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Effects of mild zinc deficiency, plus or minus an acute-phase response, on galactosamine-induced hepatitis in rats

Published online by Cambridge University Press:  09 March 2007

Susan E. Parsons  and
Robert A. Disilvestro
Susan E. Parsons
Affiliation:
Human Nutrition and Food Management, Ohio State University, 265 Campbell Hall, 1787 Neil Avenue, Columbus, OH 43210, USA
Robert A. Disilvestro
Affiliation:
Human Nutrition and Food Management, Ohio State University, 265 Campbell Hall, 1787 Neil Avenue, Columbus, OH 43210, USA
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Abstract

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Zn deficiency is hypothesized to produce poor resistance to injury involving oxidative stress. This could occur by impairing Zn antioxidant function(s) or by indirectly limiting adaptive protective mechanisms such as a rise in acute-phase proteins. The present study examined rats fed diets adequate or moderately low in Zn (4 or 25 μg/g diet) for 9 d. The lower intake produced a mild Zn deficiency based on body weight, plasma Zn and plasma alkaline phosphatase (EC 3.1.3.1) activity. Galactosamine injection, an oxidative stress, produced much more liver injury in the mildly Zn-deficient rats. However, injury was strongly inhibited in rats from each dietary group by an acute-phase response due to turpentine-induced leg inflammation. Mild Zn deficiency did not prevent a rise in levels of the acute-phase protein caeruloplasmin (EC 1.16.3.1), but did limit the usual inflammation-induced rise in hepatic levels of metallothionein, a Zn protein with possible antioxidant function. In conclusion, high degrees of galactosamine-induced hepatitis were associated with mild Zn deficiency, but the liver injury was blocked by prior stimulation of an acute-phase response, regardless of Zn status.

Keywords

ZincGalactosamine-induced hepatitisMetallothionein
Type
Zinc deficiency and the acute phase response
Information
British Journal of Nutrition , Volume 72 , Issue 4 , October 1994 , pp. 611 - 618
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Alcorn, J. M., Fierer, J. & Chojkier, M. (1992). The acute-phase response protects mice from D-galactosamine sensitization to endotoxin and tumor necrosis factor-a. Hepatology 15, 122–129.CrossRefGoogle Scholar
Blalock, L. T., Dunn, M. A. & Cousins, R. J. (1988). Metallothionein gene expression in rats: tissue specific regulation by dietary copper and zinc. Journal of Nutrition 118, 222–228.CrossRefGoogle ScholarPubMed
Blatteis, C. M. (1985). Central nervous system effects of interleukin-I. In The Physiologicai, Metabolic, and Immunological Actions of Interleukin-1, pp. 107–120 [Kluger, M. J., Oppenheim, J. J. and Powanda, M. C., editors]. New York: Alan R. Liss, Inc.Google Scholar
Bray, T. M. & Bettger, W. J. (1990). The physiological role of zinc as an antioxidant. Free Radical Biology and Medicine 8, 281–291.CrossRefGoogle ScholarPubMed
Chojkier, M. & Fierer, J. (1985). D-Gdactosamine hepatotoxicity is associated with endotoxin sensitivity and mediated by lymphoreticular cells in mice. Gastroenterology 88, 115–121.CrossRefGoogle ScholarPubMed
Cousins, R. J. (1985). Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiological Reviews 65, 238–309.CrossRefGoogle ScholarPubMed
Crapo, J. D., Barry, B. E., Foscue, H. A. & Shelburne, J. (1980). Structural and biochemical changes in rat lungs occurring during exposures to lethal and adaptive doses of oxygen. American Review of Respiratory Diseases 122, 123–143Google ScholarPubMed
Decker, K. & Keppler, D. (1974). Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Reviews of Physiology, Biochemistry and Pharmacology 71, 78–102.Google Scholar
DiSilvestro, R. A., Barber, E. F., David, E. A. & Cousins, R. J. (1988). An enzyme linked immunoadsorbent assay for rat ceruloplasmin. Biological Trace Element Research 17, 1–9.CrossRefGoogle ScholarPubMed
DiSilvestro, R. A. & Carlson, G. P. (1994). Effects of mild zinc deficiency, plus or minus acute phase response, on CCI, hepatotoxicity. Free Radical Biology & Medicine 16, 57–61.CrossRefGoogle ScholarPubMed
Eaton, D. L. & Toal, B. F. (1982). Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicology and Applied Pharmacology 66, 134–142.CrossRefGoogle ScholarPubMed
Farghali, H., Gasbarrini, A., Borle, A. B., Nalesnik, M. A., Francavilla, A., Fagiuoli, S., Starzi, T. E. & Van Thiel, D. H. (1991). FK 506 modulates D-galactosamine-induced hepatitis in rats. Transplantation Proceedings 23, 2809–2811.Google ScholarPubMed
Korsrud, G. O., Grice, H. C. & McLaughlan, J. M. (1972). Sensitivity of several serum enzymes in detecting carbon tetrachloride-induced liver damage in rats. Toxicology and Applied Pharmacology 22, 414–483.CrossRefGoogle ScholarPubMed
Kubow, S., Bray, T. M. & Bettger, W. J. (1986). Effects of dietary zinc and copper on free radicalproduction in rat lung and liver. Canadian Journal of Physiology and Pharmacology 64, 1281–1285.CrossRefGoogle Scholar
Luecke, R. W., Olman, M. E. & Baltzer, B. U. (1968). Zinc deficiency in the rat: effect on serum and intestinal alkaline phosphatase activities. Journal of Nutrition 94, 344–350.CrossRefGoogle ScholarPubMed
Ohta, H., Ono, M., Sekiya, C. & Namiki, N. (1992). Serum immunoreactive, & glucuronidase determined by an enzyme-linked immunosorbent assay in patients with hepatic diseases. Clinica Chimica Acta 208, 9–21.CrossRefGoogle ScholarPubMed
Platt, D., Forster, K. & Forster, L. (1978). Age dependent kinetic studies of cytoplasmic and lysosomal enzymes of the normal and D-galactosamine injured rat liver. Mechanisms of Ageing and Development 7, 183–188.CrossRefGoogle ScholarPubMed
Rice, E. W. (1962). Standardization of ceruloplasmin activity in terms of international enzyme units. Analytical Biochemistry 3, 452–456.CrossRefGoogle ScholarPubMed
Sato, M. & Bremner, I. (1993). Oxygen free radicals and metallothionein. Free Radical Biology and Medicine 14, 325–327.CrossRefGoogle ScholarPubMed
Shedlofsky, S. I. & McClain, C. J. (1991). Hepatic dysfunction due to cytokines. In Cytokines and Inflammation, pp. 235–273 [Kimbal, E. S., editor]. Boca Ration: CRC Press.Google Scholar
Steel, R. D. G. & Torrie, J. H. (1980). Principles and Procedures of Statistics, A Biometrical Approach, 2nd ed. New York: McGraw-Hill.Google Scholar
Sullivan, J. F., Jetton, M. M., Hahn, H. K. J. & Burch, R. E. (1980). Enhanced lipid peroxidation in liver microsomes of zinc-deficient rats. American Journal of Clinical Nutrition 33, 51–56.CrossRefGoogle ScholarPubMed
Taylor, C. G. & Bray, T. M. (1991). Effect of hyperoxia on oxygen free radical defense enzymes in the lung of zinc- deficient rats. Journal of Nutrition 121, 460–466.CrossRefGoogle ScholarPubMed
Taylor, C. G., Tower, R. A., Janzen, E. G. & Bray, T. M. (1990). MRI detection of hyperoxia-induced lung edema in Zn-deficient rats. Free Radical Biology and Medicine 9, 229–233.CrossRefGoogle ScholarPubMed
Tsuda, H., Wada, S., Masui, T., Inui, M., Ito, N., Katagiri, K., Hoshino, M., Inaguma, H., Miyagi, M. & Takeuchi, T. (1990). Comparative sequential changes in serum and biliary levels of bile acid components after a single dose of D-galactosamine or partial hepatectomy in the rat. Liver 10, 28–34.CrossRefGoogle ScholarPubMed