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Effects of orally administered Lactobacillus casei DN-114 001 on the composition or activities of the dominant faecal microbiota in healthy humans

Published online by Cambridge University Press:  08 March 2007

Violaine Rochet ,
Lionel Rigottier-Gois ,
Maléne Sutren ,
Marie-Noëlle Krementscki ,
Claude Andrieux ,
Jean-Pierre Furet ,
Patrick Tailliez ,
Florence Levenez ,
Agnés Mogenet  and
Jean-Louis Bresson
...Show all authors
Violaine Rochet*
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Lionel Rigottier-Gois
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Maléne Sutren
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Marie-Noëlle Krementscki
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Claude Andrieux
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Jean-Pierre Furet
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Patrick Tailliez
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Florence Levenez
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
Agnés Mogenet
Affiliation:
Centre d'Investigation Clinique AP-HP/Inserm, Hôpital Necker – Enfants Malades et Université Paris V, Paris, France
Jean-Louis Bresson
Affiliation:
Centre d'Investigation Clinique AP-HP/Inserm, Hôpital Necker – Enfants Malades et Université Paris V, Paris, France
Séverine Méance
Affiliation:
Danone Vitapole, 91 767 Palaiseau Cedex, France
Chantal Cayuela
Affiliation:
Danone Vitapole, 91 767 Palaiseau Cedex, France
Antony Leplingard
Affiliation:
Danone Vitapole, 91 767 Palaiseau Cedex, France
Joël Dore
Affiliation:
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Systéme Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France
*
*corresponding author: Dr V. Rochet, fax +33 1 34 65 24 92, email [email protected]
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Abstract

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The composition and activities of the faecal microbiota in twelve healthy subjects analysed in a single open study were monitored before (1-week baseline step), during (10d supplementation step) and after (10d follow-up step) the ingestion of a fermented milk containing Lactobacillus casei DN-114001. Fluorescent in situ hybridisation with group-specific DNA probes, real-time PCR using L. paracasei group-specific primers and temporal temperature gradient gel electrophoresis (TTGE) using group-specific primers were carried out, together with bacterial enzyme activity and metabolite analyses to monitor the structure and activities of the faecal microbiota. L. casei DNA was detected in the faeces of all of the subjects by TTGE after 10d supplementation. Its quantification by real-time PCR showed a 1000-fold increase during the test step compared with initial levels. No major modification in either the dominant members of the faecal microbiota or their activities was observed during the trial. In conclusion, the short-term consumption of a milk product containing L. casei DN-114001 was accompanied by a high, transient increase in the quantity of this strain in the faeces of all of the subjects without markedly affecting biochemical or bacteriological factors.

Keywords

Human faecal microbiotaProbioticLactobacillus caseiReal-time PCRFluorescent in situ hybridisation
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 421 - 429
Copyright
Copyright © The Nutrition Society 2006

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