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Effects of dietary fats (fish, olive and high-oleic-acid sunflower oils) on lipid composition and antioxidant enzymes in rat liver

Published online by Cambridge University Press:  09 March 2007

Valentina Ruiz-Gutiérrez ,
Alonso Pérez-Espinosa ,
Carmen María Vázquez  and
Consuelo Santa-María
Valentina Ruiz-Gutiérrez
Affiliation:
Instituto de la Grasa y sus Derivados, CSIC, apdo 1078, Sevilla, Spain
Alonso Pérez-Espinosa
Affiliation:
Instituto de la Grasa y sus Derivados, CSIC, apdo 1078, Sevilla, Spain
Carmen María Vázquez
Affiliation:
Departamento Fisiología y Biología Animal, Facultad de Farmacia, c/ Professor García González s/n, 41012, Sevilla, Spain
Consuelo Santa-María*
Affiliation:
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, c/ Professor García González s/n, 41012, Sevilla, Spain
*
*Corresponding author: Dr Consuelo Santa-María, fax +34 95 423 3765, email [email protected]
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Abstract

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The effects of two oleic-acid-rich diets (containing olive oil, OO, and high-oleic-acid sunflower oil, HOSO) on plasma and liver lipid composition detoxification enzyme activities, were compared with those of a fish-oil (FO) diet and a control diet. Compared with the control diet, plasma and hepatic total triacylglycerol concentrations were increased in the animals fed on the HOSO and OO diets and decreased in those fed on the FO diet. The animals fed on FO showed the highest level of cholesterol in the liver and had lower plasma cholesterol concentrations when compared with those fed on the two oleic-acid-rich diets. In comparison with the animals fed on the diets enriched in oleic acid, the FO group showed higher hepatic levels of polyunsaturated fatty acids of the n-3 series and lower levels of fatty acids of the n-6 series. Livers of FO-fed rats, compared with those of OO- and HOSO-fed rats showed: (1) significantly higher activities of catalase (EC 1.11.1.6) glutathione peroxidase (EC 1.11.1.9) and Cu/Zn superoxide dismutase (EC 1.15.1.1); (2) no differences in the NADPH-cytochrome c reductase (EC 1.6.99.3) activity. The HOSO diet had a similar effect on liver antioxidant enzyme activities as the OO diet. In conclusion, it appears that changes in the liver fatty acid composition due mainly to n-3 lipids may enhance the efficiency of the antioxidant defence system. The two monounsaturated fatty acids oils studied (OO and HOSO), with the same high content of oleic acid but different contents of natural antioxidants, had similar effects on the antioxidant enzyme activities measured.

Keywords

Fatty acidsAntioxidant enzymesOleic acid
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 3 , September 1999 , pp. 233 - 241
Copyright
Copyright © The Nutrition Society 1999

References

Allain, CC, Poon, LS, Chang, CSG, Richmond, W & Fu, PC (1974) Enzymatic determination of total plasma cholesterol. Clinical Chemistry 20, 470–475.CrossRefGoogle Scholar
Arce, L, Rios, A & Valcarcel, M (1998) Determination of anti-carcinogenic polyphenols present in green tea using capillary electrophoresis coupled to a flow injection system. Journal of Chromatography A 827, 113–120.CrossRefGoogle ScholarPubMed
Archer, S, Nelson, D, Gebhard, R, Levine, AS, Prigge, W & Weir, EK (1987) Effects of dietary fish oil on lung phospholipid fatty acid composition and intrinsic pulmonary vascular reactivity. Cardiovascular Research 21, 928–932.CrossRefGoogle ScholarPubMed
Bang, HO, Dyerberg, J & Nielsen, AB (1986) Plasma lipid and lipoprotein pattern in Groenlandic West-coast Eskimos. Nutrition Reviews 44, 143–146.CrossRefGoogle Scholar
Beers, RF & Sizer, IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry 195, 133–140.CrossRefGoogle ScholarPubMed
Berge, RK & Thomassen, MS (1985) Effects of high-fat diets on the activity of palmitoyl-CoA hydrolase in rat liver. Lipids 20, 49–52.CrossRefGoogle ScholarPubMed
Bucolo, G & David, H (1973) Quantitative determination of plasma triglycerides by use of enzymes. Clinical Chemistry 19, 476–482.CrossRefGoogle Scholar
Ceriello, A, dello Russo, P, Amstad, P & Cerutti, P (1996) High glucose induces antioxidant enzymes in human endothelial cells in culture. Evidence linking hyperglycemia and oxidative stress. Diabetics 45, 471–477.Google ScholarPubMed
Cramer, CT, Cooke, S, Ginsberg, LC, Kletzien, RF, Stapleton, SR & Ulrich, RG (1995) Upregulation of glucose-6-phosphate dehydrogenase in response to hepatocellular oxidative stress: studies with diquat. Journal of Biochemical Toxicology 10, 293–298.CrossRefGoogle ScholarPubMed
De Haan, JB, Newman, JD & Kola, I (1992) Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with ageing in murine brains. Brain Research-Molecular Brain Research 13, 179–187.CrossRefGoogle ScholarPubMed
De Schrijver, R & Vermeulen, D (1991) Separation and quantification of phospholipids in animal tissues by Iatroscan TLC/FID. Lipids 26, 74–75.CrossRefGoogle Scholar
Fang, JL, Vaca, CE, Valsta, LM & Mutanen, M (1996) Determination of DNA adducts of malonaldehyde in humans: effects of dietary fatty acid composition. Carcinogenesis 17, 1035–1040.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Froyland, L, Madsen, L, Vaagenes, H, Totland, GK, Auwerx, J, Kryvi, H, Staels, B & Berge, RK (1997) Mitochondrion is the principal target for nutritional and pharmacological control of triglyceride metabolism. Journal of Lipid Research 38, 1851–1858.CrossRefGoogle ScholarPubMed
Garcia Regueiro, JA, Gilbert, J & Diaz, I (1994) Determination of neutral lipids from subcutaneous fat of cured ham by capillary gas chromatography and liquid chromatography. Journal of Chromatography A 667, 225–233.CrossRefGoogle ScholarPubMed
Garg, ML, Sebokova, E, Wierzbicki, A, Thomson, AB, Thomson, R & Clandinin, MT (1988) Differential effects of dietary linoleic and α-linolenic acid on lipid metabolism in rat tissues. Lipids 23, 847–852.CrossRefGoogle ScholarPubMed
Harman, D (1992) Free radical theory of ageing. Mutation Research 275, 257–266.CrossRefGoogle Scholar
Herold, PM & Kinsella, JE (1986) Fish oil consumption and decreased risk of cardiovascular disease: a comparison of findings from animal and human feeding trials. American Journal of Clinical Nutrition 43, 566–598.CrossRefGoogle ScholarPubMed
Huang, CY, Chen, LH, Osio, Y & Cohen, DA (1994) Effects of diet composition on liver antioxidant defence and detoxification enzymes in mice with murine AIDS. Nutrition Research 14, 1841–1851.CrossRefGoogle Scholar
Huff, MW, Telford, DE, Edmonds, BW, McDonalds, CG & Evans, AJ (1993) Lipoprotein lipases, lipoprotein density gradient profile and LDL receptor activity in miniature pigs fed fish oil and corn oil. Biochimica et Biophysica Acta 1210, 113–122.CrossRefGoogle ScholarPubMed
Jeffery, NM, Yaqoob, P, Newsholme, EA & Calder, PC (1996) The effects of olive oil upon rat serum lipid levels and lymphocyte functions appear to be due to oleic acid. Annals of Nutrition and Metabolism 40, 71–80.CrossRefGoogle ScholarPubMed
Jeng, KC & Fernandes, G (1989) Effect of fish oil diet on immune response and proteinuria in mice. International Journal of Immunopathology and Immunopharmacology 126, 1999–2006.Google Scholar
Kok, TM, Vaarwerk, F, Zwingman, I, Maanen, JM & Kleinjans, JC (1994) Peroxidation of linoleic, arachidonic and oleic acid in relation to the induction of oxidative DNA damage and cytogenetic effects. Carcinogenesis 15, 1399–1404.CrossRefGoogle Scholar
Kramer, JK, Blais, L, Fouchard, RC, Melnyk, RA & Kallery, KM (1997) A rapid method for the determination of vitamin E forms in tissues and diet by high-performance liquid chromatography using a normal-phase diol column. Lipids 32, 323–330.CrossRefGoogle ScholarPubMed
Kromhout, D, Bosschieter, EB & Coulander, CL (1985) The inverse relation between fish consumption and 20-year mortality from coronary heart disease. New England Journal of Medicine 312, 1205–1209.CrossRefGoogle ScholarPubMed
L'Abbe, MR, Trick, KD & Beare-Rogers, JL (1991) Dietary (n-3) fatty acids affect rat heart, liver and aorta protective enzyme activities and lipid peroxidation. Journal of Nutrition 121, 1331–1340.CrossRefGoogle ScholarPubMed
Larrea, E, Beloqui, O, Muñoz-Navas, MA, Civeira, MP & Prieto, J (1998) Superoxide dismutase in patients with chronic hepatitis C virus infection. Free Radical Biology and Medicine 24, 1235–1241.CrossRefGoogle ScholarPubMed
Lawrence, A & Burk, RF (1976) Glutathione peroxidase activity in selenium deficient rat liver. Biochemical and Biophysical Research Communications 71, 952–958.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RL (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
McCord, JM & Fridovich, I (1969) Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244, 6049–6055.CrossRefGoogle Scholar
Masi, I, Giani, E, Galli, C, Tremoli, E & Sirtori, CR (1986) Diets rich in saturated, monounsaturated and polyunsaturated fatty acids differently affect plasma lipid, platelet and arterial wall eicosanoids in rabbits. Annals of Nutrition and Metabolism 30, 66–72.CrossRefGoogle Scholar
Mattson, FH & Grundy, SM (1985) Comparison of effects of dietary saturated, monounsaturated and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. Journal of Lipid Research 26, 194–202.CrossRefGoogle ScholarPubMed
Molina, MT, Vázquez, CM & Ruiz-Gutiérrez, V (1989) Changes in both acyl-CoA:cholesterol acyltransferase activity and microsomal lipid composition in rat liver induced by distal small-bowel resection. Biochemical Journal 260, 115–119.CrossRefGoogle ScholarPubMed
Nalbone, G, Leonardi, J, Termine, E, Portugal, H, Lechene, P, Pauli, A & Lafont, H (1980) Effects of fish oil, corn oil and lard diets on lipid peroxidation status and glutathione peroxidase activities in rat heart. Lipids 24, 179–186.CrossRefGoogle Scholar
Nelson, GJ (1972) Quantitative analysis of bloods lipids. In Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism, pp. 171–198 [Nelson, GJ, editor]. New York, NY: Wiley-Interscience.Google Scholar
Papadopoulos, G & Boskou, D (1991) Antioxidant effect of natural phenols in olive. Journal of the American Oil Chemists' Society 68, 669–671.CrossRefGoogle Scholar
Pérez-Jiménez, F, Espino, A, López-Segura, F, Blanco, J, Ruiz-Gutiérrez, V, Prada, JLLópez Miranda, JJiménez-Perezpérez, J & Ordovas, JM (1995) Lipoprotein concentrations in normolipidemic males consuming oleic acid-rich diets from two different sources: olive oil and oleic-acid-rich sunflower oil. American Journal of Clinical Nutrition 62, 769–775.CrossRefGoogle ScholarPubMed
Perona, JS & Ruiz-Gutierrez, V (1998) Two highly monounsaturated oils, olive oil and high-oleic sunflower oil, induce different triacylglycerol molecular species distribution in rat liver. Nutrition Research 18, 1723–1732.CrossRefGoogle Scholar
Phillipson, BE, Rothrock, DW, Connor, WE, Harris, WS & Illingworth, DR (1985) Reduction of plasma lipids, lipoproteins and apoproteins by dietary fish oils in patients with hypertriglyceridemia. New England Journal of Medicine 312, 1210–1216.CrossRefGoogle ScholarPubMed
Rao, G, Xia, E & Richardson, A (1990) Effect of age on the expression of antioxidant enzymes in male Fischer F 344 rats. Mechanisms in Ageing Development 53, 49–60.CrossRefGoogle Scholar
Roche, E & Romero-Alvira, D (1995) Role of oxidative stress in gene expression: myocardial and cerebral ischemia, cancer and other diseases. Medicina Clinica 104, 468–476.Google ScholarPubMed
Semsei, I, Rao, G & Richardson, A (1989) Changes in the expression of superoxide dismutase and catalase as a function of age and dietary restriction. Biochemical and Biophysical Research Communications 164, 620–625.CrossRefGoogle ScholarPubMed
Smith, MT & Willis, ED (1981) The effects of dietary lipid and phenobarbitone on the production and utilization of NADPH in the liver. Biochemical Journal 200, 691–699.CrossRefGoogle ScholarPubMed
Spector, AA & Yorek, MA (1985) Membrane lipid composition and cellular function. Journal of Lipid Research 26, 1015–1035.CrossRefGoogle ScholarPubMed
Sulpice, JC & Ferezou, J (1984) Squalene isolation by HPLC and quantitative comparison by HPLC and GLC. Lipids 19, 631–635.CrossRefGoogle ScholarPubMed
Ulloa, N & Nervi, F (1985) Mechanism and kinetic characteristics of the uncoupling by plant steroids of biliary cholesterol from bile salt output. Biochimica et Biophysica Acta 837, 181–189.CrossRefGoogle ScholarPubMed
Van Noorden, CJF (1995) Effects of n-3 and n-6 polyunsaturated fatty acid-enriched diets on lipid metabolism in periportal and pericentral compartments of female rat liver lobules and the consequences for cell proliferation after partial hepatectomy. Journal of Lipid Research 36, 1708–1720.CrossRefGoogle ScholarPubMed
Venkatraman, JL, Angkeow, P, Satsangi, N & Fernandes, G (1998) Effects of dietary n-6 and n-3 lipids on antioxidant defence system in livers of exercised rats. Journal of the American College of Nutrition 17, 586–594.CrossRefGoogle ScholarPubMed
Venkatraman, JT, Chandrasekar, B, Kim, JD & Fernandes, G (1994) Effects of n-3 and n-6 fatty acids on the activities and expression of hepatic antioxidant enzymes in autoimmune-prone NZB × NZW F1 mice. Lipids 29, 561–568.CrossRefGoogle Scholar
Vermilion, JL & Coon, MJ (1978) Purified liver microsomal NADPH-cytochrome P450 reductase. Spectral characterization of oxidation–reduction states. Journal of Biological Chemistry 253, 2694–2704.CrossRefGoogle ScholarPubMed
Wong, SH, Nestel, PJ, Trimble, RP, Store, GB, Illman, RJ & Topping, DL (1984) The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver. Biochimica et Biophysica Acta 792, 103–109.CrossRefGoogle ScholarPubMed
Yamazaki, RK, Shen, T & Schade, GB (1987) A diet rich in (n-3) fatty acids increases peroxisomal β-oxidation activity and lowers plasma triacylglycerols without inhibiting glutathione-dependent detoxification activities in the rat liver. Biochimica et Biophysica Acta 920, 62–67.CrossRefGoogle Scholar
Yaqoob, P, Sherrington, EJ, Jeffery, NM, Sanderson, P, Harvey, DJ, Newsholme, EA & Calder, PC (1995) Comparison of the effects of a range of dietary lipids upon serum and tissue lipid composition in the rat. International Journal of Biochemistry 27, 297–310.Google ScholarPubMed