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The effects of fasting on the thermogenic, metabolic and cardiovascular responses to infused adrenaline

Published online by Cambridge University Press:  09 March 2007

J. Webber ,
J. Taylor ,
H. Greathead ,
J. Dawson ,
P. J. Buttery  and
I.A. MacDonald
J. Webber
Affiliation:
Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham NG7 2UH
J. Taylor
Affiliation:
Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham NG7 2UH
H. Greathead
Affiliation:
Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough, University of Nottingham, Leicestershire LE12 5RD
J. Dawson
Affiliation:
Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough, University of Nottingham, Leicestershire LE12 5RD
P. J. Buttery
Affiliation:
Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough, University of Nottingham, Leicestershire LE12 5RD
I.A. MacDonald
Affiliation:
Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham NG7 2UH
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Abstract

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The effects of fasting on the thermogenic, lipolytic and cardiovascular responses to adrenaline were examined in nine normal, young, non-obese subjects. Each subject attended for study after 12, 36 and 72 h fasting. After basal measurements adrenaline was infused at 25 ng/min per kg ideal body weight for 90 min. Fasting increased the thermogenic effect of the adrenaline (mean 14·6 (SE 1·7), 16·6 (SE 1·8), 22·6 (SE 1·6) J/:min per kg fat-free mass after 12, 36 and 72 h fasting respectively; P < 0·001, ANOVA). Basal plasma palmitate turnover increased with duration of fasting (1·48 (SE 0·22), 1·95 (SE 0·34) and 2·26 (SE 0·33) μmol/min per kg body weight: P < 0·001, ANOVA), but the response to adrenaline was unaffected by fasting. The percentage values for basal plasma palmitate turnover oxidized were 44 (SE 2; 12 h), 46 (SE 5; 36 h) and 42 (SE 4)% (72 h). In response to adrenaline this percentage fell, suggesting that adrenaline infusion may favour intra-tissue lipid oxidation.

Keywords

Sympathetic nervous systemMetabolic rateHeart rateLipolysis[1-13C]palmitate
Type
Effects of fasting on responses to adrenaline
Information
British Journal of Nutrition , Volume 74 , Issue 4 , October 1995 , pp. 477 - 490
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Allsop, J. R., Wolfe, R. R. & Burke, J. F. (1978). Tracer priming the bicarbonate pool. American Journal of Physiology 45, 137–139.Google Scholar
Bearn, A. G., Billing, B. & Sherlock, S. (1951). The effect of adrenaline and noradrenaline on hepatic blood flow and splanchnic carbohydrate metabolism in man. Journal of Physiology 115, 430–441.CrossRefGoogle ScholarPubMed
Dagenais, G. R., Tancredi, R. D. & Zierler, K. L. (1976). Free fatty acid oxidation by forearm muscle at rest, and evidence for an intramuscular lipid pool in the human forearm. Journal of Clinical Investigation 58, 421–431.Google Scholar
Durin, J. V. G. A. & Womersley, J. (1974). Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. British Journal of Nutrition 32, 77–97.CrossRefGoogle Scholar
Fellows, I. W. & Macdonald, I. A. (1985). An automated method for the measurement of oxygen consumption and carbon dioxide excretion in man. Clinical Physics and Physiological Measurement 6, 349–355.CrossRefGoogle ScholarPubMed
Hachey, D. L., Patterson, B. W., Reeds, P. J. & Elsas, L. J. (1991). Isotope determination of organic keto acid pentafluorobenzyl esters in biological fluids by negative chemical ionization gas chromatography/mass spectrometry. Analytical Chemistry 63, 919–923.Google Scholar
Heiling, V. J., Miles, J. M. & Jensen, M. D. (1991). How valid are isotopic measurements of fatty acid oxidation? American Journal of Physiology 261, E572–E577.Google ScholarPubMed
Jayarajan, M. P. & Shetty, P. S. (1987). Cardiovascular β-adrenoceptor sensitivity of undernourished subjects. British Journal of Nutrition 58, 5–11.CrossRefGoogle ScholarPubMed
Jensen, M. D., Haymond, M. W., Gerich, J. E., Cryer, P. E. & Miles, J. M. (1987). Lipolysis during fasting decreased suppression by insulin and increased stimulation by epinephrine. Journal of Clinical Investigation 79, 207–213.CrossRefGoogle ScholarPubMed
Jensen, M. D., Heiling, V. & Miles, J. M. (1990). Measurement of non-steady state free fatty acid turnover. American Journal of Physiology 258, E103–E108.Google ScholarPubMed
Jequier, E., Acheson, K. & Schutz, Y. (1987). Assessment of energy expenditure and fuel utilization in man. Annual Review of Nutrition 7, 187–208.Google Scholar
Klein, S., Holland, O. B. & Wolfe, R. R. (1990). Importance of blood glucose concentration in regulating lipolysis during fasting in humans. American Journal of Physiology 258, E32–E39.Google Scholar
Klein, S., Peters, E. J., Holland, O. B. & Wolfe, R. R. (1989). Effect of short- and long-term β-adrenergic blockade on lipolysis during fasting in humans. American Journal of Physiology 257, E65–E73.Google Scholar
Klein, S., Sakurai, Y., Romijn, J. A. & Carroll, R. M. (1993). Progressive alterations in lipid and glucose metabolism during short-term fasting in young adult men. American Journal of Physiology 265, E801–E806.Google Scholar
Knox, D. P. & Jones, D. G. (1984). Automated enzymatic determination of plasma free fatty acids by centrifugal analysis. Journal of Automatic Chemistry 6, 152–154.Google Scholar
Kurpad, A. V., Kulkarni, R. N., Sheela, M. L. & Shetty, P. S. (1989). Thermogenic responses to graded doses of noradrenaline in undernourished Indian male subjects. British Journal of Nutrition 61, 201–208.CrossRefGoogle ScholarPubMed
Landsberg, L. & Young, J. B. (1978). Fasting, feeding and regulation of the sympathetic nervous system. New England Journal of Medicine 298, 1295–1301.CrossRefGoogle ScholarPubMed
Livesey, G. & Elia, M. (1988). Estimation of energy expenditure, net carbohydrate utilization, and net fat oxidation and synthesis by indirect calorimetry: evaluation of errors with special reference to the detailed composition of fuels. American Journal of Clinical Nutrition 47, 608–628.Google Scholar
Lloyd, B., Burrin, J., Smythe, P. & Alberti, K. G. M. M. (1978). Enzymatic fluorometric continuous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol and 3-hydroxybutyrate. Clinical Chemistry 34,1724–1729.Google Scholar
Lundholm, L. & Svedmyr, N. (1965). Influence of adrenaline on blood flow and metabolism in the human forearm. Acta Physiologica Scandinavica 65, 344–351.Google Scholar
Macdonald, I. A. & Lake, D. M. (1985). An improved technique for extracting catecholamines from body fluids. Journal of Neuroscience Methods 13, 239–248.Google Scholar
McGiuire, E. A. H., Helderman, J. H., Tobin, J. D., Andres, R. & Berman, M. (1976). Effects of arterial versus venous sampling on analysis of glucose kinetics in man. Journal of Applied Physiology 41, 565–573.CrossRefGoogle Scholar
Mansell, P. I., Fellows, I. W. & Macdonald, I. A. (1990). 48 h starvation enhances the thermogenic response to infused adrenaline. American Journal of Physiology 258, R87–R93.Google Scholar
Mansell, P. I. & Macdonald, I. A. (1989). Underfeeding and the physiological responses to infused epinephrine in lean women. American Journal of Physiology 256, R583–R589.Google ScholarPubMed
Metcalfe, H. K., Monson, J. P., Welch, S. G. & Cohen, R. D. (1986). Inhibition of lactate removal by ketone bodies in rat liver: evidence for a quantitatively important role of the plasma membrane lactate transporter in lactate metabolism. Journal of Clinical Investigation 78, 743–747.Google Scholar
Nilsson, L. H. & Hultman, E. (1973). Liver glycogen in man - the effect of total starvation or a carbohydrate-poor diet followed by carbohydrate refeeding. Scandinavian Journal of Clinical and Laboratory Investigation 32, 325–330.Google Scholar
O'Dea, K., Esler, M., Leonard, P., Stockigt, J. R. & Nestel, P. (1982). Noradrenaline turnover during under- and over-eating in normal weight subjects. Metabolism 31, 896–899.CrossRefGoogle ScholarPubMed
Piolino, V., Acheson, K. J., Muller, M. J., Jeanpretre, N., Burger, A. G. & Jequier, E. (1990). Thermogenic effect of thyroid hormones: interactions with epinephrine and insulin. American Journal of Physiology 259, E305–E311.Google Scholar
Sacca, L., Vigorito, C., Cicala, M., Corso, G. & Sherwin, R. S. (1983). Role of gluconeogenesis in epinephrine-stimulated hepatic glucose production in humans. American Journal of Physiology 245, E294–E302.Google Scholar
Simonsen, L., Bulow, J., Madsen, J. & Christensen, N. J. (1992). Thermogenic response to epinephrine in the forearm and abdominal subcutaneous adipose tissue. American Journal of Physiology 263, E850–E855.Google Scholar
Staten, M. A., Matthews, D. E., Cryer, P. E. & Bier, D. M. (1989). Epinephrine's effect on metabolic rate is independent of changes in plasma insulin or glucagon. American Journal of Physiology 257, E185–E192.Google Scholar
Vaughan, M. (1961). The metabolism of adipose tissue in vitro. Journal of Lipid Research 2, 293–316.Google Scholar
Webb, P. (1986). 24-hour energy expenditure and the menstrual cycle. American Journal of Clinical Nutrition 44, 614–619.CrossRefGoogle ScholarPubMed
Webber, J. & Macdonald, I. A. (1994). The cardiovascular, metabolic and hormonal changes accompanying acute starvation in men and women. British Journal of Nutrition 71, 437–447.Google Scholar
Webber, J., Simpson, E., Parkin, H. & Macdonald, I. A. (1994). Metabolic effects of acute hyperketonaemia in man before and during an hyperinsulinaemic euglycaemic clamp. Clinical Science 86, 677–687.Google Scholar
Weir, J. B. de V. (1949). New methods of calculating metabolic rate with special reference to protein metabolism. Journal of Physiology (London) 109, 1–9.Google Scholar
Wenham, D., Pacy, P. J., Halliday, D., Price, G. M. & Millward, D. J. (1991). Bicarbonate recovery: feeding versus time. Proceedings of the Nutrition Society 50, 47A.Google Scholar
Whitney, R. J. (1953). The measurement of volume changes in human limbs. Journal of Physiology (London) 121, 1–27.Google Scholar
Wolfe, R. R., Evans, J. E., Mullany, C. J. & Burke, J. F. (1980). Measurement of plasma free fatty acid turnover and oxidation using [l-13C]palmitic acid. Biomedical Mass Spectrometry 7, 168–171.Google Scholar
Wolfe, R. R., Peters, E. J., Klein, S., Holland, O. B., Rosenblatt, J. & Gary, H. Jr (1987). Effect of short-term fasting on lipolytic responsiveness in normal and obese human subjects. American Journal of Physiology 252, E189–E196.Google Scholar