). Cholesterol-lowering effect of beta-glucan-enriched bread. Nutrition Reports International 26, 1091–1098.Google Scholar
Nestel, P. J. & Grundy, S. M. (1976). Changes in plasma triglyceride secretion during withdrawal of bile. Metabolism 25, 1259–1268.CrossRefGoogle ScholarPubMed
Nyman, M. & Asp, N.-G. (1985). Dietary fibre fermentation in the rat intestinal tract: effect of adaptation period, protein and fibre levels and particle size. British Journal of Nutrition 54, 635–643.CrossRefGoogle ScholarPubMed
Qureshi, A. A., Burger, W. C., Peterson, D. M. & Elson, C. E. (1986). The structure of an inhibitor of cholesterol biosynthesis isolated from barley. Journal of Biological Chemistry 261, 10544–10551.CrossRefGoogle ScholarPubMed
Roach, P. D., Kambouris, A., Topping, D. L., Trimble, R. P. & Nestel, P. J. (1987). The effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat. FEBS Letters 222, 159–162.CrossRefGoogle ScholarPubMed
Schinnick, F. L., Longacre, M. L., Ink, S. L. & Marlett, J. A. (1988). Oat fibre; composition vs. physiological function. Journal of Nutrition 118, 144–151.CrossRefGoogle Scholar
Schneeman, B. O. (1986). Dietary fibre: physical and chemical properties, methods of analysis and physiological effects. Food Technology 40, 104–110.Google Scholar
Theander, O. & Westerlund, E. A. (1986). Studies on dietary fibre. 3. Improved procedures for analysis of dietary fibre. Journal of Agricultural and Food Chemistry 34, 330–336.CrossRefGoogle Scholar
Topping, D. L., Illman, R. J., Roach, P. D., Trimble, R. P., Kambouris, A. & Nestel, P. J. (1990). Modulation of the hypolipidemic effect of fish oils by dietary fibre in rats: studies with rice and wheat bran. Journal of Nutrition 120, 325–330.CrossRefGoogle ScholarPubMed
Topping, D. L., Oakenfull, D., Trimble, R. P. & Illman, R. J. (1988). A viscous fibre (methylcellulose) lowers blood glucose and plasma triacylglycerols and increases liver glycogen independently of volatile fatty acid production in the rat. British Journal of Nutrition 59, 21–30.CrossRefGoogle ScholarPubMed
van Horn, L. V., Liu, K., Parker, D., Emidy, L., Liao, Y., Pan, W. H., Giumetti, D., Hewitt, J. & Stamler, J. (1986). Serum lipid response to oat product intake with a fat-modified diet. Journal of the American Dietetic Association 86, 759–764.CrossRefGoogle ScholarPubMed