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The effects of dietary ligands on zinc uptake at the porcine intestinal brush-border membrane

Published online by Cambridge University Press:  09 March 2007

A. J. Turnbull ,
P. Blakeborough  and
R. P. H. Thompson
A. J. Turnbull
Affiliation:
Gastrointestinal Laboratory, Rayne Institute, St Thomas' Hospital, London, SEl 7EH
P. Blakeborough
Affiliation:
AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading, RG2 9AT
R. P. H. Thompson
Affiliation:
Gastrointestinal Laboratory, Rayne Institute, St Thomas' Hospital, London, SEl 7EH
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Abstract

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Intestinal brush-border-membrane vesicles were prepared from the porcine small bowel by magnesium precipitation and differential centrifugation, and were functionally intact. The influence of dietary ligands on 65Zn uptake was determined using a 65Zn concentration of 5 μm, an incubation time of 1 min and a reaction temperature of 27°, with a rapid filtration technique. At this low Zn concentration the addition of an excess of folate, histidine or glucose had no effect on Zn uptake. Addition of picolinate, citrate and phytate to the incubation medium significantly reduced Zn uptake at all concentrations of ligand examined. Any inhibitory effects of folic acid in vivo may thuss be due to a mucosal rather than lumen interaction. Those ligands inhibiting absorption may have done so through the formation of Zn-ligand complexes, which are either insoluble, or which reduce the binding of Zn to its mucosal receptor. This in vitro model of Zn absorption is useful for comparing the effects of potential Zn-binding ligands in the diet.

Keywords

Zinc absorptionLigandsPig
Type
Ligands and Zinc Absorption
Information
British Journal of Nutrition , Volume 64 , Issue 3 , November 1990 , pp. 733 - 741
Copyright
Copyright © The Nutrition Society 1990

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