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Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves

Published online by Cambridge University Press:  09 March 2007

Sylvia H. Duncan ,
E. Carol McWilliam Leitch ,
Karen N. Stanley ,
Anthony J. Richardson ,
Richard A. Laven ,
Harry J. Flint  and
Colin S. Stewart
Sylvia H. Duncan*
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
E. Carol McWilliam Leitch
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
Karen N. Stanley
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
Anthony J. Richardson
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
Richard A. Laven
Affiliation:
Scottish Agricultural College, St Mary's Industrial Estate, Dumfries DG1 1DX, UK
Harry J. Flint
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
Colin S. Stewart
Affiliation:
Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
*
*Corresponding author: Dr S. H. Duncan, fax +44 1224 716687, email [email protected]
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Abstract

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The human pathogen Escherichia coli O157:H7 is thought to be spread by direct or indirect contact with infected animal or human faeces. The present study investigated the effects of the plant coumarin esculin and its aglycone esculetin on the survival of a strain of E. coli O157 under gut conditions. The addition of these compounds to human faecal slurries and in vitro continuous-flow fermenter models simulating conditions in the human colon and rumen caused marked decreases in the survival of an introduced strain of E. coli O157. When four calves were experimentally infected with E. coli O157 and fed esculin, the pathogen was detected in five of twenty-eight (18 %) of faecal samples examined post-inoculation, compared with thirteen of thirty-five (37 %) of faecal samples examined from five control calves not fed esculin. Coumarin compounds that occur naturally in dietary plants or when supplemented in the diet probably inhibit the survival of E. coli O157 in the gut.

Keywords

Plant coumarinsEscherichia coli O157RumenColon
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 5 , May 2004 , pp. 749 - 755
Copyright
Copyright © The Nutrition Society 2004

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