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Effects of dietary sucrose on hippocampal serotonin release: a microdialysis study in the freely-moving rat

Published online by Cambridge University Press:  09 March 2007

Ilse Smolders ,
jan Van Loo ,
Sophie Sarre ,
Guy Ebinger  and
Y vette Michotte
Ilse Smolders
Affiliation:
Department of Pharmaceutical Chemistry and Drug Analysis, Phamaceutical Institue, virije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
jan Van Loo
Affiliation:
ORAFTI, Aandroenstraat 1, 3300 Tienen, Belgium
Sophie Sarre
Affiliation:
Department of Pharmaceutical Chemistry and Drug Analysis, Phamaceutical Institue, virije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
Guy Ebinger
Affiliation:
Department of Neurology, University Hospital, Laarbeeklaan 101, 1090 Brussels, Belgium
Y vette Michotte*
Affiliation:
Department of Pharmaceutical Chemistry and Drug Analysis, Phamaceutical Institue, virije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
*
*Corresponding author: Professor Yvette Michotte, fax +32 2 477 41 13, email [email protected]
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Abstract

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The effects of dietary supplementation with either sucrose or starch (50 g/kg regular food for 2 weeks) on central 5-hydroxytryptamine (5HT; serotonin) release were investigated in freely-moving rats. It has been suggested that the amount of transmitter that serotoninergic neurons release might be altered by food intake. We monitored the effects of sucrose and starch on concentrations of extracellular 5HT, its metabolite 5-hydroxyindoleacetic acid (5HIAA), γ-aminobutyric acid (GABA) and dopamine in the hippocampus, using in vivo microdialysis. The major finding was that baseline levels of extracellular hippocampal 5HT in rats with ad libitum access to food supplemented with sucrose were significantly higher compared with the starch control group. We then verified that sucrose supplementation affected the potency of S(+)fenfluramine to increase hippocampal 5HT levels. In both groups of rats, acute intraperitoneal injection (1 mg/kg) of this anorectic drug induced a response curve of the extracellular hippocampal 5HT levels, with a shape that corresponded with earlier data for different brain areas often using up to 10-fold higher doses of S(+)fenfluramine. Nevertheless, we showed that throughout the experiment the absolute values of the sucrose response curve remained higher than in the starch group. On the other hand, S(+)fenfluramine exerted longer lasting effects in the starch group, as compared with the sucrose group. Significant decreases in levels of extracellular hippocampal 5HIAA levels following S(+)fenfluramine administration were simultaneously observed. A practical implication of the present findings is that dietary sucrose may bias the results of studies investigating brain serotoninergic mechanisms and the effects of (anorectic) drugs interacting with 5HT systems in the hippocampus.

Keywords

SucroseSerotoninMicrodialysis
Type
Short communication
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 151 - 155
Copyright
Copyright © The Nutrition Society 2001

References

Blundell, JE & Halford, JCG (1998) Serotonin and appetite regulation. Implications for the pharmacological treatment of obesity. CNS Drugs 9, 473–495.CrossRefGoogle Scholar
Caccia, S, Confalonieri, S, Bergami, A, Fracasso, C, Anelli, M & Garattini, S (1997) Neuropharmacological effects of low and high doses of repeated oral dexfenfluramine in rats: a comparison with fluoxetine. Pharmacology, Biochemistry and Behavior 57, 851–856.Google Scholar
Carboni, E, Cadoni, C, Tanda, GL & Di Chiara, G (1989) Calcium-dependent, tetrodotoxin-sensitive stimulation of cortical serotonin release after a tryptophan load. Journal of Neurochemistry 53, 976–978.Google Scholar
Edwards, DJ, Sorisio, DA & Knopf, S (1989) Effects of the beta 2-adrenoreceptor agonist clenbuterol on tyrosine and tryptophan in plasma and brain of the rat. Biochemical Pharmacology 38, 2957–2965.Google Scholar
Eriksson, T & Carlsson, A (1982) Isoprenaline increases brain concentrations of administered L-DOPA and L-tryptophan in the rat. Psychopharmacology (Berl) 77, 98–100.Google Scholar
Eriksson, T, Carlsson, A, Hagman, M & Jagenburg, R (1984) β-Adrenergic influence on brain concentrations of monoamine precursors. Advances in Biochemical Psychopharmacology 39, 263–270.Google Scholar
Fuller, RW (1994) Uptake inhibitors increase extracellular serotonin concentration measured by brain microdialysis. Life Sciences 55, 163–167.Google Scholar
Garattini, S, Bizzi, A, Caccia, S & Mennini, T (1992) Progress report on the anorectic effects of dexfenfluramine, fluoxetine and sertraline. International Journal of Obesity and Related Metabolic Disorders 16 Suppl. 3, S43–S50.Google Scholar
Laferrere, B & Wurtman, RJ (1989) Effect of D-fenfluramine on serotonin release in brains of anaethetized rats. Brain Research 504, 258–263.Google Scholar
Leathwood, PD (1987) Tryptophan availability and serotonin synthesis. Proceedings of the Nutrition Society 46, 143–156.Google Scholar
Paxinos, G & Watson, C (1986) The Rat Brain in Stereotaxic Coordinates, 2nd ed., San Diego, CA: Academic Press Inc.Google Scholar
Reaven, GM & Ho, H (1991) Sugar-induced hypertension in Sprague-Dawley rats. American Journal of Hypertension 4, 610–614.Google Scholar
Rouch, C, Nicolaidis, S & Orosco, M (1999) Determination, using microdialysis, of hypothalamic serotonin variations in response to different macronutrients. Physiology & Behavior 65, 653–657.Google Scholar
Sabol, KE, Richards, JB & Seiden, LS (1992) Fenfluramine-induced increases in extracellular hippocampal serotonin are progressively attenuated in vivo during a four-day fenfluramine regimen in rats. Brain Research 571, 64–72.Google Scholar
Sarre, S, Marvin, CA, Ebinger, G & Michotte, Y (1992) Microbore liquid chromatography with dual electrochemical detection for the determination of serotonin and 5-hydroxyindoleacetic acid in rat brain dialysates. Journal of Chromatography 582, 29–34.Google Scholar
Sarre, S, Smolders, I, Thorré, K, Ebinger, G & Michotte, Y (1997) Biotransformation of locally applied precursors of dopamine, serotonin and noradrenaline in striatum and hippocampus: a microdialysis study. Journal of Neural Transmission 104, 1215–1228.Google Scholar
Schwartz, DH, Hernandez, L & Hoebel, BG (1989) Fenfluramine administered systemically or locally increases extracellular serotonin in the lateral hypothalamus as measured by microdialysis. Brain Research 482, 261–270.Google Scholar
Schwartz, DH, Hernandez, L & Hoebel, BG (1990) Tryptophan increases extracellular serotonin in the lateral hypothalamus of food-deprived rats. Brain Research Bulletin 25, 803–807.Google Scholar
Sharp, T, Bramwell, SR & Grahame-Smith, DG (1992) Effect of acute administration of L-tryptophan on the release of 5HT in rat hippocampus in relation to serotoninergic neuronal activity: an in vivo microdialysis study. Life Sciences 50, 1215–1223.Google Scholar
Smolders, I, Sarre, S, Ebinger, G & Michotte, Y (1995) The analysis of excitatory, inhibitory and other amino acids in rat brain microdialysates using microbore liquid chromatography. Journal of Neuroscience Methods 57, 47–53.Google Scholar
Smolders, I, Sarre, S, Vanhaesendonck, C, Ebinger, G & Michotte, Y (1996) Extracellular striatal dopamine and glutamate after decortication and kainate receptor stimulation, as measured by microdialysis. Journal of Neurochemistry 66, 2373–2380.Google Scholar
Stancampiano, R, Melis, F, Sarais, L, Cocco, S, Cugusi, C & Fadda, F (1997) Acute administration of a tryptophan-free amino acid mixture decreases 5HT release in rat hippocampus in vivo. American Journal of Physiology 272, R991–R994.Google Scholar
Thibault, R (1994) Dietary carbohydrates: effects on self-selection, plasma glucose and insulin, and brain indoleaminergic systems in rat. Appetite 23, 275–286.Google Scholar
van Kempen, GMJ (1994) 5HT: Biochemistry, physiology and metabolism. In Serotonin (5HT) in Neurologic and Psychiatric Disorders, pp. 1–12 [Ferrari, MD, van Kempen, GMJ, Saxena, PR and Bär, PR, editors]. Leiden: Boerhaave Commissie voor Postacademisch Onderwijs in de Geneeskunde.Google Scholar
Wurtman, RJ & Wurtmann, JJ (1995) Brain serotonin, carbohydrate-craving, obesity and depression. Obesity Research 3 Suppl. 4, 477S–480S.Google Scholar
Young, JB & Landsberg, L (1977) Stimulation of the sympathetic nervous system during sucrose feeding. Nature 269, 615–617.Google Scholar
Young, SN (1991) The 1989 Borden Award Lecture. Some effects of dietary components (amino acids, carbohydrate, folic acid) on brain serotonin synthesis, mood and behavior. Canadian Journal of Physiology and Pharmacology 69, 893–903.Google Scholar